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Genome-Wide Identification and Expression Analyses of Aquaporin Gene Family during Development and Abiotic Stress in Banana.

Hu W, Hou X, Huang C, Yan Y, Tie W, Ding Z, Wei Y, Liu J, Miao H, Lu Z, Li M, Xu B, Jin Z - Int J Mol Sci (2015)

Bottom Line: Expression analysis of MaAQP genes during fruit development and postharvest ripening showed that some MaAQP genes exhibited high expression levels during these stages, indicating the involvement of MaAQP genes in banana fruit development and ripening.Additionally, some MaAQP genes showed strong induction after stress treatment and therefore, may represent potential candidates for improving banana resistance to abiotic stress.Taken together, this study identified some excellent tissue-specific, fruit development- and ripening-dependent, and abiotic stress-responsive candidate MaAQP genes, which could lay a solid foundation for genetic improvement of banana cultivars.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, China. huwei2010916@126.com.

ABSTRACT
Aquaporins (AQPs) function to selectively control the flow of water and other small molecules through biological membranes, playing crucial roles in various biological processes. However, little information is available on the AQP gene family in bananas. In this study, we identified 47 banana AQP genes based on the banana genome sequence. Evolutionary analysis of AQPs from banana, Arabidopsis, poplar, and rice indicated that banana AQPs (MaAQPs) were clustered into four subfamilies. Conserved motif analysis showed that all banana AQPs contained the typical AQP-like or major intrinsic protein (MIP) domain. Gene structure analysis suggested the majority of MaAQPs had two to four introns with a highly specific number and length for each subfamily. Expression analysis of MaAQP genes during fruit development and postharvest ripening showed that some MaAQP genes exhibited high expression levels during these stages, indicating the involvement of MaAQP genes in banana fruit development and ripening. Additionally, some MaAQP genes showed strong induction after stress treatment and therefore, may represent potential candidates for improving banana resistance to abiotic stress. Taken together, this study identified some excellent tissue-specific, fruit development- and ripening-dependent, and abiotic stress-responsive candidate MaAQP genes, which could lay a solid foundation for genetic improvement of banana cultivars.

No MeSH data available.


Motif analyses of banana AQPs. All motifs were identified by the MEME database with complete amino acid sequences of MaAQPs. Lengths of motifs for each MaAQP protein are displayed proportionally.
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ijms-16-19728-f004: Motif analyses of banana AQPs. All motifs were identified by the MEME database with complete amino acid sequences of MaAQPs. Lengths of motifs for each MaAQP protein are displayed proportionally.

Mentions: To detect the structural diversity and predict the function of MaAQP proteins, a total of 15 conserved motifs in banana AQPs were found by MEME software and further annotated by InterPro Scan 5 (Figure 4, Table S7). The results showed that nine (1–9) out of 15 motifs were annotated as AQP-like or MIP domains, which are basic characteristics of the AQP family. All MaAQP proteins identified contained these conserved domains. All AQPs in the PIP subfamily contained motifs 1–5. Moreover, closely related MaPIP2-6 and MaPIP2-7 also had motifs 7 and 13, in addition to motifs 1–5. In the TIP subfamily, all members contained motifs 1, 2, 6–8, 10, and 14. In the SIP subfamily, all three members contained motifs 2 and 8. In the NIP subfamily, all members had motifs 1, 2, and 8–10. Thus, each subfamily of banana AQP shared the same conserved motifs, which supports the phylogenetic classification of the banana AQP family.


Genome-Wide Identification and Expression Analyses of Aquaporin Gene Family during Development and Abiotic Stress in Banana.

Hu W, Hou X, Huang C, Yan Y, Tie W, Ding Z, Wei Y, Liu J, Miao H, Lu Z, Li M, Xu B, Jin Z - Int J Mol Sci (2015)

Motif analyses of banana AQPs. All motifs were identified by the MEME database with complete amino acid sequences of MaAQPs. Lengths of motifs for each MaAQP protein are displayed proportionally.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581322&req=5

ijms-16-19728-f004: Motif analyses of banana AQPs. All motifs were identified by the MEME database with complete amino acid sequences of MaAQPs. Lengths of motifs for each MaAQP protein are displayed proportionally.
Mentions: To detect the structural diversity and predict the function of MaAQP proteins, a total of 15 conserved motifs in banana AQPs were found by MEME software and further annotated by InterPro Scan 5 (Figure 4, Table S7). The results showed that nine (1–9) out of 15 motifs were annotated as AQP-like or MIP domains, which are basic characteristics of the AQP family. All MaAQP proteins identified contained these conserved domains. All AQPs in the PIP subfamily contained motifs 1–5. Moreover, closely related MaPIP2-6 and MaPIP2-7 also had motifs 7 and 13, in addition to motifs 1–5. In the TIP subfamily, all members contained motifs 1, 2, 6–8, 10, and 14. In the SIP subfamily, all three members contained motifs 2 and 8. In the NIP subfamily, all members had motifs 1, 2, and 8–10. Thus, each subfamily of banana AQP shared the same conserved motifs, which supports the phylogenetic classification of the banana AQP family.

Bottom Line: Expression analysis of MaAQP genes during fruit development and postharvest ripening showed that some MaAQP genes exhibited high expression levels during these stages, indicating the involvement of MaAQP genes in banana fruit development and ripening.Additionally, some MaAQP genes showed strong induction after stress treatment and therefore, may represent potential candidates for improving banana resistance to abiotic stress.Taken together, this study identified some excellent tissue-specific, fruit development- and ripening-dependent, and abiotic stress-responsive candidate MaAQP genes, which could lay a solid foundation for genetic improvement of banana cultivars.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, China. huwei2010916@126.com.

ABSTRACT
Aquaporins (AQPs) function to selectively control the flow of water and other small molecules through biological membranes, playing crucial roles in various biological processes. However, little information is available on the AQP gene family in bananas. In this study, we identified 47 banana AQP genes based on the banana genome sequence. Evolutionary analysis of AQPs from banana, Arabidopsis, poplar, and rice indicated that banana AQPs (MaAQPs) were clustered into four subfamilies. Conserved motif analysis showed that all banana AQPs contained the typical AQP-like or major intrinsic protein (MIP) domain. Gene structure analysis suggested the majority of MaAQPs had two to four introns with a highly specific number and length for each subfamily. Expression analysis of MaAQP genes during fruit development and postharvest ripening showed that some MaAQP genes exhibited high expression levels during these stages, indicating the involvement of MaAQP genes in banana fruit development and ripening. Additionally, some MaAQP genes showed strong induction after stress treatment and therefore, may represent potential candidates for improving banana resistance to abiotic stress. Taken together, this study identified some excellent tissue-specific, fruit development- and ripening-dependent, and abiotic stress-responsive candidate MaAQP genes, which could lay a solid foundation for genetic improvement of banana cultivars.

No MeSH data available.