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Annexin A1 Is Increased in the Plasma of Preeclamptic Women.

Perucci LO, Carneiro FS, Ferreira CN, Sugimoto MA, Soriani FM, Martins GG, Lima KM, Guimarães FL, Teixeira AL, Dusse LM, Gomes KB, Sousa LP - PLoS ONE (2015)

Bottom Line: Preeclampsia (PE) is a pregnancy disease associated with exacerbated inflammatory response.No significant difference was found for AnxA1 and us-CRP, comparing late PE and normotensive pregnant women with GA ≥ 34 weeks.Our data show that increased AnxA1 levels were associated with a systemic inflammatory phenotype in PE, suggesting AnxA1 deregulation in PE pathogenesis.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; Programa de Pós-Graduação em Análises Clínicas e Toxicológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

ABSTRACT

Background: Preeclampsia (PE) is a pregnancy disease associated with exacerbated inflammatory response. Annexin A1 (AnxA1) is a glucocorticoid-regulated protein endowed with anti-inflammatory and proresolving properties that has been much studied in various animal models of inflammation but poorly studied in the context of human inflammatory diseases. The main objective of this study was to measure AnxA1 levels in PE women and to compare those levels in normotensive pregnant and non-pregnant women. We evaluated the association among AnxA1, ultrasensitive C reactive protein (us-CRP) and soluble tumor necrosis factor alpha receptor type 1 (sTNF-R1) plasma levels of the study participants.

Methods: This study included 40 non-pregnant, 38 normotensive pregnant and 51 PE women. PE women were stratified in early (N = 23) and late (N = 28) subgroups, according to gestational age (GA) at onset of clinical symptoms. Protein AnxA1 and us-CRP plasma levels were determined by ELISA and immunoturbidimetric assays, respectively. Transcript levels of AnxA1 in peripheral blood mononuclear cells (PBMC) were measured by real time RT-PCR.

Results: Increased levels of AnxA1 coincided with higher us-CRP levels in the plasma of PE women. Pregnant women with early PE had higher levels of AnxA1 and us-CRP than normotensive pregnant women with GA <34 weeks. No significant difference was found for AnxA1 and us-CRP, comparing late PE and normotensive pregnant women with GA ≥ 34 weeks. AnxA1 mRNA levels in PBMC were similar among the studied groups. AnxA1 was positively correlated with sTNF-R1, but not with us-CRP.

Conclusions: Our data show that increased AnxA1 levels were associated with a systemic inflammatory phenotype in PE, suggesting AnxA1 deregulation in PE pathogenesis. However, more studies are needed to clarify the role of AnxA1 and other proresolving molecules in the context of the systemic inflammatory response in this intriguing disease.

No MeSH data available.


Related in: MedlinePlus

us-CRP plasma levels in non-pregnant, normotensive pregnant and PE women.NP (non-pregnant women), Norm (normotensive pregnant women), PE (preeclamptic women). Horizontal bars represent median values for us-CRP (milligrams/liter). ***P<0.001. Plasma levels of us-CRP were higher in normotensive pregnant women compared to non-pregnant women and in PE women than in non-pregnant women. No significant difference was detected between PE and normotensive pregnant women.
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pone.0138475.g004: us-CRP plasma levels in non-pregnant, normotensive pregnant and PE women.NP (non-pregnant women), Norm (normotensive pregnant women), PE (preeclamptic women). Horizontal bars represent median values for us-CRP (milligrams/liter). ***P<0.001. Plasma levels of us-CRP were higher in normotensive pregnant women compared to non-pregnant women and in PE women than in non-pregnant women. No significant difference was detected between PE and normotensive pregnant women.

Mentions: Us-CRP plasma levels were higher in PE women [5.8 (3.6–15.0)mg/L] than in non-pregnant women [0.9 (0.2–2.4)mg/L] (P<0.001) and in normotensive pregnant women [3.9 (2.8–6.4)mg/L] compared to non-pregnant women (P<0.001). No significant difference was found in us-CRP levels between PE women and in normotensive pregnant women, without stratifying the groups according to gestational age (Fig 4).


