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Association of the FCN2 Gene Single Nucleotide Polymorphisms with Susceptibility to Pulmonary Tuberculosis.

Xu DD, Wang C, Jiang F, Wei LL, Shi LY, Yu XM, Liu CM, Liu XH, Feng XM, Ping ZP, Jiang TT, Chen ZL, Li ZJ, Li JC - PLoS ONE (2015)

Bottom Line: Ficolin-2 (FCN2) is an innate immune pattern recognition molecule that can activate the complement pathway, opsonophagocytosis, and elimination of the pathogens.There was strong linkage disequilibrium (r2 > 0.80, P < 0.0001) between 7 SNPs except the -602 G>A site.This study provides a novel idea for the prevention and control of TB transmission from a genetics perspective.

View Article: PubMed Central - PubMed

Affiliation: Institute of Cell Biology, Zhejiang University, Hangzhou 310058, P.R. China.

ABSTRACT
Ficolin-2 (FCN2) is an innate immune pattern recognition molecule that can activate the complement pathway, opsonophagocytosis, and elimination of the pathogens. The present study aimed to investigate the association of the FCN2 gene single nucleotide polymorphisms (SNPs) with susceptibility to pulmonary tuberculosis (TB). A total of seven SNPs in exon 8 (+6359 C>T and +6424 G>T) and in the promoter region (-986 G>A, -602 G>A, -557 A>G, -64 A>C and -4 A>G) of the FCN2 gene were genotyped using the PCR amplification and DNA sequencing methods in the healthy controls group (n = 254) and the pulmonary TB group (n = 282). The correlation between SNPs and pulmonary TB was analyzed using the logistic regression method. The results showed that there were no significant differences in the distribution of allelic frequencies of seven SNPs between the pulmonary TB group and the healthy controls group. However, the frequency of the variant homozygous genotype (P = 0.037, -557 A>G; P = 0.038, -64 A>C; P = 0.024, +6424 G>T) in the TB group was significantly lower than the control group. After adjustment for age and gender, these variant homozygous genotypes were found to be recessive models in association with pulmonary TB. In addition, -64 A>C (P = 0.047) and +6424 G>T (P = 0.03) were found to be codominant models in association with pulmonary TB. There was strong linkage disequilibrium (r2 > 0.80, P < 0.0001) between 7 SNPs except the -602 G>A site. Therefore, -557 A>G, -64 A>C and +6424 G>T SNPs of the FCN2 gene were correlated with pulmonary TB, and may be protective factors for TB. This study provides a novel idea for the prevention and control of TB transmission from a genetics perspective.

No MeSH data available.


Related in: MedlinePlus

The DNA sequences with 7 SNPs in the FCN2 gene.
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pone.0138356.g001: The DNA sequences with 7 SNPs in the FCN2 gene.

Mentions: Seven SNPs (-986 G>A, -602 G>A, -557 A>G, -64 A>C, -4 A>G, +6359 C>T, and +6424 G>T) in the FCN2 gene were sequenced after PCR amplification (Fig 1). The allelic distributions of FCN2 SNPs were in accordance with Hardy-Weinberg equilibrium in the control group (P > 0.05) (Table 1). There were no significant differences in allele frequencies between the pulmonary TB group and the healthy controls group (P > 0.05) (Table 1).


Association of the FCN2 Gene Single Nucleotide Polymorphisms with Susceptibility to Pulmonary Tuberculosis.

Xu DD, Wang C, Jiang F, Wei LL, Shi LY, Yu XM, Liu CM, Liu XH, Feng XM, Ping ZP, Jiang TT, Chen ZL, Li ZJ, Li JC - PLoS ONE (2015)

The DNA sequences with 7 SNPs in the FCN2 gene.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4574923&req=5

pone.0138356.g001: The DNA sequences with 7 SNPs in the FCN2 gene.
Mentions: Seven SNPs (-986 G>A, -602 G>A, -557 A>G, -64 A>C, -4 A>G, +6359 C>T, and +6424 G>T) in the FCN2 gene were sequenced after PCR amplification (Fig 1). The allelic distributions of FCN2 SNPs were in accordance with Hardy-Weinberg equilibrium in the control group (P > 0.05) (Table 1). There were no significant differences in allele frequencies between the pulmonary TB group and the healthy controls group (P > 0.05) (Table 1).

Bottom Line: Ficolin-2 (FCN2) is an innate immune pattern recognition molecule that can activate the complement pathway, opsonophagocytosis, and elimination of the pathogens.There was strong linkage disequilibrium (r2 > 0.80, P < 0.0001) between 7 SNPs except the -602 G>A site.This study provides a novel idea for the prevention and control of TB transmission from a genetics perspective.

View Article: PubMed Central - PubMed

Affiliation: Institute of Cell Biology, Zhejiang University, Hangzhou 310058, P.R. China.

ABSTRACT
Ficolin-2 (FCN2) is an innate immune pattern recognition molecule that can activate the complement pathway, opsonophagocytosis, and elimination of the pathogens. The present study aimed to investigate the association of the FCN2 gene single nucleotide polymorphisms (SNPs) with susceptibility to pulmonary tuberculosis (TB). A total of seven SNPs in exon 8 (+6359 C>T and +6424 G>T) and in the promoter region (-986 G>A, -602 G>A, -557 A>G, -64 A>C and -4 A>G) of the FCN2 gene were genotyped using the PCR amplification and DNA sequencing methods in the healthy controls group (n = 254) and the pulmonary TB group (n = 282). The correlation between SNPs and pulmonary TB was analyzed using the logistic regression method. The results showed that there were no significant differences in the distribution of allelic frequencies of seven SNPs between the pulmonary TB group and the healthy controls group. However, the frequency of the variant homozygous genotype (P = 0.037, -557 A>G; P = 0.038, -64 A>C; P = 0.024, +6424 G>T) in the TB group was significantly lower than the control group. After adjustment for age and gender, these variant homozygous genotypes were found to be recessive models in association with pulmonary TB. In addition, -64 A>C (P = 0.047) and +6424 G>T (P = 0.03) were found to be codominant models in association with pulmonary TB. There was strong linkage disequilibrium (r2 > 0.80, P < 0.0001) between 7 SNPs except the -602 G>A site. Therefore, -557 A>G, -64 A>C and +6424 G>T SNPs of the FCN2 gene were correlated with pulmonary TB, and may be protective factors for TB. This study provides a novel idea for the prevention and control of TB transmission from a genetics perspective.

No MeSH data available.


Related in: MedlinePlus