Limits...
Hepatoprotective and Pancreatoprotective Properties of the Ethanolic Extract of Nigerian Propolis.

Babatunde IR, Abdulbasit A, Oladayo MI, Olasile OI, Olamide FR, Gbolahan BW - J Intercult Ethnopharmacol (2015)

Bottom Line: In the present study, we investigated the effects of the ethanolic extract of Nigerian propolis (N. propolis) on markers of oxidative stress, histology of the liver and pancreas and glycaemia in alloxan-induced diabetic rats.Serum malondialdehyde levels was reduced while superoxide dismutase levels were elevated in the N. propolis group; these levels were converse in the diabetic group, these differences are statistically significant (P<0.05) when compared with the control.Histologically, there was improvement in the treated group compared to the untreated group.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, University of Ilorin, Ilorin, Nigeria.

ABSTRACT

Objective: Increased oxidative stress is associated with the progression of diabetic mellitus. In the present study, we investigated the effects of the ethanolic extract of Nigerian propolis (N. propolis) on markers of oxidative stress, histology of the liver and pancreas and glycaemia in alloxan-induced diabetic rats.

Materials and methods: Alloxan-induced hyperglycemic Wistar rats were treated with either metformin (150 mg/kg/d) or N. propolis (200 mg/kg/d and 300 mg/kg/d) for 28 days. At the end of the treatment period, the rats were sacrificed; blood was collected for biochemical analysis while their pancreases and liver were excised and processed for histological studies.

Results: Serum oxidative stress markers and blood glucose concentration were compared between the treated and control rats. In contrast to the non-treated diabetic rats, blood glucose concentration were not significantly different between treated rats and control (P < 0.05) at 28 days of treatment with N. propolis and metformin. Serum malondialdehyde levels was reduced while superoxide dismutase levels were elevated in the N. propolis group; these levels were converse in the diabetic group, these differences are statistically significant (P<0.05) when compared with the control. Histologically, there was improvement in the treated group compared to the untreated group.

Conclusion: These findings suggest that the N. propolis confers protection against hyperglycemia-induced oxidative stress in both liver and pancreas of adult Wistar rats.

No MeSH data available.


Related in: MedlinePlus

Pancreas of rats at 28 days of treatment. (a) Control group: The morphology of the pancreas is normal as indicated by intact islet of Langerhans cells (I) and acinar cells (arrows). (b) Diabetic group: There are degenerative and lytic changes in the islet of Langerhans of pancreas. (c) Diabetic + metformin: The pancreas has normal morphology (I show islet cells, arrow show acinar cells). (d) Diabetic + 200 mg/kg of Nigerian propolis; Pancreas morphology shows improvement comparable to control. (e) Diabetic + 300 mg/kg of Nigerian propolis. Pancreas morphology is comparable to control (H and E, 400×)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4566782&req=5

Figure 6: Pancreas of rats at 28 days of treatment. (a) Control group: The morphology of the pancreas is normal as indicated by intact islet of Langerhans cells (I) and acinar cells (arrows). (b) Diabetic group: There are degenerative and lytic changes in the islet of Langerhans of pancreas. (c) Diabetic + metformin: The pancreas has normal morphology (I show islet cells, arrow show acinar cells). (d) Diabetic + 200 mg/kg of Nigerian propolis; Pancreas morphology shows improvement comparable to control. (e) Diabetic + 300 mg/kg of Nigerian propolis. Pancreas morphology is comparable to control (H and E, 400×)

Mentions: The section of rat pancreas from normal control group reveals normal pancreatic acini and islet cells, a similar result was found in the group treated with N. propolis and metformin. Alloxan-diabetic rats demonstrate degenerative and lytic changes in the islet of Langerhans of the pancreas as seen in Figure 6a-e.


Hepatoprotective and Pancreatoprotective Properties of the Ethanolic Extract of Nigerian Propolis.

Babatunde IR, Abdulbasit A, Oladayo MI, Olasile OI, Olamide FR, Gbolahan BW - J Intercult Ethnopharmacol (2015)

Pancreas of rats at 28 days of treatment. (a) Control group: The morphology of the pancreas is normal as indicated by intact islet of Langerhans cells (I) and acinar cells (arrows). (b) Diabetic group: There are degenerative and lytic changes in the islet of Langerhans of pancreas. (c) Diabetic + metformin: The pancreas has normal morphology (I show islet cells, arrow show acinar cells). (d) Diabetic + 200 mg/kg of Nigerian propolis; Pancreas morphology shows improvement comparable to control. (e) Diabetic + 300 mg/kg of Nigerian propolis. Pancreas morphology is comparable to control (H and E, 400×)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4566782&req=5

Figure 6: Pancreas of rats at 28 days of treatment. (a) Control group: The morphology of the pancreas is normal as indicated by intact islet of Langerhans cells (I) and acinar cells (arrows). (b) Diabetic group: There are degenerative and lytic changes in the islet of Langerhans of pancreas. (c) Diabetic + metformin: The pancreas has normal morphology (I show islet cells, arrow show acinar cells). (d) Diabetic + 200 mg/kg of Nigerian propolis; Pancreas morphology shows improvement comparable to control. (e) Diabetic + 300 mg/kg of Nigerian propolis. Pancreas morphology is comparable to control (H and E, 400×)
Mentions: The section of rat pancreas from normal control group reveals normal pancreatic acini and islet cells, a similar result was found in the group treated with N. propolis and metformin. Alloxan-diabetic rats demonstrate degenerative and lytic changes in the islet of Langerhans of the pancreas as seen in Figure 6a-e.

Bottom Line: In the present study, we investigated the effects of the ethanolic extract of Nigerian propolis (N. propolis) on markers of oxidative stress, histology of the liver and pancreas and glycaemia in alloxan-induced diabetic rats.Serum malondialdehyde levels was reduced while superoxide dismutase levels were elevated in the N. propolis group; these levels were converse in the diabetic group, these differences are statistically significant (P<0.05) when compared with the control.Histologically, there was improvement in the treated group compared to the untreated group.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, University of Ilorin, Ilorin, Nigeria.

ABSTRACT

Objective: Increased oxidative stress is associated with the progression of diabetic mellitus. In the present study, we investigated the effects of the ethanolic extract of Nigerian propolis (N. propolis) on markers of oxidative stress, histology of the liver and pancreas and glycaemia in alloxan-induced diabetic rats.

Materials and methods: Alloxan-induced hyperglycemic Wistar rats were treated with either metformin (150 mg/kg/d) or N. propolis (200 mg/kg/d and 300 mg/kg/d) for 28 days. At the end of the treatment period, the rats were sacrificed; blood was collected for biochemical analysis while their pancreases and liver were excised and processed for histological studies.

Results: Serum oxidative stress markers and blood glucose concentration were compared between the treated and control rats. In contrast to the non-treated diabetic rats, blood glucose concentration were not significantly different between treated rats and control (P < 0.05) at 28 days of treatment with N. propolis and metformin. Serum malondialdehyde levels was reduced while superoxide dismutase levels were elevated in the N. propolis group; these levels were converse in the diabetic group, these differences are statistically significant (P<0.05) when compared with the control. Histologically, there was improvement in the treated group compared to the untreated group.

Conclusion: These findings suggest that the N. propolis confers protection against hyperglycemia-induced oxidative stress in both liver and pancreas of adult Wistar rats.

No MeSH data available.


Related in: MedlinePlus