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Quantum Dots Encapsulated with Canine Parvovirus-Like Particles Improving the Cellular Targeted Labeling.

Yan D, Wang B, Sun S, Feng X, Jin Y, Yao X, Cao S, Guo H - PLoS ONE (2015)

Bottom Line: When incubated with different cell lines, CPV-VLPs-QDs significantly reduced the cytotoxicity of QDs and selectively labeled the cells with high-level transferrin receptors.Therefore, CPV-VLPs can be used as carriers to facilitate the targeted delivery of encapsulated nanomaterials into cells via receptor-mediated pathways.This study confirmed that CPV-VLPs can significantly promote the biocompatibility of nanomaterials and could expand the application of CPV-VLPs in biological medicine.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Veterinary Etiological Biology and National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu 730046, China.

ABSTRACT
Quantum dots (QDs) have a promising prospect in live-cell imaging and sensing because of unique fluorescence features. QDs aroused significant interest in the bio-imaging field through integrating the fluorescence properties of QDs and the delivery function of biomaterial. The natural tropism of Canine Parvovirus (CPV) to the transferrin receptor can target specific cells to increase the targeting ability of QDs in cell imaging. CPV virus-like particles (VLPs) from the expression of the CPV-VP2 capsid protein in a prokaryotic expression system were examined to encapsulate the QDs and deliver to cells with an expressed transferrin receptor. CPV-VLPs were used to encapsulate QDs that were modified using 3-mercaptopropionic acid. Gel electrophoresis, fluorescence spectrum, particle size, and transmission electron microscopy verified the conformation of a complex, in which QDs were encapsulated in CPV-VLPs (CPV-VLPs-QDs). When incubated with different cell lines, CPV-VLPs-QDs significantly reduced the cytotoxicity of QDs and selectively labeled the cells with high-level transferrin receptors. Cell-targeted labeling was achieved by utilizing the specific binding between the CPV capsid protein VP2 of VLPs and cellular receptors. CPV-VLPs-QDs, which can mimic the native CPV infection, can recognize and attach to the transferrin receptors on cellular membrane. Therefore, CPV-VLPs can be used as carriers to facilitate the targeted delivery of encapsulated nanomaterials into cells via receptor-mediated pathways. This study confirmed that CPV-VLPs can significantly promote the biocompatibility of nanomaterials and could expand the application of CPV-VLPs in biological medicine.

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Related in: MedlinePlus

Optimization of QDs encapsulation using CPV-VLPs.(a) Particle size of CPV-VLPs-QDs under different CPV-VLPs/QDs ratios. (b)Absorbance of complex at different CPV-VLPs/QDs ratios.
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pone.0138883.g004: Optimization of QDs encapsulation using CPV-VLPs.(a) Particle size of CPV-VLPs-QDs under different CPV-VLPs/QDs ratios. (b)Absorbance of complex at different CPV-VLPs/QDs ratios.

Mentions: To ensure that VLPs encapsulated the QDs, the encapsulation ratio of QDs to CPV-VLPs under different concentrations was explored (Fig 4). As shown in Fig 4a and 4b, the particle size and absorbance property changed with the ratio of VLPs to QDs, which showed that the particle size increased with the quantity of QDs. Similarly, the absorbance of CPV-VLPs-QDs deviated from the specific absorbance curve of QDs. Combined with the results in Fig 4a and 4b, especially the TEM pictures, the ratio of VLPs to QDs 500:100 (v/v) was shown to be optimal. Therefore, the ratio of VLPs to QDs 500:100 was selected as the optimal ratio for all subsequent experiments.


Quantum Dots Encapsulated with Canine Parvovirus-Like Particles Improving the Cellular Targeted Labeling.

Yan D, Wang B, Sun S, Feng X, Jin Y, Yao X, Cao S, Guo H - PLoS ONE (2015)

Optimization of QDs encapsulation using CPV-VLPs.(a) Particle size of CPV-VLPs-QDs under different CPV-VLPs/QDs ratios. (b)Absorbance of complex at different CPV-VLPs/QDs ratios.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4580430&req=5

pone.0138883.g004: Optimization of QDs encapsulation using CPV-VLPs.(a) Particle size of CPV-VLPs-QDs under different CPV-VLPs/QDs ratios. (b)Absorbance of complex at different CPV-VLPs/QDs ratios.
Mentions: To ensure that VLPs encapsulated the QDs, the encapsulation ratio of QDs to CPV-VLPs under different concentrations was explored (Fig 4). As shown in Fig 4a and 4b, the particle size and absorbance property changed with the ratio of VLPs to QDs, which showed that the particle size increased with the quantity of QDs. Similarly, the absorbance of CPV-VLPs-QDs deviated from the specific absorbance curve of QDs. Combined with the results in Fig 4a and 4b, especially the TEM pictures, the ratio of VLPs to QDs 500:100 (v/v) was shown to be optimal. Therefore, the ratio of VLPs to QDs 500:100 was selected as the optimal ratio for all subsequent experiments.

Bottom Line: When incubated with different cell lines, CPV-VLPs-QDs significantly reduced the cytotoxicity of QDs and selectively labeled the cells with high-level transferrin receptors.Therefore, CPV-VLPs can be used as carriers to facilitate the targeted delivery of encapsulated nanomaterials into cells via receptor-mediated pathways.This study confirmed that CPV-VLPs can significantly promote the biocompatibility of nanomaterials and could expand the application of CPV-VLPs in biological medicine.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Veterinary Etiological Biology and National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu 730046, China.

ABSTRACT
Quantum dots (QDs) have a promising prospect in live-cell imaging and sensing because of unique fluorescence features. QDs aroused significant interest in the bio-imaging field through integrating the fluorescence properties of QDs and the delivery function of biomaterial. The natural tropism of Canine Parvovirus (CPV) to the transferrin receptor can target specific cells to increase the targeting ability of QDs in cell imaging. CPV virus-like particles (VLPs) from the expression of the CPV-VP2 capsid protein in a prokaryotic expression system were examined to encapsulate the QDs and deliver to cells with an expressed transferrin receptor. CPV-VLPs were used to encapsulate QDs that were modified using 3-mercaptopropionic acid. Gel electrophoresis, fluorescence spectrum, particle size, and transmission electron microscopy verified the conformation of a complex, in which QDs were encapsulated in CPV-VLPs (CPV-VLPs-QDs). When incubated with different cell lines, CPV-VLPs-QDs significantly reduced the cytotoxicity of QDs and selectively labeled the cells with high-level transferrin receptors. Cell-targeted labeling was achieved by utilizing the specific binding between the CPV capsid protein VP2 of VLPs and cellular receptors. CPV-VLPs-QDs, which can mimic the native CPV infection, can recognize and attach to the transferrin receptors on cellular membrane. Therefore, CPV-VLPs can be used as carriers to facilitate the targeted delivery of encapsulated nanomaterials into cells via receptor-mediated pathways. This study confirmed that CPV-VLPs can significantly promote the biocompatibility of nanomaterials and could expand the application of CPV-VLPs in biological medicine.

No MeSH data available.


Related in: MedlinePlus