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Comprehensive transcript profiling of two grapevine rootstock genotypes contrasting in drought susceptibility links the phenylpropanoid pathway to enhanced tolerance.

Corso M, Vannozzi A, Maza E, Vitulo N, Meggio F, Pitacco A, Telatin A, D'Angelo M, Feltrin E, Negri AS, Prinsi B, Valle G, Ramina A, Bouzayen M, Bonghi C, Lucchin M - J. Exp. Bot. (2015)

Bottom Line: Upon stress, M4 roots and leaves showed a higher induction of resveratrol and flavonoid biosynthetic genes, respectively.The higher expression of VvSTS genes in M4, confirmed by the accumulation of higher levels of resveratrol in M4 roots compared with 101.14, was coupled to an up-regulation of several VvWRKY transcription factors.It is proposed that the elevated synthesis of resveratrol in M4 roots upon water stress could enhance the plant's ability to cope with the oxidative stress usually associated with water deficit.

View Article: PubMed Central - PubMed

Affiliation: Department of Agronomy, Food, Natural resources, Animals and Environment (DAFNAE), University of Padova Agripolis, 35020 Legnaro, Italy Centro Interdipartimentale per la Ricerca in Viticoltura ed Enologia (CIRVE), Via XXVIII Aprile, 14-31015 Conegliano (TV), Italy.

No MeSH data available.


Related in: MedlinePlus

Hypothetical model summarizing the events occurring in leaves and roots of M4 and 101.14 upon WS. Grey and red boxes list molecular responses to WS that are common to both genotypes and M4 specific, respectively. White boxes report physiological events associated with WS occurring in root (lateral roots growth and water suction from soil) and leaves (transpiration, E; net-assimilation, An; photosynthesis) of both genotypes. Dashed lines indicate the lower impact of WS on the physiological events in roots and leaves observed in M4.
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Figure 7: Hypothetical model summarizing the events occurring in leaves and roots of M4 and 101.14 upon WS. Grey and red boxes list molecular responses to WS that are common to both genotypes and M4 specific, respectively. White boxes report physiological events associated with WS occurring in root (lateral roots growth and water suction from soil) and leaves (transpiration, E; net-assimilation, An; photosynthesis) of both genotypes. Dashed lines indicate the lower impact of WS on the physiological events in roots and leaves observed in M4.

Mentions: In conclusion, this study provides a comprehensive description of the transcriptomic responses to drought in roots and leaves of two grapevine genotypes with different tolerances to WS. In contrast to previous studies (Cramer et al., 2007; Tattersall et al., 2007; Tillett et al., 2011), responses to WS that are common to susceptible and tolerant plants were not considered, but rather the focus was on genes whose expression is strictly related to the tolerant genotype. On this basis, it is proposed that the drought tolerance displayed by the M4 genotype could be associated with an enhanced capacity to scavenge ROS produced under stress conditions and that this may be mainly conferred by structural variations in the promoter of genes involved in stilbene biosynthesis (Fig. 7). In water-stressed M4 plants, the higher ROS detoxification ability could allow lateral root growth to be maintained, resulting in higher water uptake capacity from the soil, as previously observed by Tsukagoshi (2012). Likewise, at the leaf level, a higher E in the drought-tolerant genotype would promote active plant growth and photosynthesis. In contrast, in 101.14, where the oxidative stress is not efficiently counteracted, the functionality of roots and leaves is strongly impaired (Fig. 7).


Comprehensive transcript profiling of two grapevine rootstock genotypes contrasting in drought susceptibility links the phenylpropanoid pathway to enhanced tolerance.

Corso M, Vannozzi A, Maza E, Vitulo N, Meggio F, Pitacco A, Telatin A, D'Angelo M, Feltrin E, Negri AS, Prinsi B, Valle G, Ramina A, Bouzayen M, Bonghi C, Lucchin M - J. Exp. Bot. (2015)

Hypothetical model summarizing the events occurring in leaves and roots of M4 and 101.14 upon WS. Grey and red boxes list molecular responses to WS that are common to both genotypes and M4 specific, respectively. White boxes report physiological events associated with WS occurring in root (lateral roots growth and water suction from soil) and leaves (transpiration, E; net-assimilation, An; photosynthesis) of both genotypes. Dashed lines indicate the lower impact of WS on the physiological events in roots and leaves observed in M4.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4566973&req=5

Figure 7: Hypothetical model summarizing the events occurring in leaves and roots of M4 and 101.14 upon WS. Grey and red boxes list molecular responses to WS that are common to both genotypes and M4 specific, respectively. White boxes report physiological events associated with WS occurring in root (lateral roots growth and water suction from soil) and leaves (transpiration, E; net-assimilation, An; photosynthesis) of both genotypes. Dashed lines indicate the lower impact of WS on the physiological events in roots and leaves observed in M4.
Mentions: In conclusion, this study provides a comprehensive description of the transcriptomic responses to drought in roots and leaves of two grapevine genotypes with different tolerances to WS. In contrast to previous studies (Cramer et al., 2007; Tattersall et al., 2007; Tillett et al., 2011), responses to WS that are common to susceptible and tolerant plants were not considered, but rather the focus was on genes whose expression is strictly related to the tolerant genotype. On this basis, it is proposed that the drought tolerance displayed by the M4 genotype could be associated with an enhanced capacity to scavenge ROS produced under stress conditions and that this may be mainly conferred by structural variations in the promoter of genes involved in stilbene biosynthesis (Fig. 7). In water-stressed M4 plants, the higher ROS detoxification ability could allow lateral root growth to be maintained, resulting in higher water uptake capacity from the soil, as previously observed by Tsukagoshi (2012). Likewise, at the leaf level, a higher E in the drought-tolerant genotype would promote active plant growth and photosynthesis. In contrast, in 101.14, where the oxidative stress is not efficiently counteracted, the functionality of roots and leaves is strongly impaired (Fig. 7).

Bottom Line: Upon stress, M4 roots and leaves showed a higher induction of resveratrol and flavonoid biosynthetic genes, respectively.The higher expression of VvSTS genes in M4, confirmed by the accumulation of higher levels of resveratrol in M4 roots compared with 101.14, was coupled to an up-regulation of several VvWRKY transcription factors.It is proposed that the elevated synthesis of resveratrol in M4 roots upon water stress could enhance the plant's ability to cope with the oxidative stress usually associated with water deficit.

View Article: PubMed Central - PubMed

Affiliation: Department of Agronomy, Food, Natural resources, Animals and Environment (DAFNAE), University of Padova Agripolis, 35020 Legnaro, Italy Centro Interdipartimentale per la Ricerca in Viticoltura ed Enologia (CIRVE), Via XXVIII Aprile, 14-31015 Conegliano (TV), Italy.

No MeSH data available.


Related in: MedlinePlus