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Three-Dimensional Histology Volume Reconstruction of Axonal Tract Tracing Data: Exploring Topographical Organization in Subcortical Projections from Rat Barrel Cortex.

Zakiewicz IM, Majka P, Wójcik DK, Bjaalie JG, Leergaard TB - PLoS ONE (2015)

Bottom Line: We here reconstruct serial histological images from four whole brains (originally acquired for conventional microscopic analysis) into volumetric images that are spatially registered to a 3-D atlas template.Our results further show that clusters of S1 corticostriatal and corticothalamic projections are distributed within narrow, elongated or spherical subspaces extending across the entire striatum / thalamus.The reconstructed image volumes are shared via the Rodent Brain Workbench (www.rbwb.org).

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway; Department of Neurophysiology, Nencki Institute of Experimental Biology, Warsaw, Poland.

ABSTRACT
Topographical organization is a hallmark of the mammalian brain, and the spatial organization of axonal connections in different brain regions provides a structural framework accommodating specific patterns of neural activity. The presence, amount, and spatial distribution of axonal connections are typically studied in tract tracing experiments in which axons or neurons are labeled and examined in histological sections. Three-dimensional (3-D) reconstruction techniques are used to achieve more complete visualization and improved understanding of complex topographical relationships. 3-D reconstruction approaches based on manually or semi-automatically recorded spatial points representing axonal labeling have been successfully applied for investigation of smaller brain regions, but are not practically feasible for whole-brain analysis of multiple regions. We here reconstruct serial histological images from four whole brains (originally acquired for conventional microscopic analysis) into volumetric images that are spatially registered to a 3-D atlas template. The aims were firstly to evaluate the quality of the 3-D reconstructions and the usefulness of the approach, and secondly to investigate axonal projection patterns and topographical organization in rat corticostriatal and corticothalamic pathways. We demonstrate that even with the limitations of the original routine histological material, the 3-D reconstructed volumetric images allow efficient visualization of tracer injection sites and axonal labeling, facilitating detection of spatial distributions and across-case comparisons. Our results further show that clusters of S1 corticostriatal and corticothalamic projections are distributed within narrow, elongated or spherical subspaces extending across the entire striatum / thalamus. We conclude that histology volume reconstructions facilitate mapping of spatial distribution patterns and topographical organization. The reconstructed image volumes are shared via the Rodent Brain Workbench (www.rbwb.org).

No MeSH data available.


Related in: MedlinePlus

3-D surface models of corticostriatal and corticothalamic projections.3-D visualization of topographical organization in corticostriatal and corticothalamic pathways. (A-D) Stereo-image pairs showing color coded 3-D surface models of the delineated axonal clusters in the striatum (A,B) and thalamus (C,D), in slightly offset views from anterior (A), medial (B,D), and posterior (C), as indicated in the inset figures (right column). To perceive the 3-D images the viewer must cross the eye axis to let the image pairs merge. (A,B) Clusters representing corticostriatal axons are distributed within a narrow, laminar subspace, extending in an anteroposterior direction through the dorsolateral striatum. (C,D) Clusters representing corticothalamic axons are distributed within a narrow, spherical shell-like subspace in the thalamus. A, anterior; D, dorsal; M, medial; P, posterior.
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pone.0137571.g005: 3-D surface models of corticostriatal and corticothalamic projections.3-D visualization of topographical organization in corticostriatal and corticothalamic pathways. (A-D) Stereo-image pairs showing color coded 3-D surface models of the delineated axonal clusters in the striatum (A,B) and thalamus (C,D), in slightly offset views from anterior (A), medial (B,D), and posterior (C), as indicated in the inset figures (right column). To perceive the 3-D images the viewer must cross the eye axis to let the image pairs merge. (A,B) Clusters representing corticostriatal axons are distributed within a narrow, laminar subspace, extending in an anteroposterior direction through the dorsolateral striatum. (C,D) Clusters representing corticothalamic axons are distributed within a narrow, spherical shell-like subspace in the thalamus. A, anterior; D, dorsal; M, medial; P, posterior.

Mentions: We have prepared geometric surface models demonstrating the shape and size of the labeling in each experiment. Since all data were registered to the same atlas space, differences in distribution could be readily observed by dynamic viewing or use of stereo image pairs (Fig 5).


