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Telocytes in the Spleen.

Chang Y, Li C, Gan L, Li H, Guo Z - PLoS ONE (2015)

Bottom Line: Most telocytes had three or two telopods (28.71% and 22.58% respectively).Immunostaining indicated that these cells were positive for vimentin, CD34, nanog and sca-1, but negative for c-kit.These data prove the existence of telocytes in the spleen, which may serve as the experimental base for exploring their roles in the spleen.

View Article: PubMed Central - PubMed

Affiliation: Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang 453003, P.R. China; Department of Human Anatomy and Embryology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P.R. China.

ABSTRACT
Telocytes, a novel type of interstitial cells with very long and thin prolongations, have been identified in many organs in mammals. At present, the ultrastructural, immunocytochemical and electrophysiological properties of telocytes in multiple organs have been understood. However, telocytes in spleen, especially their roles in spleen have not been reported. The aim of this study was to investigate the ultrastructure, distribution and immunophenotypes of splenic telocytes. Rat spleen was harvested for the ultrastructure analysis by transmission electron microscopy (TEM). The primary culture of telocytes was performed after combined enzymatic digestion. The characteristic morphology was analyzed by a scanning electron microscopy (SEM). It was shown that telocytes displayed a piriform/spindle/triangular shape with long and slender telopods and extremely long prolongation contracting with surrounding cells in the spleen. Their dynamic profiles of cytoplasmic separation were recorded by the Live Cell Imaging System. The length of telopods was mostly distributing in 20-30 μm, in accordance with normal distribution. Most telocytes had three or two telopods (28.71% and 22.58% respectively). Immunostaining indicated that these cells were positive for vimentin, CD34, nanog and sca-1, but negative for c-kit. These data prove the existence of telocytes in the spleen, which may serve as the experimental base for exploring their roles in the spleen.

No MeSH data available.


Related in: MedlinePlus

Double immunofluorescence staining for splenic TCs cultured in vitro A,E,I.Vimentin immunostaining (A. Cy3 fluorescence labeling, red; E,I. FITC fluorescence labeling, green); B,F,J. CD34, nanog and sca-1 immunostaining respectively (B. FITC fluorescence labeling, green; F,J. Cy3 fluorescence labeling, red); C,G,K. nuclei of TCs (DAPI staining, blue), D. merged image of A, B and C; H. merged image of E, F and G; L. merged image of I, J and K. Scale bar = 20μm.
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pone.0138851.g007: Double immunofluorescence staining for splenic TCs cultured in vitro A,E,I.Vimentin immunostaining (A. Cy3 fluorescence labeling, red; E,I. FITC fluorescence labeling, green); B,F,J. CD34, nanog and sca-1 immunostaining respectively (B. FITC fluorescence labeling, green; F,J. Cy3 fluorescence labeling, red); C,G,K. nuclei of TCs (DAPI staining, blue), D. merged image of A, B and C; H. merged image of E, F and G; L. merged image of I, J and K. Scale bar = 20μm.

Mentions: TCs were positive for vimentin, the cytoskeletal component responsible for maintaining cell integritystabilizing cytoskeletal interactions, located in cell body and their prolongations (Fig 6). TCs also expressed CD34, nanog and sca-1. As shown in Fig 6, it appeared strongly positive for nanog and sca-1 in cell body and the initiation part of the prolongations. However, c-kit was negative in splentic TCs(Data not shown). Then double immunofluorescence staining to detect the TCs demonstrated there were co-expression of vimentin and CD34, vimentin and nanog, vimentin and sca-1 respectively (Fig 7). These phenotypes were stable following sub-culturing.


Telocytes in the Spleen.

Chang Y, Li C, Gan L, Li H, Guo Z - PLoS ONE (2015)

Double immunofluorescence staining for splenic TCs cultured in vitro A,E,I.Vimentin immunostaining (A. Cy3 fluorescence labeling, red; E,I. FITC fluorescence labeling, green); B,F,J. CD34, nanog and sca-1 immunostaining respectively (B. FITC fluorescence labeling, green; F,J. Cy3 fluorescence labeling, red); C,G,K. nuclei of TCs (DAPI staining, blue), D. merged image of A, B and C; H. merged image of E, F and G; L. merged image of I, J and K. Scale bar = 20μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4580423&req=5

pone.0138851.g007: Double immunofluorescence staining for splenic TCs cultured in vitro A,E,I.Vimentin immunostaining (A. Cy3 fluorescence labeling, red; E,I. FITC fluorescence labeling, green); B,F,J. CD34, nanog and sca-1 immunostaining respectively (B. FITC fluorescence labeling, green; F,J. Cy3 fluorescence labeling, red); C,G,K. nuclei of TCs (DAPI staining, blue), D. merged image of A, B and C; H. merged image of E, F and G; L. merged image of I, J and K. Scale bar = 20μm.
Mentions: TCs were positive for vimentin, the cytoskeletal component responsible for maintaining cell integritystabilizing cytoskeletal interactions, located in cell body and their prolongations (Fig 6). TCs also expressed CD34, nanog and sca-1. As shown in Fig 6, it appeared strongly positive for nanog and sca-1 in cell body and the initiation part of the prolongations. However, c-kit was negative in splentic TCs(Data not shown). Then double immunofluorescence staining to detect the TCs demonstrated there were co-expression of vimentin and CD34, vimentin and nanog, vimentin and sca-1 respectively (Fig 7). These phenotypes were stable following sub-culturing.

Bottom Line: Most telocytes had three or two telopods (28.71% and 22.58% respectively).Immunostaining indicated that these cells were positive for vimentin, CD34, nanog and sca-1, but negative for c-kit.These data prove the existence of telocytes in the spleen, which may serve as the experimental base for exploring their roles in the spleen.

View Article: PubMed Central - PubMed

Affiliation: Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang 453003, P.R. China; Department of Human Anatomy and Embryology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P.R. China.

ABSTRACT
Telocytes, a novel type of interstitial cells with very long and thin prolongations, have been identified in many organs in mammals. At present, the ultrastructural, immunocytochemical and electrophysiological properties of telocytes in multiple organs have been understood. However, telocytes in spleen, especially their roles in spleen have not been reported. The aim of this study was to investigate the ultrastructure, distribution and immunophenotypes of splenic telocytes. Rat spleen was harvested for the ultrastructure analysis by transmission electron microscopy (TEM). The primary culture of telocytes was performed after combined enzymatic digestion. The characteristic morphology was analyzed by a scanning electron microscopy (SEM). It was shown that telocytes displayed a piriform/spindle/triangular shape with long and slender telopods and extremely long prolongation contracting with surrounding cells in the spleen. Their dynamic profiles of cytoplasmic separation were recorded by the Live Cell Imaging System. The length of telopods was mostly distributing in 20-30 μm, in accordance with normal distribution. Most telocytes had three or two telopods (28.71% and 22.58% respectively). Immunostaining indicated that these cells were positive for vimentin, CD34, nanog and sca-1, but negative for c-kit. These data prove the existence of telocytes in the spleen, which may serve as the experimental base for exploring their roles in the spleen.

No MeSH data available.


Related in: MedlinePlus