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Expression of Brassica napus TTG2, a regulator of trichome development, increases plant sensitivity to salt stress by suppressing the expression of auxin biosynthesis genes.

Li Q, Yin M, Li Y, Fan C, Yang Q, Wu J, Zhang C, Wang H, Zhou Y - J. Exp. Bot. (2015)

Bottom Line: In BnaA.TTG2.a.1-overexpressing Arabidopsis under salt stress, the endogenous indole-3-acetic acid (IAA) content was reduced, and the expression of two auxin biosynthesis genes, TRYPTOPHAN BIOSYNTHESIS 5 (TRP5) and YUCCA2 (YUC2), was downregulated.The results from yeast one-hybrid, electrophoretic mobility shift, and dual-luciferase reporter assays revealed that BnaA.TTG2.a.1 is able to bind to the promoters of TRP5 and YUC2.Therefore, in addition to its classical function in trichome development, our study reveals a novel role for Bna.TTG2 genes in salt stress responses.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.

No MeSH data available.


Related in: MedlinePlus

Response of BnaA.TTG2.a.1-overexpressing Arabidopsis and B. napus plants to salt stress. (A, B) Four-day-old WT and transgenic Arabidopsis plants (OE-5-2 and OE-22-1) grown on ½ MS were transferred to ½ MS (A) or ½ MS containing 75mM NaCl (B) and allowed to grow for an additional 10 d. (C, D) Fresh weight (FW) (C) and dry weight (DW) (D) of the aerial part of WT and transgenic Arabidopsis plants treated with 75mM NaCl for 10 d (n=15). (E–G) Primary root length (E), lateral root number (F), and lateral root density (lateral roots per centimetre of the primary root) (G) of WT and transgenic Arabidopsis in ½ MS or ½ MS containing 75mM NaCl for 7 d (n=16–18). (H, I) WT and 35S:BnaA.TTG2.a.1:GFP B. napus seedlings were grown with Hoagland solution (H) or Hoagland solution supplemented with 150mM NaCl (I) for 15 d. (J, K) FW (J) and DW (K) of the aerial part of WT and 35S:BnaA.TTG2.a.1:GFP transgenic B. napus plants treated with Hoagland solution or Hoagland solution with 150mM NaCl for 15 d (n=10). Bars, 10cm (H, I). Data in (C–G), (J), and (K) are means±SE from three independent experiments. Asterisks (**) indicate a significant difference between WT and respective transgenic line at the P<0.01 level of the t-test.
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Figure 3: Response of BnaA.TTG2.a.1-overexpressing Arabidopsis and B. napus plants to salt stress. (A, B) Four-day-old WT and transgenic Arabidopsis plants (OE-5-2 and OE-22-1) grown on ½ MS were transferred to ½ MS (A) or ½ MS containing 75mM NaCl (B) and allowed to grow for an additional 10 d. (C, D) Fresh weight (FW) (C) and dry weight (DW) (D) of the aerial part of WT and transgenic Arabidopsis plants treated with 75mM NaCl for 10 d (n=15). (E–G) Primary root length (E), lateral root number (F), and lateral root density (lateral roots per centimetre of the primary root) (G) of WT and transgenic Arabidopsis in ½ MS or ½ MS containing 75mM NaCl for 7 d (n=16–18). (H, I) WT and 35S:BnaA.TTG2.a.1:GFP B. napus seedlings were grown with Hoagland solution (H) or Hoagland solution supplemented with 150mM NaCl (I) for 15 d. (J, K) FW (J) and DW (K) of the aerial part of WT and 35S:BnaA.TTG2.a.1:GFP transgenic B. napus plants treated with Hoagland solution or Hoagland solution with 150mM NaCl for 15 d (n=10). Bars, 10cm (H, I). Data in (C–G), (J), and (K) are means±SE from three independent experiments. Asterisks (**) indicate a significant difference between WT and respective transgenic line at the P<0.01 level of the t-test.

Mentions: Many WRKY TFs are involved in regulating plant abiotic stress (Rushton et al., 2010), but such a role has not been reported for TTG2 genes. To explore whether BnaA.TTG2.a.1 is involved in plant responses to abiotic stress, the Arabidopsis homozygous T3 BnaA.TTG2.a.1-overexpressing lines OE-5-2 and OE-22-1 were subjected to salt stress. Without salt stress, no difference in growth between the OE and WT plants was observed (Fig. 3A). Surprisingly, the OE plants displayed dramatic growth inhibition (Fig. 3B) at 75mM NaCl with significantly reduced fresh and dry weights (Fig. 3C, D) compared with the WT plants. Both the number and density of the lateral roots in OE plants were reduced compared with those of WT in the presence of 75mM NaCl, although the OE roots exhibited a similar primary root length as the WT roots (Fig. 3E–G).


