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Dietary Fish Oil Inhibits Pro-Inflammatory and ER Stress Signalling Pathways in the Liver of Sows during Lactation.

Gessner DK, Gröne B, Couturier A, Rosenbaum S, Hillen S, Becker S, Erhardt G, Reiner G, Ringseis R, Eder K - PLoS ONE (2015)

Bottom Line: This was demonstrated by decreased mRNA levels of a number of UPR-regulated genes and reduced phosphorylation of PERK and PERK-mediated phosphorylation of eIF2α and IκB in the liver of the fish oil group.In line with this, the plasma levels of acute phase proteins were reduced in the fish oil group, although differences to the control group were not significant.In conclusion, ER stress-induced UPR is present in the liver of lactating sows and fish oil is able to inhibit inflammatory signaling pathways and ER stress-induced UPR in the liver.

View Article: PubMed Central - PubMed

Affiliation: Institute of Animal Nutrition and Nutrition Physiology, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany.

ABSTRACT
Lactating sows have been shown to develop typical signs of an inflammatory condition in the liver during the transition from pregnancy to lactation. Hepatic inflammation is considered critical due to the induction of an acute phase response and the activation of stress signaling pathways like the endoplasmic reticulum (ER) stress-induced unfolded protein response (UPR), both of which impair animal's health and performance. Whether ER stress-induced UPR is also activated in the liver of lactating sows and whether dietary fish oil as a source of anti-inflammatory effects n-3 PUFA is able to attenuate hepatic inflammation and ER stress-induced UPR in the liver of sows is currently unknown. Based on this, two experiments with lactating sows were performed. The first experiment revealed that ER stress-induced UPR occurs also in the liver of sows during lactation. This was evident from the up-regulation of a set of genes regulated by the UPR and numerically increased phosphorylation of the ER stress-transducer PERK and PERK-mediated phosphorylation of eIF2α and IκB. The second experiment showed that fish oil inhibits ER stress-induced UPR in the liver of lactating sows. This was demonstrated by decreased mRNA levels of a number of UPR-regulated genes and reduced phosphorylation of PERK and PERK-mediated phosphorylation of eIF2α and IκB in the liver of the fish oil group. The mRNA levels of various nuclear factor-κB-regulated genes encoding inflammatory mediators and acute phase proteins in the liver of lactating sows were also reduced in the fish oil group. In line with this, the plasma levels of acute phase proteins were reduced in the fish oil group, although differences to the control group were not significant. In conclusion, ER stress-induced UPR is present in the liver of lactating sows and fish oil is able to inhibit inflammatory signaling pathways and ER stress-induced UPR in the liver.

No MeSH data available.


Related in: MedlinePlus

Effect of fish oil on mRNA concentrations of genes involved in the NLRP3 inflammasome in the liver and skeletal muscle of lactating sows.Relative mRNA concentrations genes involved in the NOD-like receptor P3 (NLRP3) inflammasome in the liver (A) and the skeletal muscle (B) of lactating sows with or without fish oil supplementation on day 20 of lactation. Filled circles (grey = control group, black = fish oil group) represent individual data for each animal. Black lines represent means of individual data for each group (n = 6–10 sows per group). The mean of the control group is set to 1. The mean of the fish oil group is expressed as fold of the control group and numerically indicated next to the black line. Superscript symbol indicates difference from control group (*p<0.05). Abbreviations: CASP1, caspase 1, apoptosis-related cysteine peptidase; IL1B, interleukin 1, beta; NLRP3, NLR family, pyrin domain containing 3; PYCARD, PYD and CARD domain containing.
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pone.0137684.g009: Effect of fish oil on mRNA concentrations of genes involved in the NLRP3 inflammasome in the liver and skeletal muscle of lactating sows.Relative mRNA concentrations genes involved in the NOD-like receptor P3 (NLRP3) inflammasome in the liver (A) and the skeletal muscle (B) of lactating sows with or without fish oil supplementation on day 20 of lactation. Filled circles (grey = control group, black = fish oil group) represent individual data for each animal. Black lines represent means of individual data for each group (n = 6–10 sows per group). The mean of the control group is set to 1. The mean of the fish oil group is expressed as fold of the control group and numerically indicated next to the black line. Superscript symbol indicates difference from control group (*p<0.05). Abbreviations: CASP1, caspase 1, apoptosis-related cysteine peptidase; IL1B, interleukin 1, beta; NLRP3, NLR family, pyrin domain containing 3; PYCARD, PYD and CARD domain containing.

Mentions: The relative mRNA concentrations of four genes involved in the NLRP3 inflammasome (CASP1, NLRP3, PYCARD, IL1B) in the liver did not differ between the fish oil group and the control group (Fig 9A). In skeletal muscle the relative mRNA concentration of PYCARD was reduced in the fish oil group compared to the control group (p<0.05; Fig 9B), whereas the other genes (CASP1, NLRP3, IL1B) were not different between groups.


