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Genome-Wide Identification and Expression Analyses of Aquaporin Gene Family during Development and Abiotic Stress in Banana.

Hu W, Hou X, Huang C, Yan Y, Tie W, Ding Z, Wei Y, Liu J, Miao H, Lu Z, Li M, Xu B, Jin Z - Int J Mol Sci (2015)

Bottom Line: Expression analysis of MaAQP genes during fruit development and postharvest ripening showed that some MaAQP genes exhibited high expression levels during these stages, indicating the involvement of MaAQP genes in banana fruit development and ripening.Additionally, some MaAQP genes showed strong induction after stress treatment and therefore, may represent potential candidates for improving banana resistance to abiotic stress.Taken together, this study identified some excellent tissue-specific, fruit development- and ripening-dependent, and abiotic stress-responsive candidate MaAQP genes, which could lay a solid foundation for genetic improvement of banana cultivars.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, China. huwei2010916@126.com.

ABSTRACT
Aquaporins (AQPs) function to selectively control the flow of water and other small molecules through biological membranes, playing crucial roles in various biological processes. However, little information is available on the AQP gene family in bananas. In this study, we identified 47 banana AQP genes based on the banana genome sequence. Evolutionary analysis of AQPs from banana, Arabidopsis, poplar, and rice indicated that banana AQPs (MaAQPs) were clustered into four subfamilies. Conserved motif analysis showed that all banana AQPs contained the typical AQP-like or major intrinsic protein (MIP) domain. Gene structure analysis suggested the majority of MaAQPs had two to four introns with a highly specific number and length for each subfamily. Expression analysis of MaAQP genes during fruit development and postharvest ripening showed that some MaAQP genes exhibited high expression levels during these stages, indicating the involvement of MaAQP genes in banana fruit development and ripening. Additionally, some MaAQP genes showed strong induction after stress treatment and therefore, may represent potential candidates for improving banana resistance to abiotic stress. Taken together, this study identified some excellent tissue-specific, fruit development- and ripening-dependent, and abiotic stress-responsive candidate MaAQP genes, which could lay a solid foundation for genetic improvement of banana cultivars.

No MeSH data available.


Expression profiles of MaAQP genes in roots, leaves, and fruits of two cultivated banana varieties. The heat-map was established based on MeV software. The scale represents signal intensity of FPKM values.
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ijms-16-19728-f005: Expression profiles of MaAQP genes in roots, leaves, and fruits of two cultivated banana varieties. The heat-map was established based on MeV software. The scale represents signal intensity of FPKM values.

Mentions: To provide some clues on the roles of MaAQP genes in banana growth and development, expression profiles of AQP genes in different organs, including roots, leaves, and fruits, were tested in two cultivated banana varieties, BaXi Jiao (Musa acuminate L. AAA group cv. Cavendish; hereafter referred to as BX) and Fen Jiao (Musa ABB Pisang Awak; hereafter referred to as FJ). The transcripts of 40 MaAQP genes (except for MaPIP2-9, MaPIP1-9, MaPIP1-3, MaPIP1-2, MaTIP3-1, MaNIP1-2 and MaNIP2-5) were captured based on transcriptomic data from each organ, and then a heat-map with phylogenetic analysis was produced to show the expression patterns of MaAQP genes in different organs (Figure 5, Table S8).


Genome-Wide Identification and Expression Analyses of Aquaporin Gene Family during Development and Abiotic Stress in Banana.

Hu W, Hou X, Huang C, Yan Y, Tie W, Ding Z, Wei Y, Liu J, Miao H, Lu Z, Li M, Xu B, Jin Z - Int J Mol Sci (2015)

Expression profiles of MaAQP genes in roots, leaves, and fruits of two cultivated banana varieties. The heat-map was established based on MeV software. The scale represents signal intensity of FPKM values.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581322&req=5

ijms-16-19728-f005: Expression profiles of MaAQP genes in roots, leaves, and fruits of two cultivated banana varieties. The heat-map was established based on MeV software. The scale represents signal intensity of FPKM values.
Mentions: To provide some clues on the roles of MaAQP genes in banana growth and development, expression profiles of AQP genes in different organs, including roots, leaves, and fruits, were tested in two cultivated banana varieties, BaXi Jiao (Musa acuminate L. AAA group cv. Cavendish; hereafter referred to as BX) and Fen Jiao (Musa ABB Pisang Awak; hereafter referred to as FJ). The transcripts of 40 MaAQP genes (except for MaPIP2-9, MaPIP1-9, MaPIP1-3, MaPIP1-2, MaTIP3-1, MaNIP1-2 and MaNIP2-5) were captured based on transcriptomic data from each organ, and then a heat-map with phylogenetic analysis was produced to show the expression patterns of MaAQP genes in different organs (Figure 5, Table S8).

Bottom Line: Expression analysis of MaAQP genes during fruit development and postharvest ripening showed that some MaAQP genes exhibited high expression levels during these stages, indicating the involvement of MaAQP genes in banana fruit development and ripening.Additionally, some MaAQP genes showed strong induction after stress treatment and therefore, may represent potential candidates for improving banana resistance to abiotic stress.Taken together, this study identified some excellent tissue-specific, fruit development- and ripening-dependent, and abiotic stress-responsive candidate MaAQP genes, which could lay a solid foundation for genetic improvement of banana cultivars.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, China. huwei2010916@126.com.

ABSTRACT
Aquaporins (AQPs) function to selectively control the flow of water and other small molecules through biological membranes, playing crucial roles in various biological processes. However, little information is available on the AQP gene family in bananas. In this study, we identified 47 banana AQP genes based on the banana genome sequence. Evolutionary analysis of AQPs from banana, Arabidopsis, poplar, and rice indicated that banana AQPs (MaAQPs) were clustered into four subfamilies. Conserved motif analysis showed that all banana AQPs contained the typical AQP-like or major intrinsic protein (MIP) domain. Gene structure analysis suggested the majority of MaAQPs had two to four introns with a highly specific number and length for each subfamily. Expression analysis of MaAQP genes during fruit development and postharvest ripening showed that some MaAQP genes exhibited high expression levels during these stages, indicating the involvement of MaAQP genes in banana fruit development and ripening. Additionally, some MaAQP genes showed strong induction after stress treatment and therefore, may represent potential candidates for improving banana resistance to abiotic stress. Taken together, this study identified some excellent tissue-specific, fruit development- and ripening-dependent, and abiotic stress-responsive candidate MaAQP genes, which could lay a solid foundation for genetic improvement of banana cultivars.

No MeSH data available.