Expression of Brassica napus TTG2, a regulator of trichome development, increases plant sensitivity to salt stress by suppressing the expression of auxin biosynthesis genes.

Li Q, Yin M, Li Y, Fan C, Yang Q, Wu J, Zhang C, Wang H, Zhou Y - J. Exp. Bot. (2015)

Response of BnaA.TTG2.a.1-overexpressing Arabidopsis and B. napus plants to salt stress. (A, B) Four-day-old WT and transgenic Arabidopsis plants (OE-5-2 and OE-22-1) grown on ½ MS were transferred to ½ MS (A) or ½ MS containing 75mM NaCl (B) and allowed to grow for an additional 10 d. (C, D) Fresh weight (FW) (C) and dry weight (DW) (D) of the aerial part of WT and transgenic Arabidopsis plants treated with 75mM NaCl for 10 d (n=15). (E–G) Primary root length (E), lateral root number (F), and lateral root density (lateral roots per centimetre of the primary root) (G) of WT and transgenic Arabidopsis in ½ MS or ½ MS containing 75mM NaCl for 7 d (n=16–18). (H, I) WT and 35S:BnaA.TTG2.a.1:GFP B. napus seedlings were grown with Hoagland solution (H) or Hoagland solution supplemented with 150mM NaCl (I) for 15 d. (J, K) FW (J) and DW (K) of the aerial part of WT and 35S:BnaA.TTG2.a.1:GFP transgenic B. napus plants treated with Hoagland solution or Hoagland solution with 150mM NaCl for 15 d (n=10). Bars, 10cm (H, I). Data in (C–G), (J), and (K) are means±SE from three independent experiments. Asterisks (**) indicate a significant difference between WT and respective transgenic line at the P<0.01 level of the t-test.
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Figure 3: Response of BnaA.TTG2.a.1-overexpressing Arabidopsis and B. napus plants to salt stress. (A, B) Four-day-old WT and transgenic Arabidopsis plants (OE-5-2 and OE-22-1) grown on ½ MS were transferred to ½ MS (A) or ½ MS containing 75mM NaCl (B) and allowed to grow for an additional 10 d. (C, D) Fresh weight (FW) (C) and dry weight (DW) (D) of the aerial part of WT and transgenic Arabidopsis plants treated with 75mM NaCl for 10 d (n=15). (E–G) Primary root length (E), lateral root number (F), and lateral root density (lateral roots per centimetre of the primary root) (G) of WT and transgenic Arabidopsis in ½ MS or ½ MS containing 75mM NaCl for 7 d (n=16–18). (H, I) WT and 35S:BnaA.TTG2.a.1:GFP B. napus seedlings were grown with Hoagland solution (H) or Hoagland solution supplemented with 150mM NaCl (I) for 15 d. (J, K) FW (J) and DW (K) of the aerial part of WT and 35S:BnaA.TTG2.a.1:GFP transgenic B. napus plants treated with Hoagland solution or Hoagland solution with 150mM NaCl for 15 d (n=10). Bars, 10cm (H, I). Data in (C–G), (J), and (K) are means±SE from three independent experiments. Asterisks (**) indicate a significant difference between WT and respective transgenic line at the P<0.01 level of the t-test.
Mentions: Many WRKY TFs are involved in regulating plant abiotic stress (Rushton et al., 2010), but such a role has not been reported for TTG2 genes. To explore whether BnaA.TTG2.a.1 is involved in plant responses to abiotic stress, the Arabidopsis homozygous T3 BnaA.TTG2.a.1-overexpressing lines OE-5-2 and OE-22-1 were subjected to salt stress. Without salt stress, no difference in growth between the OE and WT plants was observed (Fig. 3A). Surprisingly, the OE plants displayed dramatic growth inhibition (Fig. 3B) at 75mM NaCl with significantly reduced fresh and dry weights (Fig. 3C, D) compared with the WT plants. Both the number and density of the lateral roots in OE plants were reduced compared with those of WT in the presence of 75mM NaCl, although the OE roots exhibited a similar primary root length as the WT roots (Fig. 3E–G).

Bottom Line: In BnaA.TTG2.a.1-overexpressing Arabidopsis under salt stress, the endogenous indole-3-acetic acid (IAA) content was reduced, and the expression of two auxin biosynthesis genes, TRYPTOPHAN BIOSYNTHESIS 5 (TRP5) and YUCCA2 (YUC2), was downregulated.The results from yeast one-hybrid, electrophoretic mobility shift, and dual-luciferase reporter assays revealed that BnaA.TTG2.a.1 is able to bind to the promoters of TRP5 and YUC2.Therefore, in addition to its classical function in trichome development, our study reveals a novel role for Bna.TTG2 genes in salt stress responses.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.

No MeSH data available.


Related in: MedlinePlus