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Evaluation of probiotic potential of yeasts isolated from traditional cheeses manufactured in Serbia and Croatia.

Živković M, Čadež N, Uroić K, Miljković M, Tolinački M, Doušova P, Kos B, Šušković J, Raspor P, Topisirović L, Golić N - J Intercult Ethnopharmacol (2015)

Bottom Line: The results revealed that two strains of Kluyvereomyces lactis ZIM 2408 and ZIM 2453 grew above one log unit (Δ log CFU/ml) in the complex colonic medium during 24 h of cultivation, while Torulaspora delbrueckii ZIM 2460 was the most resistant isolate in chemically-simulated conditions of gastric juice and upper intestinal tract.The dairy yeast isolates exhibit strain-specific probiotic properties, particularly the strain K. lactis ZIM 2408, which appears to be the best probiotic candidate in terms of all three criteria.Taking into account their immunomodulatory potential, the yeast isolates could be further tested for specific probiotic applications and eventually included in functional food formulated for patients suffering from diseases associated with an increased inflammatory status.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for Molecular Microbiology, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe, Belgrade, Serbia.

ABSTRACT

Aim: The aim of this study was to investigate the in vitro probiotic potential of dairy yeast isolates from artisanal cheeses manufactured in Serbia and Croatia.

Materials and methods: Twelve yeast strains isolated from artisanal fresh soft and white brined cheeses manufactured in Serbia and Croatia were used in the study. Survival in chemically-simulated gastrointestinal conditions, adherence to epithelial intestinal cells and proliferation of gut-associated lymphoid tissue (GALT) cells were evaluated.

Results: The results revealed that two strains of Kluyvereomyces lactis ZIM 2408 and ZIM 2453 grew above one log unit (Δ log CFU/ml) in the complex colonic medium during 24 h of cultivation, while Torulaspora delbrueckii ZIM 2460 was the most resistant isolate in chemically-simulated conditions of gastric juice and upper intestinal tract. It was demonstrated that the strains K. lactis ZIM 2408 and ZIM2441 and Saccharomyces cerevisiae ZIM 2415 were highly adhesive to Caco-2 cells, while strains K. lactis ZIM 2408 and Debaryomyces hansenii ZIM 2415 exhibit the highest adhesion percentage to HT29-MTX cells. All strains significantly (P < 0.0001) decreased the proliferation of GALT cells, suggesting the possible strain-specific immunomodulatory potential of the isolates.

Conclusion: The dairy yeast isolates exhibit strain-specific probiotic properties, particularly the strain K. lactis ZIM 2408, which appears to be the best probiotic candidate in terms of all three criteria. Taking into account their immunomodulatory potential, the yeast isolates could be further tested for specific probiotic applications and eventually included in functional food formulated for patients suffering from diseases associated with an increased inflammatory status.

No MeSH data available.


Related in: MedlinePlus

Proliferation of GALT cell isolated from rats, co-cultured for four days in the presence of the yeast isolates at ratio (yeast : cell line) 5:1. 1. Kluyveromyces lactis ZIM 2441; 2. K. lactis ZIM 2453; 3. K. lactis ZIM 2408; 4. K. lactis ZIM 2456; 5. Torulaspora delbrueckii ZIM 2458; 6. T. delbrueckii ZIM 2436; 7. T. quercuum ZIM 2412; 8. Torulaspora sp. ZIM 2460; 9. Saccharomyces cerevisiae ZIM 2415; 10. S. cerevisiae ZIM 2440; 11. S. cerevisiae ZIM 2447; 12. Debaryomyces hansenii ZIM 2422; 13. Control (lymphocytes without the stimuli). The Student’s t-test was used for each strain to determine if the data in between the proliferation in the presence of yeasts and for the control to two IEC lines are significantly different from each other, P < 0.001 value was considered significant. Columns that do not share the same letter are statistically different (P < 0.05).
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Figure 3: Proliferation of GALT cell isolated from rats, co-cultured for four days in the presence of the yeast isolates at ratio (yeast : cell line) 5:1. 1. Kluyveromyces lactis ZIM 2441; 2. K. lactis ZIM 2453; 3. K. lactis ZIM 2408; 4. K. lactis ZIM 2456; 5. Torulaspora delbrueckii ZIM 2458; 6. T. delbrueckii ZIM 2436; 7. T. quercuum ZIM 2412; 8. Torulaspora sp. ZIM 2460; 9. Saccharomyces cerevisiae ZIM 2415; 10. S. cerevisiae ZIM 2440; 11. S. cerevisiae ZIM 2447; 12. Debaryomyces hansenii ZIM 2422; 13. Control (lymphocytes without the stimuli). The Student’s t-test was used for each strain to determine if the data in between the proliferation in the presence of yeasts and for the control to two IEC lines are significantly different from each other, P < 0.001 value was considered significant. Columns that do not share the same letter are statistically different (P < 0.05).

Mentions: The proliferation indexes of GALT measured in the presence of the stimuli are presented in Figure 3. In general, the results showed that the proliferation of GALT cells was reduced in the presence of the yeast strains compared to the control (RMPI in the absence of the stimulus). Specifically, the strain K. lactis ZIM 2408 and three Torulaspora isolates (T. delbrueckii ZIM 2436, T. quercuum ZIM 2412 and Torulaspora sp. ZIM 2460) significantly reduced the number of GALT cells in comparison to non-treated GALT cells (P < 0.0001 in all cases, except for S. cerevisiae ZIM 2415 and S. cerevisiae ZIM 2440, P < 0.001). None of the strains increased the proliferation of GALT cells.


