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Genome-Wide Identification and Expression Analyses of Aquaporin Gene Family during Development and Abiotic Stress in Banana.

Hu W, Hou X, Huang C, Yan Y, Tie W, Ding Z, Wei Y, Liu J, Miao H, Lu Z, Li M, Xu B, Jin Z - Int J Mol Sci (2015)

Bottom Line: Expression analysis of MaAQP genes during fruit development and postharvest ripening showed that some MaAQP genes exhibited high expression levels during these stages, indicating the involvement of MaAQP genes in banana fruit development and ripening.Additionally, some MaAQP genes showed strong induction after stress treatment and therefore, may represent potential candidates for improving banana resistance to abiotic stress.Taken together, this study identified some excellent tissue-specific, fruit development- and ripening-dependent, and abiotic stress-responsive candidate MaAQP genes, which could lay a solid foundation for genetic improvement of banana cultivars.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, China. huwei2010916@126.com.

ABSTRACT
Aquaporins (AQPs) function to selectively control the flow of water and other small molecules through biological membranes, playing crucial roles in various biological processes. However, little information is available on the AQP gene family in bananas. In this study, we identified 47 banana AQP genes based on the banana genome sequence. Evolutionary analysis of AQPs from banana, Arabidopsis, poplar, and rice indicated that banana AQPs (MaAQPs) were clustered into four subfamilies. Conserved motif analysis showed that all banana AQPs contained the typical AQP-like or major intrinsic protein (MIP) domain. Gene structure analysis suggested the majority of MaAQPs had two to four introns with a highly specific number and length for each subfamily. Expression analysis of MaAQP genes during fruit development and postharvest ripening showed that some MaAQP genes exhibited high expression levels during these stages, indicating the involvement of MaAQP genes in banana fruit development and ripening. Additionally, some MaAQP genes showed strong induction after stress treatment and therefore, may represent potential candidates for improving banana resistance to abiotic stress. Taken together, this study identified some excellent tissue-specific, fruit development- and ripening-dependent, and abiotic stress-responsive candidate MaAQP genes, which could lay a solid foundation for genetic improvement of banana cultivars.

No MeSH data available.


Phylogenetic tree of Aquaporins (AQPs) from banana, Arabidopsis, poplar and rice. A neighbor-joining tree was constructed using ClustalX and MEGA5.0 software with 1000 bootstraps. Five subfamilies were indicated as PIP, SIP, NIP, TIP and X intrinsic proteins (XIPs). Green round, 47 AQP proteins from banana; red round, 35 AQP proteins from Arabidopsis; light blue, 33 AQP proteins from rice; dark blue, 56 AQP proteins from poplar.
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ijms-16-19728-f002: Phylogenetic tree of Aquaporins (AQPs) from banana, Arabidopsis, poplar and rice. A neighbor-joining tree was constructed using ClustalX and MEGA5.0 software with 1000 bootstraps. Five subfamilies were indicated as PIP, SIP, NIP, TIP and X intrinsic proteins (XIPs). Green round, 47 AQP proteins from banana; red round, 35 AQP proteins from Arabidopsis; light blue, 33 AQP proteins from rice; dark blue, 56 AQP proteins from poplar.

Mentions: To study the evolutionary relationships between AQP proteins from banana, Arabidopsis, poplar and rice, a neighbor-joining phylogenetic tree was created based on the alignments of their amino acid sequences (Figure 2, Table S6). The results showed that all identified AQPs from banana were clearly divided into four different subfamilies (PIP, TIP, NIP, and SIP), together with AQPs from Arabidopsis, poplar and rice. No banana AQP members were assigned into the XIP subfamily branched by poplar AQPs. According to the phylogenetic relationship, 18 MaAQPs were classified as PIPs, 17 as TIPs, 9 as NIPs, and 3 as SIPs. Furthermore, the PIPs, TIPs, NIPs, and SIPs were subdivided into two, five, four, and two subgroups (PIP1 and PIP2; TIP1, TIP2, TIP3, TIP4, and TIP5; NIP1, NIPP2, NIP3, and NIP4; SIP1 and SIP2), respectively. Overall, AQPs from banana had a closer relationship with those from rice than those from Arabidopsis and poplar, which is in line with current opinions in plant evolution. Moreover, BLASTP analysis further supports the phylogenetic classification of MaAQPs with each subfamily showing high identity with AQPs from other species (Table S6).


