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Isolation of a Novel Phage with Activity against Streptococcus mutans Biofilms.

Dalmasso M, de Haas E, Neve H, Strain R, Cousin FJ, Stockdale SR, Ross RP, Hill C - PLoS ONE (2015)

Bottom Line: Its genome was closely related to the only two other available S. mutans phage genomes, M102 and M102AD. ɸAPCM01 inhibited the growth of S. mutans strain DPC6143 within hours in broth and in artificial saliva at multiplicity of infections as low as 2.5x10-5.In the presence of phage ɸAPCM01 the metabolic activity of a S. mutans biofilm was reduced after 24 h of contact and did not increased again after 48 h, and the live cells in the biofilm decreased by at least 5 log cfu/ml.Despite its narrow host range, this newly isolated S. mutans phage exhibits promising antimicrobial properties.

View Article: PubMed Central - PubMed

Affiliation: School of Microbiology, University College Cork, Cork, Ireland; APC Microbiome Institute, University College Cork, Cork, Ireland.

ABSTRACT
Streptococcus mutans is one of the principal agents of caries formation mainly, because of its ability to form biofilms at the tooth surface. Bacteriophages (phages) are promising antimicrobial agents that could be used to prevent or treat caries formation by S. mutans. The aim of this study was to isolate new S. mutans phages and to characterize their antimicrobial properties. A new phage, ɸAPCM01, was isolated from a human saliva sample. Its genome was closely related to the only two other available S. mutans phage genomes, M102 and M102AD. ɸAPCM01 inhibited the growth of S. mutans strain DPC6143 within hours in broth and in artificial saliva at multiplicity of infections as low as 2.5x10-5. In the presence of phage ɸAPCM01 the metabolic activity of a S. mutans biofilm was reduced after 24 h of contact and did not increased again after 48 h, and the live cells in the biofilm decreased by at least 5 log cfu/ml. Despite its narrow host range, this newly isolated S. mutans phage exhibits promising antimicrobial properties.

No MeSH data available.


Related in: MedlinePlus

One-step growth curve of phage ɸAPCM01 with S. mutans strain DPC6143 in BHI broth at 37°C.Three independent experiments were carried out. Error-bars indicate standard deviation.
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pone.0138651.g002: One-step growth curve of phage ɸAPCM01 with S. mutans strain DPC6143 in BHI broth at 37°C.Three independent experiments were carried out. Error-bars indicate standard deviation.

Mentions: A one-step growth curve was performed to assess the population dynamics of ɸAPCM01 in the presence of S. mutans strain DPC6143. ɸAPCM01 had a latent period of 60 min and a rise phase of 60 min before reaching the plateau phase (Fig 2). The burst size was calculated as 44.2 ± 9.8 phage particles.


Isolation of a Novel Phage with Activity against Streptococcus mutans Biofilms.

Dalmasso M, de Haas E, Neve H, Strain R, Cousin FJ, Stockdale SR, Ross RP, Hill C - PLoS ONE (2015)

One-step growth curve of phage ɸAPCM01 with S. mutans strain DPC6143 in BHI broth at 37°C.Three independent experiments were carried out. Error-bars indicate standard deviation.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4580409&req=5

pone.0138651.g002: One-step growth curve of phage ɸAPCM01 with S. mutans strain DPC6143 in BHI broth at 37°C.Three independent experiments were carried out. Error-bars indicate standard deviation.
Mentions: A one-step growth curve was performed to assess the population dynamics of ɸAPCM01 in the presence of S. mutans strain DPC6143. ɸAPCM01 had a latent period of 60 min and a rise phase of 60 min before reaching the plateau phase (Fig 2). The burst size was calculated as 44.2 ± 9.8 phage particles.

Bottom Line: Its genome was closely related to the only two other available S. mutans phage genomes, M102 and M102AD. ɸAPCM01 inhibited the growth of S. mutans strain DPC6143 within hours in broth and in artificial saliva at multiplicity of infections as low as 2.5x10-5.In the presence of phage ɸAPCM01 the metabolic activity of a S. mutans biofilm was reduced after 24 h of contact and did not increased again after 48 h, and the live cells in the biofilm decreased by at least 5 log cfu/ml.Despite its narrow host range, this newly isolated S. mutans phage exhibits promising antimicrobial properties.

View Article: PubMed Central - PubMed

Affiliation: School of Microbiology, University College Cork, Cork, Ireland; APC Microbiome Institute, University College Cork, Cork, Ireland.

ABSTRACT
Streptococcus mutans is one of the principal agents of caries formation mainly, because of its ability to form biofilms at the tooth surface. Bacteriophages (phages) are promising antimicrobial agents that could be used to prevent or treat caries formation by S. mutans. The aim of this study was to isolate new S. mutans phages and to characterize their antimicrobial properties. A new phage, ɸAPCM01, was isolated from a human saliva sample. Its genome was closely related to the only two other available S. mutans phage genomes, M102 and M102AD. ɸAPCM01 inhibited the growth of S. mutans strain DPC6143 within hours in broth and in artificial saliva at multiplicity of infections as low as 2.5x10-5. In the presence of phage ɸAPCM01 the metabolic activity of a S. mutans biofilm was reduced after 24 h of contact and did not increased again after 48 h, and the live cells in the biofilm decreased by at least 5 log cfu/ml. Despite its narrow host range, this newly isolated S. mutans phage exhibits promising antimicrobial properties.

No MeSH data available.


Related in: MedlinePlus