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Protective Effects of Hong Shan Capsule against Lethal Total-Body Irradiation-Induced Damage in Wistar Rats.

Li J, Xu J, Xu W, Qi Y, Lu Y, Qiu L, Hu Z, Chu Z, Chai Y, Zhang J - Int J Mol Sci (2015)

Bottom Line: Gene expression profiles revealed the dramatic effect of HSC on alterations of gene expression caused by lethal TBI.Pretreatment with HSC prevented differential expression of 66% (1398 genes) of 2126 genes differentially expressed in response to TBI.Our data suggest that HSC has the potential to be used as an effective therapeutic or radio-protective agent to minimize irradiation damage.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, China. lijianzhong1234@hotmail.com.

ABSTRACT
Hong Shan Capsule (HSC), a crude drug of 11 medicinal herbs, was used in clinical practice for the treatment of radiation injuries in China. In this study, we investigated its protection in rats against acute lethal total-body irradiation (TBI). Pre-administration of HSC reduced the radiation sickness characteristics, while increasing the 30-day survival of the irradiated rats. Administration of HSC also reduced the radiation sickness characteristics and increased the 30-day survival of mice after exposure to lethal TBI. Ultrastructural observation illustrated that the pretreatment of rats with HSC significantly attenuated the TBI-induced morphological changes in the different organs of irradiated rats. Gene expression profiles revealed the dramatic effect of HSC on alterations of gene expression caused by lethal TBI. Pretreatment with HSC prevented differential expression of 66% (1398 genes) of 2126 genes differentially expressed in response to TBI. Pathway enrichment analysis indicated that these genes were mainly involved in a total of 32 pathways, such as pathways in cancer and the mitogen-activated protein kinase (MAPK) signaling pathway. Our analysis indicated that the pretreatment of rats with HSC modulated these pathways induced by lethal TBI, such as multiple MAPK pathways, suggesting that pretreatment with HSC might provide protective effects on lethal TBI mainly or partially through the modulation of these pathways. Our data suggest that HSC has the potential to be used as an effective therapeutic or radio-protective agent to minimize irradiation damage.

No MeSH data available.


Related in: MedlinePlus

Effect of HSC on radiation-induced tissues injury at one day after exposure to lethal TBI (6.5 Gy) using transmission electron microscopy. (A1–7) Representative photographs of non-irradiated rats; (B1–7) Representative photographs of 10 TBI rats. Cell swelling and large amounts of cytoplasmic vacuoles (arrows) were observed in the different tissues at one day after exposure to lethal TBI (6.5 Gy); (C1–7) Representative photographs of 10 irradiated rats pretreated with HSC (10 g/kg) once each day for three consecutive days before exposure to 6.5 Gy TBI. HSC pretreatment attenuated the radiation-induced tissue injury such as cytoplasmic vacuoles (arrows). (Uranyl acetate and lead citrate staining, 3000×).
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ijms-16-18938-f002: Effect of HSC on radiation-induced tissues injury at one day after exposure to lethal TBI (6.5 Gy) using transmission electron microscopy. (A1–7) Representative photographs of non-irradiated rats; (B1–7) Representative photographs of 10 TBI rats. Cell swelling and large amounts of cytoplasmic vacuoles (arrows) were observed in the different tissues at one day after exposure to lethal TBI (6.5 Gy); (C1–7) Representative photographs of 10 irradiated rats pretreated with HSC (10 g/kg) once each day for three consecutive days before exposure to 6.5 Gy TBI. HSC pretreatment attenuated the radiation-induced tissue injury such as cytoplasmic vacuoles (arrows). (Uranyl acetate and lead citrate staining, 3000×).

Mentions: To observe the possible protective or therapeutic effect of HSC on radiation-induced tissue injury in rats, transmission electron microscopy was performed. Rats received 10 g/kg/day of HSC or water for three days prior to radiation. Significant pathological changes occurred at one day after acute lethal TBI (6.5 Gy). Damage in the liver, spleen, small intestine, thymus, testis, cortex, and hippocampus such as cytoplasmic vacuolization, dilatation of the endoplasmic reticulum, and destruction of mitochondria, as well as damage to the cellular membrane, were observed (Figure 2B1–7). In addition, the morphological signs of apoptosis were frequently detected in the splenocytes. Apoptotic splenocytes showed the marginal condensation of chromatin onto the nuclear lamina (Figure 2B2). Vacuoles and mitochondrial swelling were decreased by HSC in the different organs (Figure 2C1–7). Cell apoptosis was also significantly attenuated by HSC in the spleen (Figure 2C2). These results illustrated that pretreatment of rats with HSC before exposure to acute lethal TBI significantly attenuated the radiation-induced morphological changes in the irradiated different organs, confirming the protective or therapeutic effect of HSC on radiation-induced tissue injury.


