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Induction of ROS generation and NF-κB activation in MARC-145 cells by a novel porcine reproductive and respiratory syndrome virus in Southwest of China isolate.

Yan Y, Xin A, Liu Q, Huang H, Shao Z, Zang Y, Chen L, Sun Y, Gao H - BMC Vet. Res. (2015)

Bottom Line: In PRRSV infected MARC-145 cells, there was a time-dependent increase in ROS and Maleic Dialdehyde (MDA).The results indicate that the generation of ROS is involved in PRRSV replication and this progression is associated with the alteration in NF-κB activity induced by ROS.These results should extend our better understanding the interaction between PRRSV and host MARC-145 cells.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, People's Republic of China. yanyulin333@163.com.

ABSTRACT

Background: Porcine reproductive and respiratory syndrome virus (PRRSV) is the cause of an economically important swine disease that has devastated the swine industry since the late 1980s. The aim of the present study was to investigate the interaction between reactive oxygen species (ROS) and NF-κB by PRRSV infection.

Results: We isolated the local strain of PRRSV from southwest China, designated YN-2011, then sequenced and analyzed the genome. YN-2011 was then used to evaluate the interaction of ROS and NF-κB. In PRRSV infected MARC-145 cells, there was a time-dependent increase in ROS and Maleic Dialdehyde (MDA). Accordingly, NF-κB activation was also increased as PRRSV infection progressed. Degradation of IκB mRNA was detected late in PRRSV infection, and overexpression of the dominant negative form of IκBα significantly suppressed NF-κB induced by PRRSV.

Conclusions: The results indicate that the generation of ROS is involved in PRRSV replication and this progression is associated with the alteration in NF-κB activity induced by ROS. These results should extend our better understanding the interaction between PRRSV and host MARC-145 cells.

No MeSH data available.


Related in: MedlinePlus

IκBα mRNA levels in MARC-145 cells infected with PRRSV. Cultures were infected with PRRSV at MOI = 1. Cells were collected at the indicated time points, and the endogenous transcription of IκBα mRNA was analyzed by real-time RT-PCR. The level of IκBα was first normalized to that of β-actin in the same sample and then compared with the control cells. *P < 0.05, **P < 0.01
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Fig7: IκBα mRNA levels in MARC-145 cells infected with PRRSV. Cultures were infected with PRRSV at MOI = 1. Cells were collected at the indicated time points, and the endogenous transcription of IκBα mRNA was analyzed by real-time RT-PCR. The level of IκBα was first normalized to that of β-actin in the same sample and then compared with the control cells. *P < 0.05, **P < 0.01

Mentions: Activation of NF-κB is characterized by degradation of IκBα after phosphorylation by IKK in response to many types of extracellular stimuli [39, 52]. This is followed by the phosphorylation of the NF-κB subunit p65 and nuclear translocation of NF-κB. To investigate the different time and potential mechanism(s) of NF-κB activation by PRRSV, IκBα gene expression levels were examined using quantitative real-time RT-PCR. As demonstrated in Fig. 7, IκBa was detected in both PRRSV infected and uninfected MARC-145 cells at 0, and 12, and 24 hpi. However, there was a significant decrease in IκBa mRNA levels at 36, 48, and 60 hpi in PRRSV infected MARC-145 cells compared to uninfected controls. The lowest level of IκBa mRNA was detected at 48 hpi, which correlated with the highest level of NF-κB activity.Fig. 7


Induction of ROS generation and NF-κB activation in MARC-145 cells by a novel porcine reproductive and respiratory syndrome virus in Southwest of China isolate.

Yan Y, Xin A, Liu Q, Huang H, Shao Z, Zang Y, Chen L, Sun Y, Gao H - BMC Vet. Res. (2015)

IκBα mRNA levels in MARC-145 cells infected with PRRSV. Cultures were infected with PRRSV at MOI = 1. Cells were collected at the indicated time points, and the endogenous transcription of IκBα mRNA was analyzed by real-time RT-PCR. The level of IκBα was first normalized to that of β-actin in the same sample and then compared with the control cells. *P < 0.05, **P < 0.01
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4565009&req=5

Fig7: IκBα mRNA levels in MARC-145 cells infected with PRRSV. Cultures were infected with PRRSV at MOI = 1. Cells were collected at the indicated time points, and the endogenous transcription of IκBα mRNA was analyzed by real-time RT-PCR. The level of IκBα was first normalized to that of β-actin in the same sample and then compared with the control cells. *P < 0.05, **P < 0.01
Mentions: Activation of NF-κB is characterized by degradation of IκBα after phosphorylation by IKK in response to many types of extracellular stimuli [39, 52]. This is followed by the phosphorylation of the NF-κB subunit p65 and nuclear translocation of NF-κB. To investigate the different time and potential mechanism(s) of NF-κB activation by PRRSV, IκBα gene expression levels were examined using quantitative real-time RT-PCR. As demonstrated in Fig. 7, IκBa was detected in both PRRSV infected and uninfected MARC-145 cells at 0, and 12, and 24 hpi. However, there was a significant decrease in IκBa mRNA levels at 36, 48, and 60 hpi in PRRSV infected MARC-145 cells compared to uninfected controls. The lowest level of IκBa mRNA was detected at 48 hpi, which correlated with the highest level of NF-κB activity.Fig. 7

Bottom Line: In PRRSV infected MARC-145 cells, there was a time-dependent increase in ROS and Maleic Dialdehyde (MDA).The results indicate that the generation of ROS is involved in PRRSV replication and this progression is associated with the alteration in NF-κB activity induced by ROS.These results should extend our better understanding the interaction between PRRSV and host MARC-145 cells.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, People's Republic of China. yanyulin333@163.com.

ABSTRACT

Background: Porcine reproductive and respiratory syndrome virus (PRRSV) is the cause of an economically important swine disease that has devastated the swine industry since the late 1980s. The aim of the present study was to investigate the interaction between reactive oxygen species (ROS) and NF-κB by PRRSV infection.

Results: We isolated the local strain of PRRSV from southwest China, designated YN-2011, then sequenced and analyzed the genome. YN-2011 was then used to evaluate the interaction of ROS and NF-κB. In PRRSV infected MARC-145 cells, there was a time-dependent increase in ROS and Maleic Dialdehyde (MDA). Accordingly, NF-κB activation was also increased as PRRSV infection progressed. Degradation of IκB mRNA was detected late in PRRSV infection, and overexpression of the dominant negative form of IκBα significantly suppressed NF-κB induced by PRRSV.

Conclusions: The results indicate that the generation of ROS is involved in PRRSV replication and this progression is associated with the alteration in NF-κB activity induced by ROS. These results should extend our better understanding the interaction between PRRSV and host MARC-145 cells.

No MeSH data available.


Related in: MedlinePlus