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Differentiation Effects of Platelet-Rich Plasma Concentrations on Synovial Fluid Mesenchymal Stem Cells from Pigs Cultivated in Alginate Complex Hydrogel.

Tang HC, Chen WC, Chiang CW, Chen LY, Chang YC, Chen CH - Int J Mol Sci (2015)

Bottom Line: The effects of various PRP concentrations (0%, 20% and 50% PRP) on differentiation were evaluated using the SF-MSCs-alginate system, such as gene expression and DNA proliferation.A 50% PRP concentration yielded better differentiation than the 20% PRP concentration.Both PRP and SF-MSCs could be feasibly used in regenerative medicine and orthopedic surgeries.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Chang Gung Memorial Hospital, Keelung 204, Taiwan. tanghaoche@gmail.com.

ABSTRACT
This article studied the effects of platelet-rich plasma (PRP) on the potential of synovial fluid mesenchymal stem cells (SF-MSCs) to differentiate. The PRP and SF-MSCs were obtained from the blood and knees of pigs, respectively. The identification of SF-MSCs and their ability to differentiate were studied by histological and surface epitopes, respectively. The SF-MSCs can undergo trilineage mesenchymal differentiation under osteogenic, chondrogenic, and adipocyte induction. The effects of various PRP concentrations (0%, 20% and 50% PRP) on differentiation were evaluated using the SF-MSCs-alginate system, such as gene expression and DNA proliferation. A 50% PRP concentration yielded better differentiation than the 20% PRP concentration. PRP favored the chondrogenesis of SF-MSCs over their osteogenesis in a manner that depended on the ratios of type II collagen/type I collagen and aggrecan/osteopontin. Eventually, PRP promoted the proliferation of SF-MSCs and induced chondrogenic differentiation of SF-MSCs in vitro. Both PRP and SF-MSCs could be feasibly used in regenerative medicine and orthopedic surgeries.

No MeSH data available.


The morphology of PRP-SF-MSCs-alginate complex with (A) 0% PRP and (B) 20% or 50%. Scale unit: 1 cm.
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ijms-16-18507-f007: The morphology of PRP-SF-MSCs-alginate complex with (A) 0% PRP and (B) 20% or 50%. Scale unit: 1 cm.

Mentions: Low-viscosity sodium salt alginic acid (Sigma) was used in these studies. This alginate is derived from Macrocystitis pyrifera, has a mannuronic acid/guluronic acid ratio of 1.67 and a molecular weight of around 50,000 Dalton. One milliliter of alginate (10%) was dissolved in 9 mL of PBS and sterilized for 30 min at 121 °C in an autoclave, then cooled at room temperature. PRP was added to the 1% alginate-PBS solution to form PRP-alginate solutions with 20% and 50% concentration of PRP, respectively, in a laminar flow hood. The SF-MSCs were treated with 0.5% trypsin before four passages, and a 5 × 104 cells/mL density was prepared in the PRP-alginate solution. In addition, SF-MSCs were added into the alginate-PBS solution without PRP to make the control group. Then the PRP-SF-MSCs-alginate solution was dropped into a 0.1 M calcium chloride solution. After 2 min of reaction, the PSA hydrogel was obtained (Figure 7A,B). Then, the PSA hydrogels with different PRP concentration (0%, 20%, and 50%) were placed into 24-well culture plates, and 1 mL of DMEM medium (containing 10% FBS) was added to each well. The culture medium was replaced every two days.


Differentiation Effects of Platelet-Rich Plasma Concentrations on Synovial Fluid Mesenchymal Stem Cells from Pigs Cultivated in Alginate Complex Hydrogel.

Tang HC, Chen WC, Chiang CW, Chen LY, Chang YC, Chen CH - Int J Mol Sci (2015)

The morphology of PRP-SF-MSCs-alginate complex with (A) 0% PRP and (B) 20% or 50%. Scale unit: 1 cm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581257&req=5

ijms-16-18507-f007: The morphology of PRP-SF-MSCs-alginate complex with (A) 0% PRP and (B) 20% or 50%. Scale unit: 1 cm.
Mentions: Low-viscosity sodium salt alginic acid (Sigma) was used in these studies. This alginate is derived from Macrocystitis pyrifera, has a mannuronic acid/guluronic acid ratio of 1.67 and a molecular weight of around 50,000 Dalton. One milliliter of alginate (10%) was dissolved in 9 mL of PBS and sterilized for 30 min at 121 °C in an autoclave, then cooled at room temperature. PRP was added to the 1% alginate-PBS solution to form PRP-alginate solutions with 20% and 50% concentration of PRP, respectively, in a laminar flow hood. The SF-MSCs were treated with 0.5% trypsin before four passages, and a 5 × 104 cells/mL density was prepared in the PRP-alginate solution. In addition, SF-MSCs were added into the alginate-PBS solution without PRP to make the control group. Then the PRP-SF-MSCs-alginate solution was dropped into a 0.1 M calcium chloride solution. After 2 min of reaction, the PSA hydrogel was obtained (Figure 7A,B). Then, the PSA hydrogels with different PRP concentration (0%, 20%, and 50%) were placed into 24-well culture plates, and 1 mL of DMEM medium (containing 10% FBS) was added to each well. The culture medium was replaced every two days.

Bottom Line: The effects of various PRP concentrations (0%, 20% and 50% PRP) on differentiation were evaluated using the SF-MSCs-alginate system, such as gene expression and DNA proliferation.A 50% PRP concentration yielded better differentiation than the 20% PRP concentration.Both PRP and SF-MSCs could be feasibly used in regenerative medicine and orthopedic surgeries.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Chang Gung Memorial Hospital, Keelung 204, Taiwan. tanghaoche@gmail.com.

ABSTRACT
This article studied the effects of platelet-rich plasma (PRP) on the potential of synovial fluid mesenchymal stem cells (SF-MSCs) to differentiate. The PRP and SF-MSCs were obtained from the blood and knees of pigs, respectively. The identification of SF-MSCs and their ability to differentiate were studied by histological and surface epitopes, respectively. The SF-MSCs can undergo trilineage mesenchymal differentiation under osteogenic, chondrogenic, and adipocyte induction. The effects of various PRP concentrations (0%, 20% and 50% PRP) on differentiation were evaluated using the SF-MSCs-alginate system, such as gene expression and DNA proliferation. A 50% PRP concentration yielded better differentiation than the 20% PRP concentration. PRP favored the chondrogenesis of SF-MSCs over their osteogenesis in a manner that depended on the ratios of type II collagen/type I collagen and aggrecan/osteopontin. Eventually, PRP promoted the proliferation of SF-MSCs and induced chondrogenic differentiation of SF-MSCs in vitro. Both PRP and SF-MSCs could be feasibly used in regenerative medicine and orthopedic surgeries.

No MeSH data available.