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Silk Gland Gene Expression during Larval-Pupal Transition in the Cotton Leaf Roller Sylepta derogata (Lepidoptera: Pyralidae).

Su H, Cheng Y, Wang Z, Li Z, Stanley D, Yang Y - PLoS ONE (2015)

Bottom Line: We tested our hypothesis using RNA-seq to investigate changes in silk gland gene expression at three developmental stages, 5th instar larvae (silk producing; 15,445,926 clean reads), prepupae (reduced silk producing; 13,758,154) and pupae (beyond silk producing; 16,787,792).We recorded highest expression of this gene in the larval silk gland, compared to other tissues, including midgut, hindgut, epidermis, Malpighian tubes, hemolymph and fat body.These data are a genetic resource to guide selection of key genes that may be targeted for in planta and other gene-silencing technologies for sustainable cotton agriculture.

View Article: PubMed Central - PubMed

Affiliation: School of Horticulture and Plant Protection of Yangzhou University, Yangzhou, Jiangsu, China, 225009.

ABSTRACT
The cotton leaf roller, Sylepta derogata, is a silk-producing insect pest. While young larvae feed on the underside of leaves, the older ones roll cotton leaves and feed on the leaf edges, which defoliates cotton plants. The larvae produce silk to stabilize the rolled leaf and to balloon from used to new leaves. Despite the significance of silk in the biology of pest insect species, there is virtually no information on the genes involved in their silk production. This is a substantial knowledge gap because some of these genes may be valuable targets for developing molecular pest management technologies. We addressed the gap by posing the hypothesis that silk gland gene expression changes during the transition from larvae to pupae. We tested our hypothesis using RNA-seq to investigate changes in silk gland gene expression at three developmental stages, 5th instar larvae (silk producing; 15,445,926 clean reads), prepupae (reduced silk producing; 13,758,154) and pupae (beyond silk producing; 16,787,792). We recorded 60,298 unigenes and mapped 50,158 (larvae), 48,415 (prepupae) and 46,623 (pupae) of them to the NCBI database. Most differentially expressed genes in the 5th instar larvae/prepupae libraries were relevant to nucleotide synthesis and maintenance of silk gland function. We identified down-regulated transcriptional factors and several genes involved in silk formation in the three libraries and verified the expression pattern of eight genes by qPCR. The developmental- and tissue-specific expression patterns of the fibroin light chain gene showed it was highly expressed during the larval silk-producing stage. We recorded highest expression of this gene in the larval silk gland, compared to other tissues, including midgut, hindgut, epidermis, Malpighian tubes, hemolymph and fat body. These data are a genetic resource to guide selection of key genes that may be targeted for in planta and other gene-silencing technologies for sustainable cotton agriculture.

No MeSH data available.


Related in: MedlinePlus

A pie chart showing proportions (with numbers in parentheses) of transcripts annotated by NR, Swiss-prot, NT and KEGG databases.
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pone.0136868.g001: A pie chart showing proportions (with numbers in parentheses) of transcripts annotated by NR, Swiss-prot, NT and KEGG databases.

Mentions: All distinct sequences longer than 200 bp were searched against the NR, Swiss-prot, and KEGG protein databases by BLASTX with a cut-off E-value of 1e-10. We recorded 19,090 (41.1% of all distinct sequences; by NR), 12,538 (27.0%; Swiss-prot), 11,260 (24.3%; NT) and 1,567 (3.4%; KEGG) transcripts (Fig 1). Identities of many genes (about 37%) were not found in available databases, as expected due to the lack of transcriptomic information in tissues within pest species.


Silk Gland Gene Expression during Larval-Pupal Transition in the Cotton Leaf Roller Sylepta derogata (Lepidoptera: Pyralidae).

Su H, Cheng Y, Wang Z, Li Z, Stanley D, Yang Y - PLoS ONE (2015)

A pie chart showing proportions (with numbers in parentheses) of transcripts annotated by NR, Swiss-prot, NT and KEGG databases.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4564283&req=5

pone.0136868.g001: A pie chart showing proportions (with numbers in parentheses) of transcripts annotated by NR, Swiss-prot, NT and KEGG databases.
Mentions: All distinct sequences longer than 200 bp were searched against the NR, Swiss-prot, and KEGG protein databases by BLASTX with a cut-off E-value of 1e-10. We recorded 19,090 (41.1% of all distinct sequences; by NR), 12,538 (27.0%; Swiss-prot), 11,260 (24.3%; NT) and 1,567 (3.4%; KEGG) transcripts (Fig 1). Identities of many genes (about 37%) were not found in available databases, as expected due to the lack of transcriptomic information in tissues within pest species.

Bottom Line: We tested our hypothesis using RNA-seq to investigate changes in silk gland gene expression at three developmental stages, 5th instar larvae (silk producing; 15,445,926 clean reads), prepupae (reduced silk producing; 13,758,154) and pupae (beyond silk producing; 16,787,792).We recorded highest expression of this gene in the larval silk gland, compared to other tissues, including midgut, hindgut, epidermis, Malpighian tubes, hemolymph and fat body.These data are a genetic resource to guide selection of key genes that may be targeted for in planta and other gene-silencing technologies for sustainable cotton agriculture.

View Article: PubMed Central - PubMed

Affiliation: School of Horticulture and Plant Protection of Yangzhou University, Yangzhou, Jiangsu, China, 225009.

ABSTRACT
The cotton leaf roller, Sylepta derogata, is a silk-producing insect pest. While young larvae feed on the underside of leaves, the older ones roll cotton leaves and feed on the leaf edges, which defoliates cotton plants. The larvae produce silk to stabilize the rolled leaf and to balloon from used to new leaves. Despite the significance of silk in the biology of pest insect species, there is virtually no information on the genes involved in their silk production. This is a substantial knowledge gap because some of these genes may be valuable targets for developing molecular pest management technologies. We addressed the gap by posing the hypothesis that silk gland gene expression changes during the transition from larvae to pupae. We tested our hypothesis using RNA-seq to investigate changes in silk gland gene expression at three developmental stages, 5th instar larvae (silk producing; 15,445,926 clean reads), prepupae (reduced silk producing; 13,758,154) and pupae (beyond silk producing; 16,787,792). We recorded 60,298 unigenes and mapped 50,158 (larvae), 48,415 (prepupae) and 46,623 (pupae) of them to the NCBI database. Most differentially expressed genes in the 5th instar larvae/prepupae libraries were relevant to nucleotide synthesis and maintenance of silk gland function. We identified down-regulated transcriptional factors and several genes involved in silk formation in the three libraries and verified the expression pattern of eight genes by qPCR. The developmental- and tissue-specific expression patterns of the fibroin light chain gene showed it was highly expressed during the larval silk-producing stage. We recorded highest expression of this gene in the larval silk gland, compared to other tissues, including midgut, hindgut, epidermis, Malpighian tubes, hemolymph and fat body. These data are a genetic resource to guide selection of key genes that may be targeted for in planta and other gene-silencing technologies for sustainable cotton agriculture.

No MeSH data available.


Related in: MedlinePlus