Three-Dimensional Histology Volume Reconstruction of Axonal Tract Tracing Data: Exploring Topographical Organization in Subcortical Projections from Rat Barrel Cortex.

Zakiewicz IM, Majka P, Wójcik DK, Bjaalie JG, Leergaard TB - PLoS ONE (2015)

3-D surface models of corticostriatal and corticothalamic projections.3-D visualization of topographical organization in corticostriatal and corticothalamic pathways. (A-D) Stereo-image pairs showing color coded 3-D surface models of the delineated axonal clusters in the striatum (A,B) and thalamus (C,D), in slightly offset views from anterior (A), medial (B,D), and posterior (C), as indicated in the inset figures (right column). To perceive the 3-D images the viewer must cross the eye axis to let the image pairs merge. (A,B) Clusters representing corticostriatal axons are distributed within a narrow, laminar subspace, extending in an anteroposterior direction through the dorsolateral striatum. (C,D) Clusters representing corticothalamic axons are distributed within a narrow, spherical shell-like subspace in the thalamus. A, anterior; D, dorsal; M, medial; P, posterior.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4580429&req=5

pone.0137571.g005: 3-D surface models of corticostriatal and corticothalamic projections.3-D visualization of topographical organization in corticostriatal and corticothalamic pathways. (A-D) Stereo-image pairs showing color coded 3-D surface models of the delineated axonal clusters in the striatum (A,B) and thalamus (C,D), in slightly offset views from anterior (A), medial (B,D), and posterior (C), as indicated in the inset figures (right column). To perceive the 3-D images the viewer must cross the eye axis to let the image pairs merge. (A,B) Clusters representing corticostriatal axons are distributed within a narrow, laminar subspace, extending in an anteroposterior direction through the dorsolateral striatum. (C,D) Clusters representing corticothalamic axons are distributed within a narrow, spherical shell-like subspace in the thalamus. A, anterior; D, dorsal; M, medial; P, posterior.
Mentions: We have prepared geometric surface models demonstrating the shape and size of the labeling in each experiment. Since all data were registered to the same atlas space, differences in distribution could be readily observed by dynamic viewing or use of stereo image pairs (Fig 5).

Bottom Line: We here reconstruct serial histological images from four whole brains (originally acquired for conventional microscopic analysis) into volumetric images that are spatially registered to a 3-D atlas template.Our results further show that clusters of S1 corticostriatal and corticothalamic projections are distributed within narrow, elongated or spherical subspaces extending across the entire striatum / thalamus.The reconstructed image volumes are shared via the Rodent Brain Workbench (www.rbwb.org).

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway; Department of Neurophysiology, Nencki Institute of Experimental Biology, Warsaw, Poland.

ABSTRACT
Topographical organization is a hallmark of the mammalian brain, and the spatial organization of axonal connections in different brain regions provides a structural framework accommodating specific patterns of neural activity. The presence, amount, and spatial distribution of axonal connections are typically studied in tract tracing experiments in which axons or neurons are labeled and examined in histological sections. Three-dimensional (3-D) reconstruction techniques are used to achieve more complete visualization and improved understanding of complex topographical relationships. 3-D reconstruction approaches based on manually or semi-automatically recorded spatial points representing axonal labeling have been successfully applied for investigation of smaller brain regions, but are not practically feasible for whole-brain analysis of multiple regions. We here reconstruct serial histological images from four whole brains (originally acquired for conventional microscopic analysis) into volumetric images that are spatially registered to a 3-D atlas template. The aims were firstly to evaluate the quality of the 3-D reconstructions and the usefulness of the approach, and secondly to investigate axonal projection patterns and topographical organization in rat corticostriatal and corticothalamic pathways. We demonstrate that even with the limitations of the original routine histological material, the 3-D reconstructed volumetric images allow efficient visualization of tracer injection sites and axonal labeling, facilitating detection of spatial distributions and across-case comparisons. Our results further show that clusters of S1 corticostriatal and corticothalamic projections are distributed within narrow, elongated or spherical subspaces extending across the entire striatum / thalamus. We conclude that histology volume reconstructions facilitate mapping of spatial distribution patterns and topographical organization. The reconstructed image volumes are shared via the Rodent Brain Workbench (www.rbwb.org).

No MeSH data available.


Related in: MedlinePlus