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Genome-Wide Identification and Expression Analyses of Aquaporin Gene Family during Development and Abiotic Stress in Banana.

Hu W, Hou X, Huang C, Yan Y, Tie W, Ding Z, Wei Y, Liu J, Miao H, Lu Z, Li M, Xu B, Jin Z - Int J Mol Sci (2015)

Bottom Line: Expression analysis of MaAQP genes during fruit development and postharvest ripening showed that some MaAQP genes exhibited high expression levels during these stages, indicating the involvement of MaAQP genes in banana fruit development and ripening.Additionally, some MaAQP genes showed strong induction after stress treatment and therefore, may represent potential candidates for improving banana resistance to abiotic stress.Taken together, this study identified some excellent tissue-specific, fruit development- and ripening-dependent, and abiotic stress-responsive candidate MaAQP genes, which could lay a solid foundation for genetic improvement of banana cultivars.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, China. huwei2010916@126.com.

ABSTRACT
Aquaporins (AQPs) function to selectively control the flow of water and other small molecules through biological membranes, playing crucial roles in various biological processes. However, little information is available on the AQP gene family in bananas. In this study, we identified 47 banana AQP genes based on the banana genome sequence. Evolutionary analysis of AQPs from banana, Arabidopsis, poplar, and rice indicated that banana AQPs (MaAQPs) were clustered into four subfamilies. Conserved motif analysis showed that all banana AQPs contained the typical AQP-like or major intrinsic protein (MIP) domain. Gene structure analysis suggested the majority of MaAQPs had two to four introns with a highly specific number and length for each subfamily. Expression analysis of MaAQP genes during fruit development and postharvest ripening showed that some MaAQP genes exhibited high expression levels during these stages, indicating the involvement of MaAQP genes in banana fruit development and ripening. Additionally, some MaAQP genes showed strong induction after stress treatment and therefore, may represent potential candidates for improving banana resistance to abiotic stress. Taken together, this study identified some excellent tissue-specific, fruit development- and ripening-dependent, and abiotic stress-responsive candidate MaAQP genes, which could lay a solid foundation for genetic improvement of banana cultivars.

No MeSH data available.


Exon-intron structure analyses of banana AQPs. Exon-intron structure analyses were conducted using the GSDS database. Lengths of exons and introns of each MaAQP gene are displayed proportionally.
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ijms-16-19728-f003: Exon-intron structure analyses of banana AQPs. Exon-intron structure analyses were conducted using the GSDS database. Lengths of exons and introns of each MaAQP gene are displayed proportionally.

Mentions: Exon-intron structural diversity often plays a key role in the evolution of gene families and can provide additional evidence to support phylogenetic groupings [29,30]. The exon-intron structure of MaAQP genes was detected according to their evolutionary classification. As shown in Figure 3, divergence of the AQP family is consistent with classification results presented in Figure 2. All MaPIP genes had three introns. Thirteen of the 17 MaTIP genes had two introns, while the remaining four MaTIPs contained only one intron. The number of introns in MaNIPs ranged from three to four. MaNIP1-1, MaNIP1-2, and MaNIP2-1, -2, -3, -4, and -5 had four introns; and MaNIP3-2 and MaNIP4-1 contained three. All MaSIP genes contained two long introns. Within this frame, paralogous gene pairs generally shared highly similar exon-intron structures, such as MaPIP2-6 and MaPIP2-7, MaPIP1-3 and MaPIP1-4, and MaTIP1-1 and MaTIP1-3. On the whole, the number and length of exons and introns was highly specific for each subfamily (Figure 3). The divergent gene structures among the different phylogenetic subgroups suggest that genes evolved into diverse exon-intron structures to accomplish different functions in the banana genome.


Genome-Wide Identification and Expression Analyses of Aquaporin Gene Family during Development and Abiotic Stress in Banana.

Hu W, Hou X, Huang C, Yan Y, Tie W, Ding Z, Wei Y, Liu J, Miao H, Lu Z, Li M, Xu B, Jin Z - Int J Mol Sci (2015)

Exon-intron structure analyses of banana AQPs. Exon-intron structure analyses were conducted using the GSDS database. Lengths of exons and introns of each MaAQP gene are displayed proportionally.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581322&req=5

ijms-16-19728-f003: Exon-intron structure analyses of banana AQPs. Exon-intron structure analyses were conducted using the GSDS database. Lengths of exons and introns of each MaAQP gene are displayed proportionally.
Mentions: Exon-intron structural diversity often plays a key role in the evolution of gene families and can provide additional evidence to support phylogenetic groupings [29,30]. The exon-intron structure of MaAQP genes was detected according to their evolutionary classification. As shown in Figure 3, divergence of the AQP family is consistent with classification results presented in Figure 2. All MaPIP genes had three introns. Thirteen of the 17 MaTIP genes had two introns, while the remaining four MaTIPs contained only one intron. The number of introns in MaNIPs ranged from three to four. MaNIP1-1, MaNIP1-2, and MaNIP2-1, -2, -3, -4, and -5 had four introns; and MaNIP3-2 and MaNIP4-1 contained three. All MaSIP genes contained two long introns. Within this frame, paralogous gene pairs generally shared highly similar exon-intron structures, such as MaPIP2-6 and MaPIP2-7, MaPIP1-3 and MaPIP1-4, and MaTIP1-1 and MaTIP1-3. On the whole, the number and length of exons and introns was highly specific for each subfamily (Figure 3). The divergent gene structures among the different phylogenetic subgroups suggest that genes evolved into diverse exon-intron structures to accomplish different functions in the banana genome.

Bottom Line: Expression analysis of MaAQP genes during fruit development and postharvest ripening showed that some MaAQP genes exhibited high expression levels during these stages, indicating the involvement of MaAQP genes in banana fruit development and ripening.Additionally, some MaAQP genes showed strong induction after stress treatment and therefore, may represent potential candidates for improving banana resistance to abiotic stress.Taken together, this study identified some excellent tissue-specific, fruit development- and ripening-dependent, and abiotic stress-responsive candidate MaAQP genes, which could lay a solid foundation for genetic improvement of banana cultivars.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, China. huwei2010916@126.com.

ABSTRACT
Aquaporins (AQPs) function to selectively control the flow of water and other small molecules through biological membranes, playing crucial roles in various biological processes. However, little information is available on the AQP gene family in bananas. In this study, we identified 47 banana AQP genes based on the banana genome sequence. Evolutionary analysis of AQPs from banana, Arabidopsis, poplar, and rice indicated that banana AQPs (MaAQPs) were clustered into four subfamilies. Conserved motif analysis showed that all banana AQPs contained the typical AQP-like or major intrinsic protein (MIP) domain. Gene structure analysis suggested the majority of MaAQPs had two to four introns with a highly specific number and length for each subfamily. Expression analysis of MaAQP genes during fruit development and postharvest ripening showed that some MaAQP genes exhibited high expression levels during these stages, indicating the involvement of MaAQP genes in banana fruit development and ripening. Additionally, some MaAQP genes showed strong induction after stress treatment and therefore, may represent potential candidates for improving banana resistance to abiotic stress. Taken together, this study identified some excellent tissue-specific, fruit development- and ripening-dependent, and abiotic stress-responsive candidate MaAQP genes, which could lay a solid foundation for genetic improvement of banana cultivars.

No MeSH data available.