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Coriandrum sativum L. seed extract mitigates lipotoxicity in RAW 264.7 cells and prevents atherogenic changes in rats.

Patel D, Desai S, Gajaria T, Devkar R, Ramachandran AV - EXCLI J (2013)

Bottom Line: Copper mediated cell free oxidation of LDL accounted for elevated indices of malondialdehyde (MDA), lipid hydroperoxide (LHP)and protein carbonyl (PC) and a progressive increment in conjugate diene (CD) levels whereas, reverse set of changes were recorded in presence of CS extract.Cell mediated LDL oxidation (using RAW 264.7 cells) accounted for lowered MDA production and oxidized LDL (Ox-LDL) mediated cell death in presence of CS extract and the same was attributed to its potent antioxidant and free radical scavenging potentials.The same was further validated with immunostaining of cell adhesion molecules and hematoxylin and eosin (HXE) staining.

View Article: PubMed Central - PubMed

Affiliation: Division of Phytotherapeutics and Metabolic Endocrinology, Department of Zoology, Faculty of Science, The M. S. University of Baroda, Gujarat, India.

ABSTRACT
This study was designed to assess the efficacy of Coriandrum sativum L. (CS) in preventing in vitro low density lipoprotein (LDL) oxidation mediated macrophage modification. Further, an in vivo study was also conducted to confirm upon the efficacy of CS seed extract in alleviating pathophysiological alterations of high cholesterol diet induced atherosclerosis in rats. Copper mediated cell free oxidation of LDL accounted for elevated indices of malondialdehyde (MDA), lipid hydroperoxide (LHP)and protein carbonyl (PC) and a progressive increment in conjugate diene (CD) levels whereas, reverse set of changes were recorded in presence of CS extract. Cell mediated LDL oxidation (using RAW 264.7 cells) accounted for lowered MDA production and oxidized LDL (Ox-LDL) mediated cell death in presence of CS extract and the same was attributed to its potent antioxidant and free radical scavenging potentials. High cholesterol fed atherogenic rats showed elevated lipid indices, evidences of LDL oxidation, plaque formation in thoracic aorta. The same was further validated with immunostaining of cell adhesion molecules and hematoxylin and eosin (HXE) staining. However, co-supplementation of CS to atherogenic rats recorded significant lowering of the above mentioned parameters further strengthening the claim that CS extract is instrumental in preventing onset and progression of atherosclerosis.

No MeSH data available.


Related in: MedlinePlus

Effect of CS on lipid and lipoprotein profiles of control (NC), atherogenic diet fed (ATH) and ATH+CS supplemented rats
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Figure 11: Effect of CS on lipid and lipoprotein profiles of control (NC), atherogenic diet fed (ATH) and ATH+CS supplemented rats

Mentions: There was significant (p<0.001) elevation in serum TC, TG, VLDL and LDL levels and, a decrement in HDL level recorded in ATH rats as compared to the control rats. However, ATH+ CS rats showed significant (p<0.001) decrement in TC, TG, VLDL and LDL levels and increment in HDL level as compared to ATH group. There was significant (p<0.001) increment in MDA level the LDL isolated from ATH rats as compared to the control rats. However, ATH+CS treated rats recorded significant (p<0.001) decrement in MDA level (Figures 11(Fig. 11) and 12(Fig. 12)).


Coriandrum sativum L. seed extract mitigates lipotoxicity in RAW 264.7 cells and prevents atherogenic changes in rats.

Patel D, Desai S, Gajaria T, Devkar R, Ramachandran AV - EXCLI J (2013)

Effect of CS on lipid and lipoprotein profiles of control (NC), atherogenic diet fed (ATH) and ATH+CS supplemented rats
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4566908&req=5

Figure 11: Effect of CS on lipid and lipoprotein profiles of control (NC), atherogenic diet fed (ATH) and ATH+CS supplemented rats
Mentions: There was significant (p<0.001) elevation in serum TC, TG, VLDL and LDL levels and, a decrement in HDL level recorded in ATH rats as compared to the control rats. However, ATH+ CS rats showed significant (p<0.001) decrement in TC, TG, VLDL and LDL levels and increment in HDL level as compared to ATH group. There was significant (p<0.001) increment in MDA level the LDL isolated from ATH rats as compared to the control rats. However, ATH+CS treated rats recorded significant (p<0.001) decrement in MDA level (Figures 11(Fig. 11) and 12(Fig. 12)).

Bottom Line: Copper mediated cell free oxidation of LDL accounted for elevated indices of malondialdehyde (MDA), lipid hydroperoxide (LHP)and protein carbonyl (PC) and a progressive increment in conjugate diene (CD) levels whereas, reverse set of changes were recorded in presence of CS extract.Cell mediated LDL oxidation (using RAW 264.7 cells) accounted for lowered MDA production and oxidized LDL (Ox-LDL) mediated cell death in presence of CS extract and the same was attributed to its potent antioxidant and free radical scavenging potentials.The same was further validated with immunostaining of cell adhesion molecules and hematoxylin and eosin (HXE) staining.

View Article: PubMed Central - PubMed

Affiliation: Division of Phytotherapeutics and Metabolic Endocrinology, Department of Zoology, Faculty of Science, The M. S. University of Baroda, Gujarat, India.

ABSTRACT
This study was designed to assess the efficacy of Coriandrum sativum L. (CS) in preventing in vitro low density lipoprotein (LDL) oxidation mediated macrophage modification. Further, an in vivo study was also conducted to confirm upon the efficacy of CS seed extract in alleviating pathophysiological alterations of high cholesterol diet induced atherosclerosis in rats. Copper mediated cell free oxidation of LDL accounted for elevated indices of malondialdehyde (MDA), lipid hydroperoxide (LHP)and protein carbonyl (PC) and a progressive increment in conjugate diene (CD) levels whereas, reverse set of changes were recorded in presence of CS extract. Cell mediated LDL oxidation (using RAW 264.7 cells) accounted for lowered MDA production and oxidized LDL (Ox-LDL) mediated cell death in presence of CS extract and the same was attributed to its potent antioxidant and free radical scavenging potentials. High cholesterol fed atherogenic rats showed elevated lipid indices, evidences of LDL oxidation, plaque formation in thoracic aorta. The same was further validated with immunostaining of cell adhesion molecules and hematoxylin and eosin (HXE) staining. However, co-supplementation of CS to atherogenic rats recorded significant lowering of the above mentioned parameters further strengthening the claim that CS extract is instrumental in preventing onset and progression of atherosclerosis.

No MeSH data available.


Related in: MedlinePlus