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Plasma-Derived Fibronectin Stimulates Chondrogenic Differentiation of Human Subchondral Cortico-Spongious Progenitor Cells in Late-Stage Osteoarthritis.

Jiang C, Ma P, Ma B, Wu Z, Qiu G, Su X, Xia Z, Ye Z, Wang Y - Int J Mol Sci (2015)

Bottom Line: Stimulating with Fn increased the expression of SOX-9, aggrecan, collagen II while decreased the formation of collagen I by immunochemical staining.Gene expression analysis showed similar results.These results suggest that plasma-derived Fn can increase the chondrogenic differentiation of SPCs isolated from late-stage OA and improve cartilage repair after microfracture.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China. jiangchao2189@sina.com.

ABSTRACT
Migration and chondrogenesis of human subchondral cortico-spongious progenitor cells (SPCs) are the key steps in the repair of microfracture-induced articular cartilage defects. The aim of this study was to evaluate the effect of human plasma-derived fibronectin (Fn) on the chondrogenic differentiation of SPCs, which was isolated from subchondrol cortico-spongious bone of late-stage osteoarthritis (OA) patients. SPCs were isolated and cultured for three passages. Stem cell surface antigens of SPCs were analyzed by flow cytometry. The osteogenic, chondrogenic and adipogenic differentiation potential were detected by histological staining. The chondrogenesis potential of SPCs with or without stimulation of either Fn or BMP-2 were studied by immunochemical staining and gene expression analysis. Cells isolated from subchondral bone presented to be positive for CD44, CD73, CD90, and CD166, and showed high capacity of osteogenic, adipogenic and chondrogenic differentiation, which suggested this cell population to be MSC-like cells. Stimulating with Fn increased the expression of SOX-9, aggrecan, collagen II while decreased the formation of collagen I by immunochemical staining. Gene expression analysis showed similar results. These results suggest that plasma-derived Fn can increase the chondrogenic differentiation of SPCs isolated from late-stage OA and improve cartilage repair after microfracture.

No MeSH data available.


Related in: MedlinePlus

Quantification of proliferation and migration of SPCs cultured under different conditions. (A) SPCs migrated through the pores of the membrane and adhered at the bottom site of the polycarbonate membrane. Fenestra was the pore of the polycarbonate membrane, and SPCs were stained in purple and were spindle-shaped (×200). (1) Negative control; (2) positive control; (3) BMP-2 group; (4) Fn group; (B) The migration index in different groups. * Compared with negative control, p < 0.05; (C) Cell proliferation under different conditions. * Compared with day 0, p < 0.05. The mean of each triplicate well is plotted and the error bars represent SD.
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ijms-16-19477-f006: Quantification of proliferation and migration of SPCs cultured under different conditions. (A) SPCs migrated through the pores of the membrane and adhered at the bottom site of the polycarbonate membrane. Fenestra was the pore of the polycarbonate membrane, and SPCs were stained in purple and were spindle-shaped (×200). (1) Negative control; (2) positive control; (3) BMP-2 group; (4) Fn group; (B) The migration index in different groups. * Compared with negative control, p < 0.05; (C) Cell proliferation under different conditions. * Compared with day 0, p < 0.05. The mean of each triplicate well is plotted and the error bars represent SD.

Mentions: Proliferation of SPCs had significantly increased since day 1 compared with day 0 in positive, BMP-2 and Fn groups (Figure 6C).


Plasma-Derived Fibronectin Stimulates Chondrogenic Differentiation of Human Subchondral Cortico-Spongious Progenitor Cells in Late-Stage Osteoarthritis.

Jiang C, Ma P, Ma B, Wu Z, Qiu G, Su X, Xia Z, Ye Z, Wang Y - Int J Mol Sci (2015)

Quantification of proliferation and migration of SPCs cultured under different conditions. (A) SPCs migrated through the pores of the membrane and adhered at the bottom site of the polycarbonate membrane. Fenestra was the pore of the polycarbonate membrane, and SPCs were stained in purple and were spindle-shaped (×200). (1) Negative control; (2) positive control; (3) BMP-2 group; (4) Fn group; (B) The migration index in different groups. * Compared with negative control, p < 0.05; (C) Cell proliferation under different conditions. * Compared with day 0, p < 0.05. The mean of each triplicate well is plotted and the error bars represent SD.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581308&req=5

ijms-16-19477-f006: Quantification of proliferation and migration of SPCs cultured under different conditions. (A) SPCs migrated through the pores of the membrane and adhered at the bottom site of the polycarbonate membrane. Fenestra was the pore of the polycarbonate membrane, and SPCs were stained in purple and were spindle-shaped (×200). (1) Negative control; (2) positive control; (3) BMP-2 group; (4) Fn group; (B) The migration index in different groups. * Compared with negative control, p < 0.05; (C) Cell proliferation under different conditions. * Compared with day 0, p < 0.05. The mean of each triplicate well is plotted and the error bars represent SD.
Mentions: Proliferation of SPCs had significantly increased since day 1 compared with day 0 in positive, BMP-2 and Fn groups (Figure 6C).

Bottom Line: Stimulating with Fn increased the expression of SOX-9, aggrecan, collagen II while decreased the formation of collagen I by immunochemical staining.Gene expression analysis showed similar results.These results suggest that plasma-derived Fn can increase the chondrogenic differentiation of SPCs isolated from late-stage OA and improve cartilage repair after microfracture.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China. jiangchao2189@sina.com.

ABSTRACT
Migration and chondrogenesis of human subchondral cortico-spongious progenitor cells (SPCs) are the key steps in the repair of microfracture-induced articular cartilage defects. The aim of this study was to evaluate the effect of human plasma-derived fibronectin (Fn) on the chondrogenic differentiation of SPCs, which was isolated from subchondrol cortico-spongious bone of late-stage osteoarthritis (OA) patients. SPCs were isolated and cultured for three passages. Stem cell surface antigens of SPCs were analyzed by flow cytometry. The osteogenic, chondrogenic and adipogenic differentiation potential were detected by histological staining. The chondrogenesis potential of SPCs with or without stimulation of either Fn or BMP-2 were studied by immunochemical staining and gene expression analysis. Cells isolated from subchondral bone presented to be positive for CD44, CD73, CD90, and CD166, and showed high capacity of osteogenic, adipogenic and chondrogenic differentiation, which suggested this cell population to be MSC-like cells. Stimulating with Fn increased the expression of SOX-9, aggrecan, collagen II while decreased the formation of collagen I by immunochemical staining. Gene expression analysis showed similar results. These results suggest that plasma-derived Fn can increase the chondrogenic differentiation of SPCs isolated from late-stage OA and improve cartilage repair after microfracture.

No MeSH data available.


Related in: MedlinePlus