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Companion cropping with potato onion enhances the disease resistance of tomato against Verticillium dahliae.

Fu X, Wu X, Zhou X, Liu S, Shen Y, Wu F - Front Plant Sci (2015)

Bottom Line: The results showed that companion cropping with potato onion could alleviate the incidence and severity of tomato Verticillium wilt.The further studies revealed that the root exudates from tomato companied with potato onion significantly inhibited the mycelia growth and spore germination of V. dahliae.However, there were no significant effects on these two measurements for the root exudates from potato onion grown alone or from potato onion grown with tomato.

View Article: PubMed Central - PubMed

Affiliation: Department of Horticulture, Northeast Agricultural University Harbin, China ; Department of Life Science and Agroforestry, Qiqihar University Qiqihar, China.

ABSTRACT
Intercropping could alleviate soil-borne diseases, however, few studies focused on the immunity of the host plant induced by the interspecific interactions. To test whether or not intercropping could enhance the disease resistance of host plant, we investigated the effect of companion cropping with potato onion on tomato Verticillium wilt caused by Verticillium dahliae (V. dahliae). To investigate the mechanisms, the root exudates were collected from tomato and potato onion which were grown together or separately, and were used to examine the antifungal activities against V. dahliae in vitro, respectively. Furthermore, RNA-seq was used to examine the expression pattern of genes related to disease resistance in tomato companied with potato onion compared to that in tomato grown alone, under the condition of infection with V. dahliae. The results showed that companion cropping with potato onion could alleviate the incidence and severity of tomato Verticillium wilt. The further studies revealed that the root exudates from tomato companied with potato onion significantly inhibited the mycelia growth and spore germination of V. dahliae. However, there were no significant effects on these two measurements for the root exudates from potato onion grown alone or from potato onion grown with tomato. RNA-seq data analysis showed the disease defense genes associated with pathogenesis-related proteins, biosynthesis of lignin, hormone metabolism and signal transduction were expressed much higher in the tomato companied with potato onion than those in the tomato grown alone, which indicated that these defense genes play important roles in tomato against V. dahliae infection, and meant that the disease resistance of tomato against V. dahliae was enhanced in the companion copping with potato onion. We proposed that companion cropping with potato onion could enhance the disease resistance of tomato against V. dahliae by regulating the expression of genes related to disease resistance response. This may be a potential mechanism for the management of soil-borne plant diseases in the intercropping system.

No MeSH data available.


Related in: MedlinePlus

Disease incidence and disease symptoms score of tomato inoculated with Vd1. (A,B) Disease incidence of tomato in 2013 and 2014, respectively. (C,D) Disease symptoms score in 2013 and 2014, respectively. 18 DAI and 28 DAT indicate 18 and 28 days after infected by Vd1, respectively. Data were the means of three replicates with standards errors shown by vertical bars. The different small letters above the bars represent the significance between two groups of mean values at a lever of p = 0.05 according to independent sample t-test.
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Figure 1: Disease incidence and disease symptoms score of tomato inoculated with Vd1. (A,B) Disease incidence of tomato in 2013 and 2014, respectively. (C,D) Disease symptoms score in 2013 and 2014, respectively. 18 DAI and 28 DAT indicate 18 and 28 days after infected by Vd1, respectively. Data were the means of three replicates with standards errors shown by vertical bars. The different small letters above the bars represent the significance between two groups of mean values at a lever of p = 0.05 according to independent sample t-test.

Mentions: The results regarding to the disease incidence and disease symptoms score of tomato were shown in Figure 1. Compared to TM (tomato monoculture), the disease incidences in TC (tomato companied with potato onion) were decreased by 24.97 and 27.13% in 2013, by 35.58 and 19.83% in 2014 at 18 and 28 DAI, respectively (Figures 1A,B). Similarly, the disease symptoms scores of tomato were significantly declined (p ≤ 0.05) in TC compared to TM at all observed stages except 18 DAI in 2013, in which disease symptoms scores was also lower, but not significant (Figures 1C,D).


