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Protective effect of N-acetylcysteine against ethanol-induced gastric ulcer: A pharmacological assessment in mice.

Jaccob AA - J Intercult Ethnopharmacol (2015)

Bottom Line: Then 3 h after, all animals were sacrificed then consequently the stomachs were excised for examination.NAC administration at the tested doses showed a dose-related potent gastro-protective effect with significant increase in curative ratio, PH of gastric juice and mucus content viscosity seen with the highest dose of NAC and it is comparable with that observed in ranitidine group.The present findings demonstrate that, oral NAC shows significant gastro-protective effects comparable to ranitidine confirmed by anti-secretory, cytoprotective, histological and biochemical data, but the molecular mechanisms behind such protection are complex.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology and Toxicology, College of Pharmacy, University of Basra, Basra, Iraq.

ABSTRACT

Aim: Since there is an increasing need for gastric ulcer therapies with optimum benefit-risk profile. This study was conducted to investigate gastro-protective effects of N-acetylcysteine (NAC) against ethanol-induced gastric ulcer models in mice.

Materials and methods: A total of 41 mice were allocated into six groups consisted of 7 mice each. Groups 1 (normal control) and 2 (ulcer control) received distilled water at a dose of 10 ml/kg, groups 3, 4 and 5 were given NAC at doses 100, 300 and 500 mg/kg, respectively, and the 6(th) group received ranitidine (50 mg/kg). All drugs administered orally once daily for 7 days, on the 8(th) day absolute ethanol (7 ml/kg) was administrated orally to all mice to induce the acute ulcer except normal control group. Then 3 h after, all animals were sacrificed then consequently the stomachs were excised for examination.

Results: NAC administration at the tested doses showed a dose-related potent gastro-protective effect with significant increase in curative ratio, PH of gastric juice and mucus content viscosity seen with the highest dose of NAC and it is comparable with that observed in ranitidine group.

Conclusion: The present findings demonstrate that, oral NAC shows significant gastro-protective effects comparable to ranitidine confirmed by anti-secretory, cytoprotective, histological and biochemical data, but the molecular mechanisms behind such protection are complex.

No MeSH data available.


Related in: MedlinePlus

Curative ratio of different doses of NAC and ranitidine on gastric ulcer in mice (n = 7), ANOVA: Values with different letters a, b, c, P < 0.05 versus normal control, ulcer control, N-acetylcysteine (NAC) 100 mg/kg, NAC 300 mg/kg and NAC 500 mg/kg respectively
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Figure 4: Curative ratio of different doses of NAC and ranitidine on gastric ulcer in mice (n = 7), ANOVA: Values with different letters a, b, c, P < 0.05 versus normal control, ulcer control, N-acetylcysteine (NAC) 100 mg/kg, NAC 300 mg/kg and NAC 500 mg/kg respectively

Mentions: Orally administered absolute ethanol induced severe gastric mucosal lesion (lesion area = 24.5 ± 0.85); NAC at the tested doses (100, 300 and 500 mg/kg), showed a dose-related significant protective effect against gastric lesions, with significant reduction in the ulcer size achieved with the highest dose of NAC, and it is comparable with that observed in ranitidine group [Figure 3]. Figure 4 demonstrates both doses of NAC (300 and 500 mg/kg) exhibited marked increase in curative ratio, compared with that observed with ulcer control group; such increment were relatively comparable to that observed in ranitidine (as reference drug) treated group.


Protective effect of N-acetylcysteine against ethanol-induced gastric ulcer: A pharmacological assessment in mice.

Jaccob AA - J Intercult Ethnopharmacol (2015)

Curative ratio of different doses of NAC and ranitidine on gastric ulcer in mice (n = 7), ANOVA: Values with different letters a, b, c, P < 0.05 versus normal control, ulcer control, N-acetylcysteine (NAC) 100 mg/kg, NAC 300 mg/kg and NAC 500 mg/kg respectively
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4566772&req=5

Figure 4: Curative ratio of different doses of NAC and ranitidine on gastric ulcer in mice (n = 7), ANOVA: Values with different letters a, b, c, P < 0.05 versus normal control, ulcer control, N-acetylcysteine (NAC) 100 mg/kg, NAC 300 mg/kg and NAC 500 mg/kg respectively
Mentions: Orally administered absolute ethanol induced severe gastric mucosal lesion (lesion area = 24.5 ± 0.85); NAC at the tested doses (100, 300 and 500 mg/kg), showed a dose-related significant protective effect against gastric lesions, with significant reduction in the ulcer size achieved with the highest dose of NAC, and it is comparable with that observed in ranitidine group [Figure 3]. Figure 4 demonstrates both doses of NAC (300 and 500 mg/kg) exhibited marked increase in curative ratio, compared with that observed with ulcer control group; such increment were relatively comparable to that observed in ranitidine (as reference drug) treated group.

Bottom Line: Then 3 h after, all animals were sacrificed then consequently the stomachs were excised for examination.NAC administration at the tested doses showed a dose-related potent gastro-protective effect with significant increase in curative ratio, PH of gastric juice and mucus content viscosity seen with the highest dose of NAC and it is comparable with that observed in ranitidine group.The present findings demonstrate that, oral NAC shows significant gastro-protective effects comparable to ranitidine confirmed by anti-secretory, cytoprotective, histological and biochemical data, but the molecular mechanisms behind such protection are complex.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology and Toxicology, College of Pharmacy, University of Basra, Basra, Iraq.

ABSTRACT

Aim: Since there is an increasing need for gastric ulcer therapies with optimum benefit-risk profile. This study was conducted to investigate gastro-protective effects of N-acetylcysteine (NAC) against ethanol-induced gastric ulcer models in mice.

Materials and methods: A total of 41 mice were allocated into six groups consisted of 7 mice each. Groups 1 (normal control) and 2 (ulcer control) received distilled water at a dose of 10 ml/kg, groups 3, 4 and 5 were given NAC at doses 100, 300 and 500 mg/kg, respectively, and the 6(th) group received ranitidine (50 mg/kg). All drugs administered orally once daily for 7 days, on the 8(th) day absolute ethanol (7 ml/kg) was administrated orally to all mice to induce the acute ulcer except normal control group. Then 3 h after, all animals were sacrificed then consequently the stomachs were excised for examination.

Results: NAC administration at the tested doses showed a dose-related potent gastro-protective effect with significant increase in curative ratio, PH of gastric juice and mucus content viscosity seen with the highest dose of NAC and it is comparable with that observed in ranitidine group.

Conclusion: The present findings demonstrate that, oral NAC shows significant gastro-protective effects comparable to ranitidine confirmed by anti-secretory, cytoprotective, histological and biochemical data, but the molecular mechanisms behind such protection are complex.

No MeSH data available.


Related in: MedlinePlus