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Guaiazulene biochemical activity and cytotoxic and genotoxic effects on rat neuron and N2a neuroblastom cells.

Togar B, Turkez H, Hacimuftuoglu A, Tatar A, Geyikoglu F - J Intercult Ethnopharmacol (2015)

Bottom Line: The results indicated that GYZ have anti-proliferative activity suppressing the proliferation of neuron and N2a-NB cells at high doses.In addition, GYZ treatments at higher doses led to decreases of TAC levels and increases of TOS levels in neuron and N2a-NB cells.On the other hand, the mean values of the total scores of cells showing DNA damage were not found different from the control values.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey.

ABSTRACT

Aim: Neuroblastoma (NB)cells are often used in cancer researches such as glioblastoma cells since they have the potential of high mitotic activity, nuclear pleomorphism, and tumor necrosis. Guaiazulene (GYZ 1,4-dimethyl-7-isopropylazulene)is present in several essential oils of medicinal and aromatic plants. Many studies have reported the cytotoxic effect of GYZ; however, there are no studies that compare such effects between cancer cell lines and normal human cells after treatment with GYZ.

Materials and methods: In this study, we aimed to describe in vitro antiproliferative and/or cytotoxic properties (by 3-[4,5 dimetylthiazol -2-yl]-2,5 diphenlytetrazolium bromide [MTT] test), oxidative effects (by total antioxidant capacity [TAC] and total oxidative stress [TOS] analysis)and genotoxic damage potentials (by single cell gel electrophoresis)of GYZ.

Result: The results indicated that GYZ have anti-proliferative activity suppressing the proliferation of neuron and N2a-NB cells at high doses. In addition, GYZ treatments at higher doses led to decreases of TAC levels and increases of TOS levels in neuron and N2a-NB cells. On the other hand, the mean values of the total scores of cells showing DNA damage were not found different from the control values.

Conclusion: From this study, it is observed that GYZ has in vitro cytotoxic activity against neuron and N2a-NB cells.

No MeSH data available.


Related in: MedlinePlus

Effect of varying concentrations of GYZ on inducing DNA damage in vitro for 24 h. The results are given as the means ± standard deviation from six independent experiments. Compared with control, *P > 0.05
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Figure 3: Effect of varying concentrations of GYZ on inducing DNA damage in vitro for 24 h. The results are given as the means ± standard deviation from six independent experiments. Compared with control, *P > 0.05

Mentions: The induction of DNA damage in rat neuron and N2a-NB cells after exposition to GYZ was investigated using SCGE assay. The obtained results are presented as total damage score in Figure 3. None of the tested GYZ concentrations produced genotoxic effect after 24 h treatments in rat neuron and N2a-NB cells. The scoring criteria for determining DNA damage levels using comet formations in cultured neurons treated with the compounds were shown in Figure 4.


Guaiazulene biochemical activity and cytotoxic and genotoxic effects on rat neuron and N2a neuroblastom cells.

Togar B, Turkez H, Hacimuftuoglu A, Tatar A, Geyikoglu F - J Intercult Ethnopharmacol (2015)

Effect of varying concentrations of GYZ on inducing DNA damage in vitro for 24 h. The results are given as the means ± standard deviation from six independent experiments. Compared with control, *P > 0.05
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4566767&req=5

Figure 3: Effect of varying concentrations of GYZ on inducing DNA damage in vitro for 24 h. The results are given as the means ± standard deviation from six independent experiments. Compared with control, *P > 0.05
Mentions: The induction of DNA damage in rat neuron and N2a-NB cells after exposition to GYZ was investigated using SCGE assay. The obtained results are presented as total damage score in Figure 3. None of the tested GYZ concentrations produced genotoxic effect after 24 h treatments in rat neuron and N2a-NB cells. The scoring criteria for determining DNA damage levels using comet formations in cultured neurons treated with the compounds were shown in Figure 4.

Bottom Line: The results indicated that GYZ have anti-proliferative activity suppressing the proliferation of neuron and N2a-NB cells at high doses.In addition, GYZ treatments at higher doses led to decreases of TAC levels and increases of TOS levels in neuron and N2a-NB cells.On the other hand, the mean values of the total scores of cells showing DNA damage were not found different from the control values.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey.

ABSTRACT

Aim: Neuroblastoma (NB)cells are often used in cancer researches such as glioblastoma cells since they have the potential of high mitotic activity, nuclear pleomorphism, and tumor necrosis. Guaiazulene (GYZ 1,4-dimethyl-7-isopropylazulene)is present in several essential oils of medicinal and aromatic plants. Many studies have reported the cytotoxic effect of GYZ; however, there are no studies that compare such effects between cancer cell lines and normal human cells after treatment with GYZ.

Materials and methods: In this study, we aimed to describe in vitro antiproliferative and/or cytotoxic properties (by 3-[4,5 dimetylthiazol -2-yl]-2,5 diphenlytetrazolium bromide [MTT] test), oxidative effects (by total antioxidant capacity [TAC] and total oxidative stress [TOS] analysis)and genotoxic damage potentials (by single cell gel electrophoresis)of GYZ.

Result: The results indicated that GYZ have anti-proliferative activity suppressing the proliferation of neuron and N2a-NB cells at high doses. In addition, GYZ treatments at higher doses led to decreases of TAC levels and increases of TOS levels in neuron and N2a-NB cells. On the other hand, the mean values of the total scores of cells showing DNA damage were not found different from the control values.

Conclusion: From this study, it is observed that GYZ has in vitro cytotoxic activity against neuron and N2a-NB cells.

No MeSH data available.


Related in: MedlinePlus