Companion cropping with potato onion enhances the disease resistance of tomato against Verticillium dahliae.

Fu X, Wu X, Zhou X, Liu S, Shen Y, Wu F - Front Plant Sci (2015)

Disease incidence and disease symptoms score of tomato inoculated with Vd1. (A,B) Disease incidence of tomato in 2013 and 2014, respectively. (C,D) Disease symptoms score in 2013 and 2014, respectively. 18 DAI and 28 DAT indicate 18 and 28 days after infected by Vd1, respectively. Data were the means of three replicates with standards errors shown by vertical bars. The different small letters above the bars represent the significance between two groups of mean values at a lever of p = 0.05 according to independent sample t-test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4566073&req=5

Figure 1: Disease incidence and disease symptoms score of tomato inoculated with Vd1. (A,B) Disease incidence of tomato in 2013 and 2014, respectively. (C,D) Disease symptoms score in 2013 and 2014, respectively. 18 DAI and 28 DAT indicate 18 and 28 days after infected by Vd1, respectively. Data were the means of three replicates with standards errors shown by vertical bars. The different small letters above the bars represent the significance between two groups of mean values at a lever of p = 0.05 according to independent sample t-test.
Mentions: The results regarding to the disease incidence and disease symptoms score of tomato were shown in Figure 1. Compared to TM (tomato monoculture), the disease incidences in TC (tomato companied with potato onion) were decreased by 24.97 and 27.13% in 2013, by 35.58 and 19.83% in 2014 at 18 and 28 DAI, respectively (Figures 1A,B). Similarly, the disease symptoms scores of tomato were significantly declined (p ≤ 0.05) in TC compared to TM at all observed stages except 18 DAI in 2013, in which disease symptoms scores was also lower, but not significant (Figures 1C,D).

Bottom Line: The results showed that companion cropping with potato onion could alleviate the incidence and severity of tomato Verticillium wilt.The further studies revealed that the root exudates from tomato companied with potato onion significantly inhibited the mycelia growth and spore germination of V. dahliae.However, there were no significant effects on these two measurements for the root exudates from potato onion grown alone or from potato onion grown with tomato.

View Article: PubMed Central - PubMed

Affiliation: Department of Horticulture, Northeast Agricultural University Harbin, China ; Department of Life Science and Agroforestry, Qiqihar University Qiqihar, China.

ABSTRACT
Intercropping could alleviate soil-borne diseases, however, few studies focused on the immunity of the host plant induced by the interspecific interactions. To test whether or not intercropping could enhance the disease resistance of host plant, we investigated the effect of companion cropping with potato onion on tomato Verticillium wilt caused by Verticillium dahliae (V. dahliae). To investigate the mechanisms, the root exudates were collected from tomato and potato onion which were grown together or separately, and were used to examine the antifungal activities against V. dahliae in vitro, respectively. Furthermore, RNA-seq was used to examine the expression pattern of genes related to disease resistance in tomato companied with potato onion compared to that in tomato grown alone, under the condition of infection with V. dahliae. The results showed that companion cropping with potato onion could alleviate the incidence and severity of tomato Verticillium wilt. The further studies revealed that the root exudates from tomato companied with potato onion significantly inhibited the mycelia growth and spore germination of V. dahliae. However, there were no significant effects on these two measurements for the root exudates from potato onion grown alone or from potato onion grown with tomato. RNA-seq data analysis showed the disease defense genes associated with pathogenesis-related proteins, biosynthesis of lignin, hormone metabolism and signal transduction were expressed much higher in the tomato companied with potato onion than those in the tomato grown alone, which indicated that these defense genes play important roles in tomato against V. dahliae infection, and meant that the disease resistance of tomato against V. dahliae was enhanced in the companion copping with potato onion. We proposed that companion cropping with potato onion could enhance the disease resistance of tomato against V. dahliae by regulating the expression of genes related to disease resistance response. This may be a potential mechanism for the management of soil-borne plant diseases in the intercropping system.

No MeSH data available.


Related in: MedlinePlus