Protective Effects of Hong Shan Capsule against Lethal Total-Body Irradiation-Induced Damage in Wistar Rats.

Li J, Xu J, Xu W, Qi Y, Lu Y, Qiu L, Hu Z, Chu Z, Chai Y, Zhang J - Int J Mol Sci (2015)

Effect of HSC on radiation-induced tissues injury at one day after exposure to lethal TBI (6.5 Gy) using transmission electron microscopy. (A1–7) Representative photographs of non-irradiated rats; (B1–7) Representative photographs of 10 TBI rats. Cell swelling and large amounts of cytoplasmic vacuoles (arrows) were observed in the different tissues at one day after exposure to lethal TBI (6.5 Gy); (C1–7) Representative photographs of 10 irradiated rats pretreated with HSC (10 g/kg) once each day for three consecutive days before exposure to 6.5 Gy TBI. HSC pretreatment attenuated the radiation-induced tissue injury such as cytoplasmic vacuoles (arrows). (Uranyl acetate and lead citrate staining, 3000×).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581280&req=5

ijms-16-18938-f002: Effect of HSC on radiation-induced tissues injury at one day after exposure to lethal TBI (6.5 Gy) using transmission electron microscopy. (A1–7) Representative photographs of non-irradiated rats; (B1–7) Representative photographs of 10 TBI rats. Cell swelling and large amounts of cytoplasmic vacuoles (arrows) were observed in the different tissues at one day after exposure to lethal TBI (6.5 Gy); (C1–7) Representative photographs of 10 irradiated rats pretreated with HSC (10 g/kg) once each day for three consecutive days before exposure to 6.5 Gy TBI. HSC pretreatment attenuated the radiation-induced tissue injury such as cytoplasmic vacuoles (arrows). (Uranyl acetate and lead citrate staining, 3000×).
Mentions: To observe the possible protective or therapeutic effect of HSC on radiation-induced tissue injury in rats, transmission electron microscopy was performed. Rats received 10 g/kg/day of HSC or water for three days prior to radiation. Significant pathological changes occurred at one day after acute lethal TBI (6.5 Gy). Damage in the liver, spleen, small intestine, thymus, testis, cortex, and hippocampus such as cytoplasmic vacuolization, dilatation of the endoplasmic reticulum, and destruction of mitochondria, as well as damage to the cellular membrane, were observed (Figure 2B1–7). In addition, the morphological signs of apoptosis were frequently detected in the splenocytes. Apoptotic splenocytes showed the marginal condensation of chromatin onto the nuclear lamina (Figure 2B2). Vacuoles and mitochondrial swelling were decreased by HSC in the different organs (Figure 2C1–7). Cell apoptosis was also significantly attenuated by HSC in the spleen (Figure 2C2). These results illustrated that pretreatment of rats with HSC before exposure to acute lethal TBI significantly attenuated the radiation-induced morphological changes in the irradiated different organs, confirming the protective or therapeutic effect of HSC on radiation-induced tissue injury.

Bottom Line: Gene expression profiles revealed the dramatic effect of HSC on alterations of gene expression caused by lethal TBI.Pretreatment with HSC prevented differential expression of 66% (1398 genes) of 2126 genes differentially expressed in response to TBI.Our data suggest that HSC has the potential to be used as an effective therapeutic or radio-protective agent to minimize irradiation damage.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, China. lijianzhong1234@hotmail.com.

ABSTRACT
Hong Shan Capsule (HSC), a crude drug of 11 medicinal herbs, was used in clinical practice for the treatment of radiation injuries in China. In this study, we investigated its protection in rats against acute lethal total-body irradiation (TBI). Pre-administration of HSC reduced the radiation sickness characteristics, while increasing the 30-day survival of the irradiated rats. Administration of HSC also reduced the radiation sickness characteristics and increased the 30-day survival of mice after exposure to lethal TBI. Ultrastructural observation illustrated that the pretreatment of rats with HSC significantly attenuated the TBI-induced morphological changes in the different organs of irradiated rats. Gene expression profiles revealed the dramatic effect of HSC on alterations of gene expression caused by lethal TBI. Pretreatment with HSC prevented differential expression of 66% (1398 genes) of 2126 genes differentially expressed in response to TBI. Pathway enrichment analysis indicated that these genes were mainly involved in a total of 32 pathways, such as pathways in cancer and the mitogen-activated protein kinase (MAPK) signaling pathway. Our analysis indicated that the pretreatment of rats with HSC modulated these pathways induced by lethal TBI, such as multiple MAPK pathways, suggesting that pretreatment with HSC might provide protective effects on lethal TBI mainly or partially through the modulation of these pathways. Our data suggest that HSC has the potential to be used as an effective therapeutic or radio-protective agent to minimize irradiation damage.

No MeSH data available.


Related in: MedlinePlus