Genome-Wide Identification and Expression Analyses of Aquaporin Gene Family during Development and Abiotic Stress in Banana.

Hu W, Hou X, Huang C, Yan Y, Tie W, Ding Z, Wei Y, Liu J, Miao H, Lu Z, Li M, Xu B, Jin Z - Int J Mol Sci (2015)

Phylogenetic tree of Aquaporins (AQPs) from banana, Arabidopsis, poplar and rice. A neighbor-joining tree was constructed using ClustalX and MEGA5.0 software with 1000 bootstraps. Five subfamilies were indicated as PIP, SIP, NIP, TIP and X intrinsic proteins (XIPs). Green round, 47 AQP proteins from banana; red round, 35 AQP proteins from Arabidopsis; light blue, 33 AQP proteins from rice; dark blue, 56 AQP proteins from poplar.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581322&req=5

ijms-16-19728-f002: Phylogenetic tree of Aquaporins (AQPs) from banana, Arabidopsis, poplar and rice. A neighbor-joining tree was constructed using ClustalX and MEGA5.0 software with 1000 bootstraps. Five subfamilies were indicated as PIP, SIP, NIP, TIP and X intrinsic proteins (XIPs). Green round, 47 AQP proteins from banana; red round, 35 AQP proteins from Arabidopsis; light blue, 33 AQP proteins from rice; dark blue, 56 AQP proteins from poplar.
Mentions: To study the evolutionary relationships between AQP proteins from banana, Arabidopsis, poplar and rice, a neighbor-joining phylogenetic tree was created based on the alignments of their amino acid sequences (Figure 2, Table S6). The results showed that all identified AQPs from banana were clearly divided into four different subfamilies (PIP, TIP, NIP, and SIP), together with AQPs from Arabidopsis, poplar and rice. No banana AQP members were assigned into the XIP subfamily branched by poplar AQPs. According to the phylogenetic relationship, 18 MaAQPs were classified as PIPs, 17 as TIPs, 9 as NIPs, and 3 as SIPs. Furthermore, the PIPs, TIPs, NIPs, and SIPs were subdivided into two, five, four, and two subgroups (PIP1 and PIP2; TIP1, TIP2, TIP3, TIP4, and TIP5; NIP1, NIPP2, NIP3, and NIP4; SIP1 and SIP2), respectively. Overall, AQPs from banana had a closer relationship with those from rice than those from Arabidopsis and poplar, which is in line with current opinions in plant evolution. Moreover, BLASTP analysis further supports the phylogenetic classification of MaAQPs with each subfamily showing high identity with AQPs from other species (Table S6).

Bottom Line: Expression analysis of MaAQP genes during fruit development and postharvest ripening showed that some MaAQP genes exhibited high expression levels during these stages, indicating the involvement of MaAQP genes in banana fruit development and ripening.Additionally, some MaAQP genes showed strong induction after stress treatment and therefore, may represent potential candidates for improving banana resistance to abiotic stress.Taken together, this study identified some excellent tissue-specific, fruit development- and ripening-dependent, and abiotic stress-responsive candidate MaAQP genes, which could lay a solid foundation for genetic improvement of banana cultivars.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, China. huwei2010916@126.com.

ABSTRACT
Aquaporins (AQPs) function to selectively control the flow of water and other small molecules through biological membranes, playing crucial roles in various biological processes. However, little information is available on the AQP gene family in bananas. In this study, we identified 47 banana AQP genes based on the banana genome sequence. Evolutionary analysis of AQPs from banana, Arabidopsis, poplar, and rice indicated that banana AQPs (MaAQPs) were clustered into four subfamilies. Conserved motif analysis showed that all banana AQPs contained the typical AQP-like or major intrinsic protein (MIP) domain. Gene structure analysis suggested the majority of MaAQPs had two to four introns with a highly specific number and length for each subfamily. Expression analysis of MaAQP genes during fruit development and postharvest ripening showed that some MaAQP genes exhibited high expression levels during these stages, indicating the involvement of MaAQP genes in banana fruit development and ripening. Additionally, some MaAQP genes showed strong induction after stress treatment and therefore, may represent potential candidates for improving banana resistance to abiotic stress. Taken together, this study identified some excellent tissue-specific, fruit development- and ripening-dependent, and abiotic stress-responsive candidate MaAQP genes, which could lay a solid foundation for genetic improvement of banana cultivars.

No MeSH data available.