Dietary Fish Oil Inhibits Pro-Inflammatory and ER Stress Signalling Pathways in the Liver of Sows during Lactation.

Gessner DK, Gröne B, Couturier A, Rosenbaum S, Hillen S, Becker S, Erhardt G, Reiner G, Ringseis R, Eder K - PLoS ONE (2015)

Effect of fish oil on mRNA concentrations of genes involved in the NLRP3 inflammasome in the liver and skeletal muscle of lactating sows.Relative mRNA concentrations genes involved in the NOD-like receptor P3 (NLRP3) inflammasome in the liver (A) and the skeletal muscle (B) of lactating sows with or without fish oil supplementation on day 20 of lactation. Filled circles (grey = control group, black = fish oil group) represent individual data for each animal. Black lines represent means of individual data for each group (n = 6–10 sows per group). The mean of the control group is set to 1. The mean of the fish oil group is expressed as fold of the control group and numerically indicated next to the black line. Superscript symbol indicates difference from control group (*p<0.05). Abbreviations: CASP1, caspase 1, apoptosis-related cysteine peptidase; IL1B, interleukin 1, beta; NLRP3, NLR family, pyrin domain containing 3; PYCARD, PYD and CARD domain containing.
© Copyright Policy
Related In: Results  -  Collection

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pone.0137684.g009: Effect of fish oil on mRNA concentrations of genes involved in the NLRP3 inflammasome in the liver and skeletal muscle of lactating sows.Relative mRNA concentrations genes involved in the NOD-like receptor P3 (NLRP3) inflammasome in the liver (A) and the skeletal muscle (B) of lactating sows with or without fish oil supplementation on day 20 of lactation. Filled circles (grey = control group, black = fish oil group) represent individual data for each animal. Black lines represent means of individual data for each group (n = 6–10 sows per group). The mean of the control group is set to 1. The mean of the fish oil group is expressed as fold of the control group and numerically indicated next to the black line. Superscript symbol indicates difference from control group (*p<0.05). Abbreviations: CASP1, caspase 1, apoptosis-related cysteine peptidase; IL1B, interleukin 1, beta; NLRP3, NLR family, pyrin domain containing 3; PYCARD, PYD and CARD domain containing.
Mentions: The relative mRNA concentrations of four genes involved in the NLRP3 inflammasome (CASP1, NLRP3, PYCARD, IL1B) in the liver did not differ between the fish oil group and the control group (Fig 9A). In skeletal muscle the relative mRNA concentration of PYCARD was reduced in the fish oil group compared to the control group (p<0.05; Fig 9B), whereas the other genes (CASP1, NLRP3, IL1B) were not different between groups.

Bottom Line: This was demonstrated by decreased mRNA levels of a number of UPR-regulated genes and reduced phosphorylation of PERK and PERK-mediated phosphorylation of eIF2α and IκB in the liver of the fish oil group.In line with this, the plasma levels of acute phase proteins were reduced in the fish oil group, although differences to the control group were not significant.In conclusion, ER stress-induced UPR is present in the liver of lactating sows and fish oil is able to inhibit inflammatory signaling pathways and ER stress-induced UPR in the liver.

View Article: PubMed Central - PubMed

Affiliation: Institute of Animal Nutrition and Nutrition Physiology, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany.

ABSTRACT
Lactating sows have been shown to develop typical signs of an inflammatory condition in the liver during the transition from pregnancy to lactation. Hepatic inflammation is considered critical due to the induction of an acute phase response and the activation of stress signaling pathways like the endoplasmic reticulum (ER) stress-induced unfolded protein response (UPR), both of which impair animal's health and performance. Whether ER stress-induced UPR is also activated in the liver of lactating sows and whether dietary fish oil as a source of anti-inflammatory effects n-3 PUFA is able to attenuate hepatic inflammation and ER stress-induced UPR in the liver of sows is currently unknown. Based on this, two experiments with lactating sows were performed. The first experiment revealed that ER stress-induced UPR occurs also in the liver of sows during lactation. This was evident from the up-regulation of a set of genes regulated by the UPR and numerically increased phosphorylation of the ER stress-transducer PERK and PERK-mediated phosphorylation of eIF2α and IκB. The second experiment showed that fish oil inhibits ER stress-induced UPR in the liver of lactating sows. This was demonstrated by decreased mRNA levels of a number of UPR-regulated genes and reduced phosphorylation of PERK and PERK-mediated phosphorylation of eIF2α and IκB in the liver of the fish oil group. The mRNA levels of various nuclear factor-κB-regulated genes encoding inflammatory mediators and acute phase proteins in the liver of lactating sows were also reduced in the fish oil group. In line with this, the plasma levels of acute phase proteins were reduced in the fish oil group, although differences to the control group were not significant. In conclusion, ER stress-induced UPR is present in the liver of lactating sows and fish oil is able to inhibit inflammatory signaling pathways and ER stress-induced UPR in the liver.

No MeSH data available.


Related in: MedlinePlus