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The Essential Role of Vitellogenin Receptor in Ovary Development and Vitellogenin Uptake in Bactrocera dorsalis (Hendel).

Cong L, Yang WJ, Jiang XZ, Niu JZ, Shen GM, Ran C, Wang JJ - Int J Mol Sci (2015)

Bottom Line: The developmental expression patterns showed that the signal of BdVgR was detectable in very beginning of adult stage, and positively correlated with the growth rate of ovaries and the expression levels of its ligands.In addition, we also demonstrated that the expression level of BdVgR, and ovary development were significantly suppressed after being injected with BdVgR-targeted dsRNA.Together, all of these results indicated that BdVgR was critical for yolk protein absorption and ovary maturation in B. dorsalis, playing a vital role in female reproduction.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China. ranchun@cric.cn.

ABSTRACT
The vitellogenin receptor (VgR) functions as an essential component in uptaking and transporting vitellogenin (Vg) in female adults, which is involved in ovary development and oviposition. This study aimed to clarify the molecular characteristics and function of VgR in the oriental fruit fly Bactrocera dorsalis (Hendel). Here, we identified the full-length of BdVgR (GenBank Accession No. JX469118), encoding a 1925 residue (aa) protein with a 214.72 kDa molecular mass and several typical motifs of low-density lipoprotein receptor superfamily (LDLR). Phylogenic analysis suggested that BdVgR was evolutionary conserved with other Dipteran VgRs. The expression of BdVgR was exclusively detected in the ovaries rather than head, thorax or other tissues. The developmental expression patterns showed that the signal of BdVgR was detectable in very beginning of adult stage, and positively correlated with the growth rate of ovaries and the expression levels of its ligands. In addition, we also demonstrated that the expression level of BdVgR, and ovary development were significantly suppressed after being injected with BdVgR-targeted dsRNA. Together, all of these results indicated that BdVgR was critical for yolk protein absorption and ovary maturation in B. dorsalis, playing a vital role in female reproduction.

No MeSH data available.


Related in: MedlinePlus

The growth of ovary and temporal-expression patterns of BdVgR, Bdyp1 and Bdyp2: (A) the developmental status of ovaries; (B) the temporal-expression pattern of BdVgR; (C) the temporal-expression pattern of Bdyp1; (D) the temporal-expression pattern of Bdyp2. The bars represented the mean ± SE. Different letters indicated significant difference with ANOVA (Least Significant Difference, LSD, p < 0.05).
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ijms-16-18368-f004: The growth of ovary and temporal-expression patterns of BdVgR, Bdyp1 and Bdyp2: (A) the developmental status of ovaries; (B) the temporal-expression pattern of BdVgR; (C) the temporal-expression pattern of Bdyp1; (D) the temporal-expression pattern of Bdyp2. The bars represented the mean ± SE. Different letters indicated significant difference with ANOVA (Least Significant Difference, LSD, p < 0.05).

Mentions: To evaluate the potential relationship between the development of ovary and BdVgR, the diameters of ovaries and the expression profiles of BdVgR were monitored throughout the different ages of female adults, in addition with its ligands, Bdyp1 and Bdyp2 (Figure 4). As demonstrated in Figure 4A, no obvious enlargements were detected during the first four days. However, a dramatic enlargement was observed on the seventh day, kept increasing in the following days, and finally reached the maximum size on the 16th day (2.146 ± 0.0354 mm), which was 4.13 times larger than that on the first day (0.522 ± 0.0064 mm). The developmental expression patterns showed that the mRNA of BdVgR, Bdyp1 and Bdyp2 were detectable throughout all selected time points, but extremely low in the very beginning of the adult stage (Figure 4B–D). Synchronous with the dramatic change in ovarian development, the expression level of BdVgR was also significantly increased to its summit on the seventh day, which was 102.31 times of that in the first day adults (Figure 4B). However, there was a turning point on the tenth day. The expression level of BdVgR quickly down-regulated, decreasing to 21.85% compared to the expression level of the seventh day, but finally recovered to a certain high level during the following days. Interestingly, Bdyp1 and Bdyp2 had similar transcriptional patterns, as they started to increase steeply in the seventh day and reached their maximum levels in the tenth day. However, the highest expression level of BdVgR appeared on the seventh day, three days earlier than that in Bdyp1 and Bdyp2 (Figure 4C,D).


