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Differentiation Effects of Platelet-Rich Plasma Concentrations on Synovial Fluid Mesenchymal Stem Cells from Pigs Cultivated in Alginate Complex Hydrogel.

Tang HC, Chen WC, Chiang CW, Chen LY, Chang YC, Chen CH - Int J Mol Sci (2015)

Bottom Line: The effects of various PRP concentrations (0%, 20% and 50% PRP) on differentiation were evaluated using the SF-MSCs-alginate system, such as gene expression and DNA proliferation.A 50% PRP concentration yielded better differentiation than the 20% PRP concentration.Both PRP and SF-MSCs could be feasibly used in regenerative medicine and orthopedic surgeries.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Chang Gung Memorial Hospital, Keelung 204, Taiwan. tanghaoche@gmail.com.

ABSTRACT
This article studied the effects of platelet-rich plasma (PRP) on the potential of synovial fluid mesenchymal stem cells (SF-MSCs) to differentiate. The PRP and SF-MSCs were obtained from the blood and knees of pigs, respectively. The identification of SF-MSCs and their ability to differentiate were studied by histological and surface epitopes, respectively. The SF-MSCs can undergo trilineage mesenchymal differentiation under osteogenic, chondrogenic, and adipocyte induction. The effects of various PRP concentrations (0%, 20% and 50% PRP) on differentiation were evaluated using the SF-MSCs-alginate system, such as gene expression and DNA proliferation. A 50% PRP concentration yielded better differentiation than the 20% PRP concentration. PRP favored the chondrogenesis of SF-MSCs over their osteogenesis in a manner that depended on the ratios of type II collagen/type I collagen and aggrecan/osteopontin. Eventually, PRP promoted the proliferation of SF-MSCs and induced chondrogenic differentiation of SF-MSCs in vitro. Both PRP and SF-MSCs could be feasibly used in regenerative medicine and orthopedic surgeries.

No MeSH data available.


(A) Cell colonies of synovial fluid mesenchymal stem cells (SF-MSCs) after a 10-day cultivation; (B) The morphology of SF-MSCs. Three SF-MSCs samples were performed for this experiment.
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ijms-16-18507-f001: (A) Cell colonies of synovial fluid mesenchymal stem cells (SF-MSCs) after a 10-day cultivation; (B) The morphology of SF-MSCs. Three SF-MSCs samples were performed for this experiment.

Mentions: Spindle-shaped SF-MSCs of pigs were observed using a light microscope; their phenotype is similar to that of human SF-MSCs [10]. Only a few approximate colonies are shown in passage 0 after 10 days of cultivation (Figure 1A). The SF-MSCs maintained their spindle shape for four passages (Figure 1B).


Differentiation Effects of Platelet-Rich Plasma Concentrations on Synovial Fluid Mesenchymal Stem Cells from Pigs Cultivated in Alginate Complex Hydrogel.

Tang HC, Chen WC, Chiang CW, Chen LY, Chang YC, Chen CH - Int J Mol Sci (2015)

(A) Cell colonies of synovial fluid mesenchymal stem cells (SF-MSCs) after a 10-day cultivation; (B) The morphology of SF-MSCs. Three SF-MSCs samples were performed for this experiment.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581257&req=5

ijms-16-18507-f001: (A) Cell colonies of synovial fluid mesenchymal stem cells (SF-MSCs) after a 10-day cultivation; (B) The morphology of SF-MSCs. Three SF-MSCs samples were performed for this experiment.
Mentions: Spindle-shaped SF-MSCs of pigs were observed using a light microscope; their phenotype is similar to that of human SF-MSCs [10]. Only a few approximate colonies are shown in passage 0 after 10 days of cultivation (Figure 1A). The SF-MSCs maintained their spindle shape for four passages (Figure 1B).

Bottom Line: The effects of various PRP concentrations (0%, 20% and 50% PRP) on differentiation were evaluated using the SF-MSCs-alginate system, such as gene expression and DNA proliferation.A 50% PRP concentration yielded better differentiation than the 20% PRP concentration.Both PRP and SF-MSCs could be feasibly used in regenerative medicine and orthopedic surgeries.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Chang Gung Memorial Hospital, Keelung 204, Taiwan. tanghaoche@gmail.com.

ABSTRACT
This article studied the effects of platelet-rich plasma (PRP) on the potential of synovial fluid mesenchymal stem cells (SF-MSCs) to differentiate. The PRP and SF-MSCs were obtained from the blood and knees of pigs, respectively. The identification of SF-MSCs and their ability to differentiate were studied by histological and surface epitopes, respectively. The SF-MSCs can undergo trilineage mesenchymal differentiation under osteogenic, chondrogenic, and adipocyte induction. The effects of various PRP concentrations (0%, 20% and 50% PRP) on differentiation were evaluated using the SF-MSCs-alginate system, such as gene expression and DNA proliferation. A 50% PRP concentration yielded better differentiation than the 20% PRP concentration. PRP favored the chondrogenesis of SF-MSCs over their osteogenesis in a manner that depended on the ratios of type II collagen/type I collagen and aggrecan/osteopontin. Eventually, PRP promoted the proliferation of SF-MSCs and induced chondrogenic differentiation of SF-MSCs in vitro. Both PRP and SF-MSCs could be feasibly used in regenerative medicine and orthopedic surgeries.

No MeSH data available.