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The Antibacterial Activity of Acetic Acid against Biofilm-Producing Pathogens of Relevance to Burns Patients.

Halstead FD, Rauf M, Moiemen NS, Bamford A, Wearn CM, Fraise AP, Lund PA, Oppenheim BA, Webber MA - PLoS ONE (2015)

Bottom Line: Twenty-nine isolates of common wound-infecting pathogens were tested.Acetic acid was antibacterial against planktonic growth, with an minimum inhibitory concentration of 0.16-0.31% for all isolates, and was also able to prevent formation of biofilms (at 0.31%).Eradication of mature biofilms was observed for all isolates after three hours of exposure.

View Article: PubMed Central - PubMed

Affiliation: Queen Elizabeth Hospital, University Hospitals Birmingham NHS Foundation Trust, Birmingham, United Kingdom; NIHR Surgical Reconstruction and Microbiology Research Centre, Queen Elizabeth Hospital, Birmingham, United Kingdom; Institute of Microbiology and Infection, School of Biosciences, College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom.

ABSTRACT

Introduction: Localised infections, and burn wound sepsis are key concerns in the treatment of burns patients, and prevention of colonisation largely relies on biocides. Acetic acid has been shown to have good antibacterial activity against various planktonic organisms, however data is limited on efficacy, and few studies have been performed on biofilms.

Objectives: We sought to investigate the antibacterial activity of acetic acid against important burn wound colonising organisms growing planktonically and as biofilms.

Methods: Laboratory experiments were performed to test the ability of acetic acid to inhibit growth of pathogens, inhibit the formation of biofilms, and eradicate pre-formed biofilms.

Results: Twenty-nine isolates of common wound-infecting pathogens were tested. Acetic acid was antibacterial against planktonic growth, with an minimum inhibitory concentration of 0.16-0.31% for all isolates, and was also able to prevent formation of biofilms (at 0.31%). Eradication of mature biofilms was observed for all isolates after three hours of exposure.

Conclusions: This study provides evidence that acetic acid can inhibit growth of key burn wound pathogens when used at very dilute concentrations. Owing to current concerns of the reducing efficacy of systemic antibiotics, this novel biocide application offers great promise as a cheap and effective measure to treat infections in burns patients.

No MeSH data available.


Related in: MedlinePlus

Graph showing the MBEC results for isolates AB_AYE and MDR_A.Optical density on the y axis refers to the average biofilm seeding for isolates AB_AYE and MDR_A after 3 hours of exposure to AA at the range of dilutions tested. POS: positive control, NEG: negative (broth only) control. The vertical line represents the MBEC, the error bars represent the standard error, and asterisks denote dilutions with statistically significant reductions in the seeding of the biofilm according to the t-test.
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pone.0136190.g003: Graph showing the MBEC results for isolates AB_AYE and MDR_A.Optical density on the y axis refers to the average biofilm seeding for isolates AB_AYE and MDR_A after 3 hours of exposure to AA at the range of dilutions tested. POS: positive control, NEG: negative (broth only) control. The vertical line represents the MBEC, the error bars represent the standard error, and asterisks denote dilutions with statistically significant reductions in the seeding of the biofilm according to the t-test.

Mentions: For the 22 isolates tested, incubation of the biofilm-coated peg plate for three hours at 33°C in the range of dilutions of AA (5–0.01%) resulted in a statistically significant reduction in seeding. This was visualised by plotting a graph of the detector broth OD (with high numbers indicating turbidity and hence seeding of a viable biofilm), and then measured by subjecting all AA dilutions and the positive control (the overnight bacterial cultures in the absence of AA) to the paired t-test. The lowest concentration of AA where there was minimal seeding of the biofilm and a p value of ≤0.05% was recorded as the MBEC (Table 2). Fig 3 shows randomly selected representative MBEC data for AB_AYE and MDR_A. Graphs showing the MBEC for all the isolates can be found in the supplementary results (S3 Fig).


