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Salt-induced transcription factor MYB74 is regulated by the RNA-directed DNA methylation pathway in Arabidopsis.

Xu R, Wang Y, Zheng H, Lu W, Wu C, Huang J, Yan K, Yang G, Zheng C - J. Exp. Bot. (2015)

Bottom Line: Levels of DNA methylation in this region were significantly reduced in wild type plants under salt stress, whereas no changes were found in RdDM mutants.Northern blot and quantitative real-time reverse transcription PCR analysis showed that the accumulation of 24-nt siRNAs was decreased in WT plants under salt stress.These results suggest that changes in the levels of the five 24-nt siRNAs regulate the AtMYB74 transcription factor via RdDM in response to salt stress.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, PR China.

No MeSH data available.


Related in: MedlinePlus

Expression of AtMYB74 and subcellular localization of AtMYB74 protein. (A) Protein sequence comparison between AtMYB74 and other plant MYB proteins. (B) qRT-PCR analysis of AtMYB74 expression in various tissues. Results were normalized to the expression of tubulin. Error bars represent SD (n = 3). R: root, S: stem, RL: rosette leaf, CL: cauline leaf, F: flower, Si: silique. (C) Tissue patterns of a 2kb putative promoter of AtMYB74-driven GUS expression in seedlings at different ages or in different tissues: (a) 7-day-old seedling, (b) euphylla, (c) lateral root primordium, (d) 2-week-old seedling, (e) flower, (f) silique, (g) seeds, (h) 7-day-old seedling treated with 150mM NaCl for 3h. Scale bars = 0.5mm. (D) Nuclear localization of AtMYB74 protein in onion epidermal cells. Images in the right column show the control plasmid expressing only GFP and those in the left column show the AtMYB74–GFP fusion protein expressed in onion epidermal cells. The cells were examined with UV fluorescence (top) and bright-field (middle) microscopy, and as a merged image (bottom) showing either the diffuse (control plasmid) or nuclear localization of the proteins. Scale bars = 20 μm. (This figure is available in colour at JXB online.)
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Figure 2: Expression of AtMYB74 and subcellular localization of AtMYB74 protein. (A) Protein sequence comparison between AtMYB74 and other plant MYB proteins. (B) qRT-PCR analysis of AtMYB74 expression in various tissues. Results were normalized to the expression of tubulin. Error bars represent SD (n = 3). R: root, S: stem, RL: rosette leaf, CL: cauline leaf, F: flower, Si: silique. (C) Tissue patterns of a 2kb putative promoter of AtMYB74-driven GUS expression in seedlings at different ages or in different tissues: (a) 7-day-old seedling, (b) euphylla, (c) lateral root primordium, (d) 2-week-old seedling, (e) flower, (f) silique, (g) seeds, (h) 7-day-old seedling treated with 150mM NaCl for 3h. Scale bars = 0.5mm. (D) Nuclear localization of AtMYB74 protein in onion epidermal cells. Images in the right column show the control plasmid expressing only GFP and those in the left column show the AtMYB74–GFP fusion protein expressed in onion epidermal cells. The cells were examined with UV fluorescence (top) and bright-field (middle) microscopy, and as a merged image (bottom) showing either the diffuse (control plasmid) or nuclear localization of the proteins. Scale bars = 20 μm. (This figure is available in colour at JXB online.)

Mentions: The full-length cDNA corresponding to the AtMYB74 mRNA is 975bp in length and encodes a putative protein of 324 amino acids. The R2 and R3 MYB domains (amino acids 13–65 and 66–117, respectively) of AtMYB74 are highly conserved with all other MYB proteins in Arabidopsis and in other plant species (Fig. 2A).


Salt-induced transcription factor MYB74 is regulated by the RNA-directed DNA methylation pathway in Arabidopsis.

Xu R, Wang Y, Zheng H, Lu W, Wu C, Huang J, Yan K, Yang G, Zheng C - J. Exp. Bot. (2015)

Expression of AtMYB74 and subcellular localization of AtMYB74 protein. (A) Protein sequence comparison between AtMYB74 and other plant MYB proteins. (B) qRT-PCR analysis of AtMYB74 expression in various tissues. Results were normalized to the expression of tubulin. Error bars represent SD (n = 3). R: root, S: stem, RL: rosette leaf, CL: cauline leaf, F: flower, Si: silique. (C) Tissue patterns of a 2kb putative promoter of AtMYB74-driven GUS expression in seedlings at different ages or in different tissues: (a) 7-day-old seedling, (b) euphylla, (c) lateral root primordium, (d) 2-week-old seedling, (e) flower, (f) silique, (g) seeds, (h) 7-day-old seedling treated with 150mM NaCl for 3h. Scale bars = 0.5mm. (D) Nuclear localization of AtMYB74 protein in onion epidermal cells. Images in the right column show the control plasmid expressing only GFP and those in the left column show the AtMYB74–GFP fusion protein expressed in onion epidermal cells. The cells were examined with UV fluorescence (top) and bright-field (middle) microscopy, and as a merged image (bottom) showing either the diffuse (control plasmid) or nuclear localization of the proteins. Scale bars = 20 μm. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4566987&req=5

Figure 2: Expression of AtMYB74 and subcellular localization of AtMYB74 protein. (A) Protein sequence comparison between AtMYB74 and other plant MYB proteins. (B) qRT-PCR analysis of AtMYB74 expression in various tissues. Results were normalized to the expression of tubulin. Error bars represent SD (n = 3). R: root, S: stem, RL: rosette leaf, CL: cauline leaf, F: flower, Si: silique. (C) Tissue patterns of a 2kb putative promoter of AtMYB74-driven GUS expression in seedlings at different ages or in different tissues: (a) 7-day-old seedling, (b) euphylla, (c) lateral root primordium, (d) 2-week-old seedling, (e) flower, (f) silique, (g) seeds, (h) 7-day-old seedling treated with 150mM NaCl for 3h. Scale bars = 0.5mm. (D) Nuclear localization of AtMYB74 protein in onion epidermal cells. Images in the right column show the control plasmid expressing only GFP and those in the left column show the AtMYB74–GFP fusion protein expressed in onion epidermal cells. The cells were examined with UV fluorescence (top) and bright-field (middle) microscopy, and as a merged image (bottom) showing either the diffuse (control plasmid) or nuclear localization of the proteins. Scale bars = 20 μm. (This figure is available in colour at JXB online.)
Mentions: The full-length cDNA corresponding to the AtMYB74 mRNA is 975bp in length and encodes a putative protein of 324 amino acids. The R2 and R3 MYB domains (amino acids 13–65 and 66–117, respectively) of AtMYB74 are highly conserved with all other MYB proteins in Arabidopsis and in other plant species (Fig. 2A).

Bottom Line: Levels of DNA methylation in this region were significantly reduced in wild type plants under salt stress, whereas no changes were found in RdDM mutants.Northern blot and quantitative real-time reverse transcription PCR analysis showed that the accumulation of 24-nt siRNAs was decreased in WT plants under salt stress.These results suggest that changes in the levels of the five 24-nt siRNAs regulate the AtMYB74 transcription factor via RdDM in response to salt stress.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, PR China.

No MeSH data available.


Related in: MedlinePlus