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Osteogenic Surface Modification Based on Functionalized Poly-P-Xylylene Coating.

Chang CH, Yeh SY, Lee BH, Chen CJ, Su CT, Lin YT, Liu CL, Chen HY - PLoS ONE (2015)

Bottom Line: The biotechnology to immobilize biomolecules on material surfaces has been developed vigorously due to its high potentials in medical applications.This modified surface induces differentiation of preosteoblast into osteoblast, manifested by alkaline phosphatase (ALP) activity assay, Alizarin Red S (ARS) staining and the expression of osteogenic gene markers, Alpl and Bglap3.With this coating technology, immobilization of growth factors onto material surface can be achieved more simply and more effectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.

ABSTRACT
The biotechnology to immobilize biomolecules on material surfaces has been developed vigorously due to its high potentials in medical applications. In this study, a simple and effective method was designed to immobilize biomolecules via amine-N-hydroxysuccinimide (NHS) ester conjugation reaction using functionalized poly-p-xylylene coating on material surfaces. The NHS ester functionalized coating is synthesized via chemical vapor deposition, a facile and solvent-less method, creating a surface which is ready to perform a one-step conjugation reaction. Bone morphogenetic protein 2 (BMP-2) is immobilized onto material surfaces by this coating method, forming an osteogenic environment. The immobilization process is controlled at a low temperature which does not damage proteins. This modified surface induces differentiation of preosteoblast into osteoblast, manifested by alkaline phosphatase (ALP) activity assay, Alizarin Red S (ARS) staining and the expression of osteogenic gene markers, Alpl and Bglap3. With this coating technology, immobilization of growth factors onto material surface can be achieved more simply and more effectively.

No MeSH data available.


Related in: MedlinePlus

XPS survey spectra of (a) coating 5 modified surface and (b) coating 5/BMP-2 modified surface.The atomic concentration of C, N and O are 77.3%, 4.55% and 18.22% on the coating 5 modified surface and 65.0%, 9.5% and 25.5% on the coating 5/BMP-2 modified surface. The N composition significantly increased due to immobilization of BMP-2.
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pone.0137017.g003: XPS survey spectra of (a) coating 5 modified surface and (b) coating 5/BMP-2 modified surface.The atomic concentration of C, N and O are 77.3%, 4.55% and 18.22% on the coating 5 modified surface and 65.0%, 9.5% and 25.5% on the coating 5/BMP-2 modified surface. The N composition significantly increased due to immobilization of BMP-2.

Mentions: Characterizations using a combination of IRRAS and XPS have confirmed the characteristic band vibrations and chemical compositions of coating 5. As shown in Fig 2(A), characteristic peak at 1716 cm-1 1739 cm-1 and 1770 cm-1 were the carbonyl stretches from NHS ester groups. The IRRAS results after immobilization of BMP-2 are shown in Fig 2(B). The ratio between 1709 cm-1 and 1741 cm-1 carbonyl stretches declined and N-H and O-H characteristic band around 3247 cm-1 and 3490 cm-1 were detected due to the proteins immobilized on surface. The XPS data further confirmed the immobilization of BMP-2. As shown in Fig 3, the atomic concentration of the coating 5 modified surface is 77.3 atom% for carbon, 4.5 atom% for nitrogen and 18.2 atom% for oxygen. After immobilization of BMP-2 the atomic concentration became 65.0 atom% for carbon, 9.5 atom% for nitrogen and 25.5 atom% for oxygen. The concentration of nitrogen and oxygen both increase because of the immobilization of BMP-2 on the surface.


Osteogenic Surface Modification Based on Functionalized Poly-P-Xylylene Coating.

Chang CH, Yeh SY, Lee BH, Chen CJ, Su CT, Lin YT, Liu CL, Chen HY - PLoS ONE (2015)

XPS survey spectra of (a) coating 5 modified surface and (b) coating 5/BMP-2 modified surface.The atomic concentration of C, N and O are 77.3%, 4.55% and 18.22% on the coating 5 modified surface and 65.0%, 9.5% and 25.5% on the coating 5/BMP-2 modified surface. The N composition significantly increased due to immobilization of BMP-2.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4574780&req=5

pone.0137017.g003: XPS survey spectra of (a) coating 5 modified surface and (b) coating 5/BMP-2 modified surface.The atomic concentration of C, N and O are 77.3%, 4.55% and 18.22% on the coating 5 modified surface and 65.0%, 9.5% and 25.5% on the coating 5/BMP-2 modified surface. The N composition significantly increased due to immobilization of BMP-2.
Mentions: Characterizations using a combination of IRRAS and XPS have confirmed the characteristic band vibrations and chemical compositions of coating 5. As shown in Fig 2(A), characteristic peak at 1716 cm-1 1739 cm-1 and 1770 cm-1 were the carbonyl stretches from NHS ester groups. The IRRAS results after immobilization of BMP-2 are shown in Fig 2(B). The ratio between 1709 cm-1 and 1741 cm-1 carbonyl stretches declined and N-H and O-H characteristic band around 3247 cm-1 and 3490 cm-1 were detected due to the proteins immobilized on surface. The XPS data further confirmed the immobilization of BMP-2. As shown in Fig 3, the atomic concentration of the coating 5 modified surface is 77.3 atom% for carbon, 4.5 atom% for nitrogen and 18.2 atom% for oxygen. After immobilization of BMP-2 the atomic concentration became 65.0 atom% for carbon, 9.5 atom% for nitrogen and 25.5 atom% for oxygen. The concentration of nitrogen and oxygen both increase because of the immobilization of BMP-2 on the surface.

Bottom Line: The biotechnology to immobilize biomolecules on material surfaces has been developed vigorously due to its high potentials in medical applications.This modified surface induces differentiation of preosteoblast into osteoblast, manifested by alkaline phosphatase (ALP) activity assay, Alizarin Red S (ARS) staining and the expression of osteogenic gene markers, Alpl and Bglap3.With this coating technology, immobilization of growth factors onto material surface can be achieved more simply and more effectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.

ABSTRACT
The biotechnology to immobilize biomolecules on material surfaces has been developed vigorously due to its high potentials in medical applications. In this study, a simple and effective method was designed to immobilize biomolecules via amine-N-hydroxysuccinimide (NHS) ester conjugation reaction using functionalized poly-p-xylylene coating on material surfaces. The NHS ester functionalized coating is synthesized via chemical vapor deposition, a facile and solvent-less method, creating a surface which is ready to perform a one-step conjugation reaction. Bone morphogenetic protein 2 (BMP-2) is immobilized onto material surfaces by this coating method, forming an osteogenic environment. The immobilization process is controlled at a low temperature which does not damage proteins. This modified surface induces differentiation of preosteoblast into osteoblast, manifested by alkaline phosphatase (ALP) activity assay, Alizarin Red S (ARS) staining and the expression of osteogenic gene markers, Alpl and Bglap3. With this coating technology, immobilization of growth factors onto material surface can be achieved more simply and more effectively.

No MeSH data available.


Related in: MedlinePlus