Annexin A1 Is Increased in the Plasma of Preeclamptic Women.

Perucci LO, Carneiro FS, Ferreira CN, Sugimoto MA, Soriani FM, Martins GG, Lima KM, Guimarães FL, Teixeira AL, Dusse LM, Gomes KB, Sousa LP - PLoS ONE (2015)

us-CRP plasma levels in non-pregnant, normotensive pregnant and PE women.NP (non-pregnant women), Norm (normotensive pregnant women), PE (preeclamptic women). Horizontal bars represent median values for us-CRP (milligrams/liter). ***P<0.001. Plasma levels of us-CRP were higher in normotensive pregnant women compared to non-pregnant women and in PE women than in non-pregnant women. No significant difference was detected between PE and normotensive pregnant women.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4580581&req=5

pone.0138475.g004: us-CRP plasma levels in non-pregnant, normotensive pregnant and PE women.NP (non-pregnant women), Norm (normotensive pregnant women), PE (preeclamptic women). Horizontal bars represent median values for us-CRP (milligrams/liter). ***P<0.001. Plasma levels of us-CRP were higher in normotensive pregnant women compared to non-pregnant women and in PE women than in non-pregnant women. No significant difference was detected between PE and normotensive pregnant women.
Mentions: Us-CRP plasma levels were higher in PE women [5.8 (3.6–15.0)mg/L] than in non-pregnant women [0.9 (0.2–2.4)mg/L] (P<0.001) and in normotensive pregnant women [3.9 (2.8–6.4)mg/L] compared to non-pregnant women (P<0.001). No significant difference was found in us-CRP levels between PE women and in normotensive pregnant women, without stratifying the groups according to gestational age (Fig 4).

Bottom Line: Preeclampsia (PE) is a pregnancy disease associated with exacerbated inflammatory response.No significant difference was found for AnxA1 and us-CRP, comparing late PE and normotensive pregnant women with GA ≥ 34 weeks.Our data show that increased AnxA1 levels were associated with a systemic inflammatory phenotype in PE, suggesting AnxA1 deregulation in PE pathogenesis.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; Programa de Pós-Graduação em Análises Clínicas e Toxicológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

ABSTRACT

Background: Preeclampsia (PE) is a pregnancy disease associated with exacerbated inflammatory response. Annexin A1 (AnxA1) is a glucocorticoid-regulated protein endowed with anti-inflammatory and proresolving properties that has been much studied in various animal models of inflammation but poorly studied in the context of human inflammatory diseases. The main objective of this study was to measure AnxA1 levels in PE women and to compare those levels in normotensive pregnant and non-pregnant women. We evaluated the association among AnxA1, ultrasensitive C reactive protein (us-CRP) and soluble tumor necrosis factor alpha receptor type 1 (sTNF-R1) plasma levels of the study participants.

Methods: This study included 40 non-pregnant, 38 normotensive pregnant and 51 PE women. PE women were stratified in early (N = 23) and late (N = 28) subgroups, according to gestational age (GA) at onset of clinical symptoms. Protein AnxA1 and us-CRP plasma levels were determined by ELISA and immunoturbidimetric assays, respectively. Transcript levels of AnxA1 in peripheral blood mononuclear cells (PBMC) were measured by real time RT-PCR.

Results: Increased levels of AnxA1 coincided with higher us-CRP levels in the plasma of PE women. Pregnant women with early PE had higher levels of AnxA1 and us-CRP than normotensive pregnant women with GA <34 weeks. No significant difference was found for AnxA1 and us-CRP, comparing late PE and normotensive pregnant women with GA ≥ 34 weeks. AnxA1 mRNA levels in PBMC were similar among the studied groups. AnxA1 was positively correlated with sTNF-R1, but not with us-CRP.

Conclusions: Our data show that increased AnxA1 levels were associated with a systemic inflammatory phenotype in PE, suggesting AnxA1 deregulation in PE pathogenesis. However, more studies are needed to clarify the role of AnxA1 and other proresolving molecules in the context of the systemic inflammatory response in this intriguing disease.

No MeSH data available.


Related in: MedlinePlus