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Galectin-1 regulates tissue exit of specific dendritic cell populations.

Thiemann S, Man JH, Chang MH, Lee B, Baum LG - J. Biol. Chem. (2015)

Bottom Line: The presence of galectin-1 inhibits migration of immunogenic dendritic cells through the extracellular matrix and across lymphatic endothelial cells, but it has no effect on migration of tolerogenic dendritic cells.The major galectin-1 counter-receptor on both dendritic cell populations is the cell surface mucin CD43; differential core 2 O-glycosylation of CD43 between immunogenic dendritic cells and tolerogenic dendritic cells appears to contribute to the differential effect of galectin-1 on migration.Binding of galectin-1 to immunogenic dendritic cells reduces phosphorylation and activity of the protein-tyrosine kinase Pyk2, an effect that may also contribute to reduced migration of this subset.

View Article: PubMed Central - PubMed

Affiliation: From the Departments of Pathology and Laboratory Medicine and.

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Human lymphatic endothelial cells express and secrete galectin-1. Sections of skin from lymphedema patients were stained with polyclonal antibody against galectin-1 (top) or monoclonal antibody to the human LEC marker podoplanin (bottom). Bound antibody was detected with the corresponding secondary antibody and visualized using a 3-amino-9-ethylcarbazole chromogenic substrate system. Sections were counterstained with hematoxylin. Insets (middle column) show control antibody staining. Dilated lymphatic vessels are lined by LECs expressing galectin-1 (arrow, top) and podoplanin (arrow, bottom). Data are representative of six independent tissue samples. Note that the distribution of galectin-1 on LECs appears more dispersed than that of podoplanin, suggesting the localization of secreted galectin-1 in extracellular matrix (arrowhead, top right panel). Magnification is as follows: ×20 (left), ×40 (middle), and ×100 (right). Scale bar, 100 μm (left), 50 μm (middle), and 20 μm (right).
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Figure 1: Human lymphatic endothelial cells express and secrete galectin-1. Sections of skin from lymphedema patients were stained with polyclonal antibody against galectin-1 (top) or monoclonal antibody to the human LEC marker podoplanin (bottom). Bound antibody was detected with the corresponding secondary antibody and visualized using a 3-amino-9-ethylcarbazole chromogenic substrate system. Sections were counterstained with hematoxylin. Insets (middle column) show control antibody staining. Dilated lymphatic vessels are lined by LECs expressing galectin-1 (arrow, top) and podoplanin (arrow, bottom). Data are representative of six independent tissue samples. Note that the distribution of galectin-1 on LECs appears more dispersed than that of podoplanin, suggesting the localization of secreted galectin-1 in extracellular matrix (arrowhead, top right panel). Magnification is as follows: ×20 (left), ×40 (middle), and ×100 (right). Scale bar, 100 μm (left), 50 μm (middle), and 20 μm (right).

Mentions: Galectin-1 is expressed by human VECs, and VEC expression of galectin-1 is increased by inflammatory stimuli (38, 39). However, galectin-1 expression by LECs in inflamed human tissue in vivo has not been described. To characterize galectin-1 expression by LECs in inflamed tissue, we examined sections of lymphedematous human skin (Fig. 1). Sections were stained with pAb against human galectin-1 (Fig. 1, top row) or a mAb recognizing the LEC marker podoplanin (Fig. 1, bottom row) (60). In the dermis, galectin-1 was detected in endothelial cells (arrow, top row) lining dilated vessels; these same cells also expressed podoplanin (arrow, bottom row), confirming that the cells were LECs. In addition, there was abundant galectin-1 in the extracellular matrix surrounding lymphatic channels (arrowhead, top right panel). We also detected galectin-1 in infiltrating leukocytes in the dermis.


Galectin-1 regulates tissue exit of specific dendritic cell populations.

Thiemann S, Man JH, Chang MH, Lee B, Baum LG - J. Biol. Chem. (2015)

Human lymphatic endothelial cells express and secrete galectin-1. Sections of skin from lymphedema patients were stained with polyclonal antibody against galectin-1 (top) or monoclonal antibody to the human LEC marker podoplanin (bottom). Bound antibody was detected with the corresponding secondary antibody and visualized using a 3-amino-9-ethylcarbazole chromogenic substrate system. Sections were counterstained with hematoxylin. Insets (middle column) show control antibody staining. Dilated lymphatic vessels are lined by LECs expressing galectin-1 (arrow, top) and podoplanin (arrow, bottom). Data are representative of six independent tissue samples. Note that the distribution of galectin-1 on LECs appears more dispersed than that of podoplanin, suggesting the localization of secreted galectin-1 in extracellular matrix (arrowhead, top right panel). Magnification is as follows: ×20 (left), ×40 (middle), and ×100 (right). Scale bar, 100 μm (left), 50 μm (middle), and 20 μm (right).
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4566239&req=5

Figure 1: Human lymphatic endothelial cells express and secrete galectin-1. Sections of skin from lymphedema patients were stained with polyclonal antibody against galectin-1 (top) or monoclonal antibody to the human LEC marker podoplanin (bottom). Bound antibody was detected with the corresponding secondary antibody and visualized using a 3-amino-9-ethylcarbazole chromogenic substrate system. Sections were counterstained with hematoxylin. Insets (middle column) show control antibody staining. Dilated lymphatic vessels are lined by LECs expressing galectin-1 (arrow, top) and podoplanin (arrow, bottom). Data are representative of six independent tissue samples. Note that the distribution of galectin-1 on LECs appears more dispersed than that of podoplanin, suggesting the localization of secreted galectin-1 in extracellular matrix (arrowhead, top right panel). Magnification is as follows: ×20 (left), ×40 (middle), and ×100 (right). Scale bar, 100 μm (left), 50 μm (middle), and 20 μm (right).
Mentions: Galectin-1 is expressed by human VECs, and VEC expression of galectin-1 is increased by inflammatory stimuli (38, 39). However, galectin-1 expression by LECs in inflamed human tissue in vivo has not been described. To characterize galectin-1 expression by LECs in inflamed tissue, we examined sections of lymphedematous human skin (Fig. 1). Sections were stained with pAb against human galectin-1 (Fig. 1, top row) or a mAb recognizing the LEC marker podoplanin (Fig. 1, bottom row) (60). In the dermis, galectin-1 was detected in endothelial cells (arrow, top row) lining dilated vessels; these same cells also expressed podoplanin (arrow, bottom row), confirming that the cells were LECs. In addition, there was abundant galectin-1 in the extracellular matrix surrounding lymphatic channels (arrowhead, top right panel). We also detected galectin-1 in infiltrating leukocytes in the dermis.

Bottom Line: The presence of galectin-1 inhibits migration of immunogenic dendritic cells through the extracellular matrix and across lymphatic endothelial cells, but it has no effect on migration of tolerogenic dendritic cells.The major galectin-1 counter-receptor on both dendritic cell populations is the cell surface mucin CD43; differential core 2 O-glycosylation of CD43 between immunogenic dendritic cells and tolerogenic dendritic cells appears to contribute to the differential effect of galectin-1 on migration.Binding of galectin-1 to immunogenic dendritic cells reduces phosphorylation and activity of the protein-tyrosine kinase Pyk2, an effect that may also contribute to reduced migration of this subset.

View Article: PubMed Central - PubMed

Affiliation: From the Departments of Pathology and Laboratory Medicine and.

Show MeSH
Related in: MedlinePlus