Evaluation of probiotic potential of yeasts isolated from traditional cheeses manufactured in Serbia and Croatia.

Živković M, Čadež N, Uroić K, Miljković M, Tolinački M, Doušova P, Kos B, Šušković J, Raspor P, Topisirović L, Golić N - J Intercult Ethnopharmacol (2015)

Proliferation of GALT cell isolated from rats, co-cultured for four days in the presence of the yeast isolates at ratio (yeast : cell line) 5:1. 1. Kluyveromyces lactis ZIM 2441; 2. K. lactis ZIM 2453; 3. K. lactis ZIM 2408; 4. K. lactis ZIM 2456; 5. Torulaspora delbrueckii ZIM 2458; 6. T. delbrueckii ZIM 2436; 7. T. quercuum ZIM 2412; 8. Torulaspora sp. ZIM 2460; 9. Saccharomyces cerevisiae ZIM 2415; 10. S. cerevisiae ZIM 2440; 11. S. cerevisiae ZIM 2447; 12. Debaryomyces hansenii ZIM 2422; 13. Control (lymphocytes without the stimuli). The Student’s t-test was used for each strain to determine if the data in between the proliferation in the presence of yeasts and for the control to two IEC lines are significantly different from each other, P < 0.001 value was considered significant. Columns that do not share the same letter are statistically different (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4566759&req=5

Figure 3: Proliferation of GALT cell isolated from rats, co-cultured for four days in the presence of the yeast isolates at ratio (yeast : cell line) 5:1. 1. Kluyveromyces lactis ZIM 2441; 2. K. lactis ZIM 2453; 3. K. lactis ZIM 2408; 4. K. lactis ZIM 2456; 5. Torulaspora delbrueckii ZIM 2458; 6. T. delbrueckii ZIM 2436; 7. T. quercuum ZIM 2412; 8. Torulaspora sp. ZIM 2460; 9. Saccharomyces cerevisiae ZIM 2415; 10. S. cerevisiae ZIM 2440; 11. S. cerevisiae ZIM 2447; 12. Debaryomyces hansenii ZIM 2422; 13. Control (lymphocytes without the stimuli). The Student’s t-test was used for each strain to determine if the data in between the proliferation in the presence of yeasts and for the control to two IEC lines are significantly different from each other, P < 0.001 value was considered significant. Columns that do not share the same letter are statistically different (P < 0.05).
Mentions: The proliferation indexes of GALT measured in the presence of the stimuli are presented in Figure 3. In general, the results showed that the proliferation of GALT cells was reduced in the presence of the yeast strains compared to the control (RMPI in the absence of the stimulus). Specifically, the strain K. lactis ZIM 2408 and three Torulaspora isolates (T. delbrueckii ZIM 2436, T. quercuum ZIM 2412 and Torulaspora sp. ZIM 2460) significantly reduced the number of GALT cells in comparison to non-treated GALT cells (P < 0.0001 in all cases, except for S. cerevisiae ZIM 2415 and S. cerevisiae ZIM 2440, P < 0.001). None of the strains increased the proliferation of GALT cells.

Bottom Line: The results revealed that two strains of Kluyvereomyces lactis ZIM 2408 and ZIM 2453 grew above one log unit (Δ log CFU/ml) in the complex colonic medium during 24 h of cultivation, while Torulaspora delbrueckii ZIM 2460 was the most resistant isolate in chemically-simulated conditions of gastric juice and upper intestinal tract.The dairy yeast isolates exhibit strain-specific probiotic properties, particularly the strain K. lactis ZIM 2408, which appears to be the best probiotic candidate in terms of all three criteria.Taking into account their immunomodulatory potential, the yeast isolates could be further tested for specific probiotic applications and eventually included in functional food formulated for patients suffering from diseases associated with an increased inflammatory status.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for Molecular Microbiology, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe, Belgrade, Serbia.

ABSTRACT

Aim: The aim of this study was to investigate the in vitro probiotic potential of dairy yeast isolates from artisanal cheeses manufactured in Serbia and Croatia.

Materials and methods: Twelve yeast strains isolated from artisanal fresh soft and white brined cheeses manufactured in Serbia and Croatia were used in the study. Survival in chemically-simulated gastrointestinal conditions, adherence to epithelial intestinal cells and proliferation of gut-associated lymphoid tissue (GALT) cells were evaluated.

Results: The results revealed that two strains of Kluyvereomyces lactis ZIM 2408 and ZIM 2453 grew above one log unit (Δ log CFU/ml) in the complex colonic medium during 24 h of cultivation, while Torulaspora delbrueckii ZIM 2460 was the most resistant isolate in chemically-simulated conditions of gastric juice and upper intestinal tract. It was demonstrated that the strains K. lactis ZIM 2408 and ZIM2441 and Saccharomyces cerevisiae ZIM 2415 were highly adhesive to Caco-2 cells, while strains K. lactis ZIM 2408 and Debaryomyces hansenii ZIM 2415 exhibit the highest adhesion percentage to HT29-MTX cells. All strains significantly (P < 0.0001) decreased the proliferation of GALT cells, suggesting the possible strain-specific immunomodulatory potential of the isolates.

Conclusion: The dairy yeast isolates exhibit strain-specific probiotic properties, particularly the strain K. lactis ZIM 2408, which appears to be the best probiotic candidate in terms of all three criteria. Taking into account their immunomodulatory potential, the yeast isolates could be further tested for specific probiotic applications and eventually included in functional food formulated for patients suffering from diseases associated with an increased inflammatory status.

No MeSH data available.


Related in: MedlinePlus