The Antibacterial Activity of Acetic Acid against Biofilm-Producing Pathogens of Relevance to Burns Patients.

Halstead FD, Rauf M, Moiemen NS, Bamford A, Wearn CM, Fraise AP, Lund PA, Oppenheim BA, Webber MA - PLoS ONE (2015)

Graph showing the MBEC results for isolates AB_AYE and MDR_A.Optical density on the y axis refers to the average biofilm seeding for isolates AB_AYE and MDR_A after 3 hours of exposure to AA at the range of dilutions tested. POS: positive control, NEG: negative (broth only) control. The vertical line represents the MBEC, the error bars represent the standard error, and asterisks denote dilutions with statistically significant reductions in the seeding of the biofilm according to the t-test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4566994&req=5

pone.0136190.g003: Graph showing the MBEC results for isolates AB_AYE and MDR_A.Optical density on the y axis refers to the average biofilm seeding for isolates AB_AYE and MDR_A after 3 hours of exposure to AA at the range of dilutions tested. POS: positive control, NEG: negative (broth only) control. The vertical line represents the MBEC, the error bars represent the standard error, and asterisks denote dilutions with statistically significant reductions in the seeding of the biofilm according to the t-test.
Mentions: For the 22 isolates tested, incubation of the biofilm-coated peg plate for three hours at 33°C in the range of dilutions of AA (5–0.01%) resulted in a statistically significant reduction in seeding. This was visualised by plotting a graph of the detector broth OD (with high numbers indicating turbidity and hence seeding of a viable biofilm), and then measured by subjecting all AA dilutions and the positive control (the overnight bacterial cultures in the absence of AA) to the paired t-test. The lowest concentration of AA where there was minimal seeding of the biofilm and a p value of ≤0.05% was recorded as the MBEC (Table 2). Fig 3 shows randomly selected representative MBEC data for AB_AYE and MDR_A. Graphs showing the MBEC for all the isolates can be found in the supplementary results (S3 Fig).

Bottom Line: Twenty-nine isolates of common wound-infecting pathogens were tested.Acetic acid was antibacterial against planktonic growth, with an minimum inhibitory concentration of 0.16-0.31% for all isolates, and was also able to prevent formation of biofilms (at 0.31%).Eradication of mature biofilms was observed for all isolates after three hours of exposure.

View Article: PubMed Central - PubMed

Affiliation: Queen Elizabeth Hospital, University Hospitals Birmingham NHS Foundation Trust, Birmingham, United Kingdom; NIHR Surgical Reconstruction and Microbiology Research Centre, Queen Elizabeth Hospital, Birmingham, United Kingdom; Institute of Microbiology and Infection, School of Biosciences, College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom.

ABSTRACT

Introduction: Localised infections, and burn wound sepsis are key concerns in the treatment of burns patients, and prevention of colonisation largely relies on biocides. Acetic acid has been shown to have good antibacterial activity against various planktonic organisms, however data is limited on efficacy, and few studies have been performed on biofilms.

Objectives: We sought to investigate the antibacterial activity of acetic acid against important burn wound colonising organisms growing planktonically and as biofilms.

Methods: Laboratory experiments were performed to test the ability of acetic acid to inhibit growth of pathogens, inhibit the formation of biofilms, and eradicate pre-formed biofilms.

Results: Twenty-nine isolates of common wound-infecting pathogens were tested. Acetic acid was antibacterial against planktonic growth, with an minimum inhibitory concentration of 0.16-0.31% for all isolates, and was also able to prevent formation of biofilms (at 0.31%). Eradication of mature biofilms was observed for all isolates after three hours of exposure.

Conclusions: This study provides evidence that acetic acid can inhibit growth of key burn wound pathogens when used at very dilute concentrations. Owing to current concerns of the reducing efficacy of systemic antibiotics, this novel biocide application offers great promise as a cheap and effective measure to treat infections in burns patients.

No MeSH data available.


Related in: MedlinePlus