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Salt-induced transcription factor MYB74 is regulated by the RNA-directed DNA methylation pathway in Arabidopsis.

Xu R, Wang Y, Zheng H, Lu W, Wu C, Huang J, Yan K, Yang G, Zheng C - J. Exp. Bot. (2015)

Bottom Line: Levels of DNA methylation in this region were significantly reduced in wild type plants under salt stress, whereas no changes were found in RdDM mutants.Northern blot and quantitative real-time reverse transcription PCR analysis showed that the accumulation of 24-nt siRNAs was decreased in WT plants under salt stress.These results suggest that changes in the levels of the five 24-nt siRNAs regulate the AtMYB74 transcription factor via RdDM in response to salt stress.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, PR China.

No MeSH data available.


Related in: MedlinePlus

Schematic model of the transcriptional regulation of salt stress signalling via MYB, showing the involvement of a putative R2R3-MYB transcription factor member, AtMYB74, regulated by RdDM, in controlling the positive regulation of transcriptional responses to salt stress. The purple arrow represents results obtained from previous studies, and the green arrow represents the results of the present study. (This figure is available in colour at JXB online.)
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Figure 6: Schematic model of the transcriptional regulation of salt stress signalling via MYB, showing the involvement of a putative R2R3-MYB transcription factor member, AtMYB74, regulated by RdDM, in controlling the positive regulation of transcriptional responses to salt stress. The purple arrow represents results obtained from previous studies, and the green arrow represents the results of the present study. (This figure is available in colour at JXB online.)

Mentions: The salt stress signal transduction pathway is far more complex than has been suggested previously. As shown in Fig 6, previous studies have shown that some R2R3-MYB transcription factors, such as AtMYB2, AtMYB20, and AtMYB73, participate in the regulation of gene expression under salt stress (Abe et al., 2003; Cui et al., 2013; Kim et al., 2013). The present study demonstrated the involvement of AtMYB74, a R2R3-MYB family member, which is regulated by RdDM in controlling the positive regulation of transcriptional responses to salt stress. Salt stress induces the RdDM pathway via repressing the accumulation of siRNAs to activate AtMYB74 expression, which then transduces the related signalling into downstream processes that endow resistance to such stress.


Salt-induced transcription factor MYB74 is regulated by the RNA-directed DNA methylation pathway in Arabidopsis.

Xu R, Wang Y, Zheng H, Lu W, Wu C, Huang J, Yan K, Yang G, Zheng C - J. Exp. Bot. (2015)

Schematic model of the transcriptional regulation of salt stress signalling via MYB, showing the involvement of a putative R2R3-MYB transcription factor member, AtMYB74, regulated by RdDM, in controlling the positive regulation of transcriptional responses to salt stress. The purple arrow represents results obtained from previous studies, and the green arrow represents the results of the present study. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4566987&req=5

Figure 6: Schematic model of the transcriptional regulation of salt stress signalling via MYB, showing the involvement of a putative R2R3-MYB transcription factor member, AtMYB74, regulated by RdDM, in controlling the positive regulation of transcriptional responses to salt stress. The purple arrow represents results obtained from previous studies, and the green arrow represents the results of the present study. (This figure is available in colour at JXB online.)
Mentions: The salt stress signal transduction pathway is far more complex than has been suggested previously. As shown in Fig 6, previous studies have shown that some R2R3-MYB transcription factors, such as AtMYB2, AtMYB20, and AtMYB73, participate in the regulation of gene expression under salt stress (Abe et al., 2003; Cui et al., 2013; Kim et al., 2013). The present study demonstrated the involvement of AtMYB74, a R2R3-MYB family member, which is regulated by RdDM in controlling the positive regulation of transcriptional responses to salt stress. Salt stress induces the RdDM pathway via repressing the accumulation of siRNAs to activate AtMYB74 expression, which then transduces the related signalling into downstream processes that endow resistance to such stress.

Bottom Line: Levels of DNA methylation in this region were significantly reduced in wild type plants under salt stress, whereas no changes were found in RdDM mutants.Northern blot and quantitative real-time reverse transcription PCR analysis showed that the accumulation of 24-nt siRNAs was decreased in WT plants under salt stress.These results suggest that changes in the levels of the five 24-nt siRNAs regulate the AtMYB74 transcription factor via RdDM in response to salt stress.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, PR China.

No MeSH data available.


Related in: MedlinePlus