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Evaluation of the antioxidant activity and the healing action of the ethanol extract of Calotropis procera bark against surgical wounds.

Tsala DE, Nga N, Thiery BN, Bienvenue MT, Theophile D - J Intercult Ethnopharmacol (2015)

Bottom Line: It was found to inhibit DPPH and deoxyribose oxidation (IC50 = 24.24 and 5.40 respectively).In vivo study demonstrated a significant reduction in the epithelialization time (P < 0.001) to 17-18 days in normal and dexamethasone treated rats following the ethanolic extract of the bark of C. procera application.The same extract also significantly increased the breaking strength in dexamethasone treated rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Faculty of Science, University of Maroua, Maroua-Cameroon.

ABSTRACT
The objective of the present study was to evaluate the antioxidant potential and the wound healing effect of the ethanolic extract of the bark of Calotropis procera. The antioxidant study was evaluated in vitro, using 2,2-diphenylpicrylhydrazyl (DPPH) and deoxyribose degradation assays. Wound healing was studied using excision and incision wound on normal and dexamethasone-suppressed wound healing rodent models. Alkaloids, flavonoids, proteins and phenols were screened in the extract used whereas saponins and true tannins were absent. The extract contains only 12.5 gallic acid equivalent and 399.54 rutin equivalent. It was found to inhibit DPPH and deoxyribose oxidation (IC50 = 24.24 and 5.40 respectively). In vivo study demonstrated a significant reduction in the epithelialization time (P < 0.001) to 17-18 days in normal and dexamethasone treated rats following the ethanolic extract of the bark of C. procera application. The same extract also significantly increased the breaking strength in dexamethasone treated rats. Histological examination of incision wounds of treated group showed matured extracellular matrix, numerous fibroblasts. This study illustrated an excellent potential of the bark of C. procera therapy on dermal wound healing, with a tentative mechanism of action related to improved collagen deposition and reduced inflammatory reaction.

No MeSH data available.


Related in: MedlinePlus

Effect of the ethanol extract of the stem bark of Calotropis procera on wound contraction in rats each value represents the mean ± standard error of mean, n = 5. ***P < 0001: Difference significant when compared to control, DX: dexamethasone, ECP: Ethanol extract of the stem bark of Calotropis procera, ECP+DX: Ethanol extract of the stem bark of Calotropis procera combined with dexamethasone
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Figure 1: Effect of the ethanol extract of the stem bark of Calotropis procera on wound contraction in rats each value represents the mean ± standard error of mean, n = 5. ***P < 0001: Difference significant when compared to control, DX: dexamethasone, ECP: Ethanol extract of the stem bark of Calotropis procera, ECP+DX: Ethanol extract of the stem bark of Calotropis procera combined with dexamethasone

Mentions: Results of the wound contraction rate and epithelialization time are shown in Table 3 and Figure 1 respectively. The extract-treated group demonstrated significantly higher wound contracting ability (P < 0.001) than the control group from day 4 post-wounding. In the extract treated, group complete healing was observed on 17th-18th day, while untreated group (control) and Dexamethasone treated animals took more than 20 days for healing of wounds.


Evaluation of the antioxidant activity and the healing action of the ethanol extract of Calotropis procera bark against surgical wounds.

Tsala DE, Nga N, Thiery BN, Bienvenue MT, Theophile D - J Intercult Ethnopharmacol (2015)

Effect of the ethanol extract of the stem bark of Calotropis procera on wound contraction in rats each value represents the mean ± standard error of mean, n = 5. ***P < 0001: Difference significant when compared to control, DX: dexamethasone, ECP: Ethanol extract of the stem bark of Calotropis procera, ECP+DX: Ethanol extract of the stem bark of Calotropis procera combined with dexamethasone
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4566760&req=5

Figure 1: Effect of the ethanol extract of the stem bark of Calotropis procera on wound contraction in rats each value represents the mean ± standard error of mean, n = 5. ***P < 0001: Difference significant when compared to control, DX: dexamethasone, ECP: Ethanol extract of the stem bark of Calotropis procera, ECP+DX: Ethanol extract of the stem bark of Calotropis procera combined with dexamethasone
Mentions: Results of the wound contraction rate and epithelialization time are shown in Table 3 and Figure 1 respectively. The extract-treated group demonstrated significantly higher wound contracting ability (P < 0.001) than the control group from day 4 post-wounding. In the extract treated, group complete healing was observed on 17th-18th day, while untreated group (control) and Dexamethasone treated animals took more than 20 days for healing of wounds.

Bottom Line: It was found to inhibit DPPH and deoxyribose oxidation (IC50 = 24.24 and 5.40 respectively).In vivo study demonstrated a significant reduction in the epithelialization time (P < 0.001) to 17-18 days in normal and dexamethasone treated rats following the ethanolic extract of the bark of C. procera application.The same extract also significantly increased the breaking strength in dexamethasone treated rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Faculty of Science, University of Maroua, Maroua-Cameroon.

ABSTRACT
The objective of the present study was to evaluate the antioxidant potential and the wound healing effect of the ethanolic extract of the bark of Calotropis procera. The antioxidant study was evaluated in vitro, using 2,2-diphenylpicrylhydrazyl (DPPH) and deoxyribose degradation assays. Wound healing was studied using excision and incision wound on normal and dexamethasone-suppressed wound healing rodent models. Alkaloids, flavonoids, proteins and phenols were screened in the extract used whereas saponins and true tannins were absent. The extract contains only 12.5 gallic acid equivalent and 399.54 rutin equivalent. It was found to inhibit DPPH and deoxyribose oxidation (IC50 = 24.24 and 5.40 respectively). In vivo study demonstrated a significant reduction in the epithelialization time (P < 0.001) to 17-18 days in normal and dexamethasone treated rats following the ethanolic extract of the bark of C. procera application. The same extract also significantly increased the breaking strength in dexamethasone treated rats. Histological examination of incision wounds of treated group showed matured extracellular matrix, numerous fibroblasts. This study illustrated an excellent potential of the bark of C. procera therapy on dermal wound healing, with a tentative mechanism of action related to improved collagen deposition and reduced inflammatory reaction.

No MeSH data available.


Related in: MedlinePlus