The Essential Role of Vitellogenin Receptor in Ovary Development and Vitellogenin Uptake in Bactrocera dorsalis (Hendel).

Cong L, Yang WJ, Jiang XZ, Niu JZ, Shen GM, Ran C, Wang JJ - Int J Mol Sci (2015)

The growth of ovary and temporal-expression patterns of BdVgR, Bdyp1 and Bdyp2: (A) the developmental status of ovaries; (B) the temporal-expression pattern of BdVgR; (C) the temporal-expression pattern of Bdyp1; (D) the temporal-expression pattern of Bdyp2. The bars represented the mean ± SE. Different letters indicated significant difference with ANOVA (Least Significant Difference, LSD, p < 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581250&req=5

ijms-16-18368-f004: The growth of ovary and temporal-expression patterns of BdVgR, Bdyp1 and Bdyp2: (A) the developmental status of ovaries; (B) the temporal-expression pattern of BdVgR; (C) the temporal-expression pattern of Bdyp1; (D) the temporal-expression pattern of Bdyp2. The bars represented the mean ± SE. Different letters indicated significant difference with ANOVA (Least Significant Difference, LSD, p < 0.05).
Mentions: To evaluate the potential relationship between the development of ovary and BdVgR, the diameters of ovaries and the expression profiles of BdVgR were monitored throughout the different ages of female adults, in addition with its ligands, Bdyp1 and Bdyp2 (Figure 4). As demonstrated in Figure 4A, no obvious enlargements were detected during the first four days. However, a dramatic enlargement was observed on the seventh day, kept increasing in the following days, and finally reached the maximum size on the 16th day (2.146 ± 0.0354 mm), which was 4.13 times larger than that on the first day (0.522 ± 0.0064 mm). The developmental expression patterns showed that the mRNA of BdVgR, Bdyp1 and Bdyp2 were detectable throughout all selected time points, but extremely low in the very beginning of the adult stage (Figure 4B–D). Synchronous with the dramatic change in ovarian development, the expression level of BdVgR was also significantly increased to its summit on the seventh day, which was 102.31 times of that in the first day adults (Figure 4B). However, there was a turning point on the tenth day. The expression level of BdVgR quickly down-regulated, decreasing to 21.85% compared to the expression level of the seventh day, but finally recovered to a certain high level during the following days. Interestingly, Bdyp1 and Bdyp2 had similar transcriptional patterns, as they started to increase steeply in the seventh day and reached their maximum levels in the tenth day. However, the highest expression level of BdVgR appeared on the seventh day, three days earlier than that in Bdyp1 and Bdyp2 (Figure 4C,D).

Bottom Line: The developmental expression patterns showed that the signal of BdVgR was detectable in very beginning of adult stage, and positively correlated with the growth rate of ovaries and the expression levels of its ligands.In addition, we also demonstrated that the expression level of BdVgR, and ovary development were significantly suppressed after being injected with BdVgR-targeted dsRNA.Together, all of these results indicated that BdVgR was critical for yolk protein absorption and ovary maturation in B. dorsalis, playing a vital role in female reproduction.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China. ranchun@cric.cn.

ABSTRACT
The vitellogenin receptor (VgR) functions as an essential component in uptaking and transporting vitellogenin (Vg) in female adults, which is involved in ovary development and oviposition. This study aimed to clarify the molecular characteristics and function of VgR in the oriental fruit fly Bactrocera dorsalis (Hendel). Here, we identified the full-length of BdVgR (GenBank Accession No. JX469118), encoding a 1925 residue (aa) protein with a 214.72 kDa molecular mass and several typical motifs of low-density lipoprotein receptor superfamily (LDLR). Phylogenic analysis suggested that BdVgR was evolutionary conserved with other Dipteran VgRs. The expression of BdVgR was exclusively detected in the ovaries rather than head, thorax or other tissues. The developmental expression patterns showed that the signal of BdVgR was detectable in very beginning of adult stage, and positively correlated with the growth rate of ovaries and the expression levels of its ligands. In addition, we also demonstrated that the expression level of BdVgR, and ovary development were significantly suppressed after being injected with BdVgR-targeted dsRNA. Together, all of these results indicated that BdVgR was critical for yolk protein absorption and ovary maturation in B. dorsalis, playing a vital role in female reproduction.

No MeSH data available.


Related in: MedlinePlus