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Enhanced Production of Androst-1,4-Diene-3,17-Dione by Mycobacterium neoaurum JC-12 Using Three-Stage Fermentation Strategy.

Shao M, Zhang X, Rao Z, Xu M, Yang T, Li H, Xu Z - PLoS ONE (2015)

Bottom Line: By applying this strategy, the fermentation duration was decreased from 168 h to 120 h with the ADD productivity increased from 0.071 g/(L·h) to 0.108 g/(L·h).Further, three-stage fermentation by adding phytosterol to improve ADD production at the end of the two-stage fermentation was carried out and the final ADD production reached 18.6 g/L, which is the highest reported ADD production using phytosterol as substrate.Thus, this strategy provides a possible way in enhancing the ADD production in pharmaceutical industry.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, Laboratory of Applied Microorganisms and Metabolic Engineering, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu Province, 214122, P. R. China.

ABSTRACT
To improve the androst-1,4-diene-3,17-dione (ADD) production from phytosterol by Mycobacterium neoaurum JC-12, fructose was firstly found favorable as the initial carbon source to increase the biomass and eliminate the lag phase of M. neoaurum JC-12 in the phytosterol transformation process. Based on this phenomenon, two-stage fermentation by using fructose as the initial carbon source and feeding glucose to maintain strain metabolism was designed. By applying this strategy, the fermentation duration was decreased from 168 h to 120 h with the ADD productivity increased from 0.071 g/(L·h) to 0.108 g/(L·h). Further, three-stage fermentation by adding phytosterol to improve ADD production at the end of the two-stage fermentation was carried out and the final ADD production reached 18.6 g/L, which is the highest reported ADD production using phytosterol as substrate. Thus, this strategy provides a possible way in enhancing the ADD production in pharmaceutical industry.

No MeSH data available.


Related in: MedlinePlus

Effect of the molar ratio of Me-β-CD to substrate on phytosterol conversion efficiency and ADD production.All assays were performed in triplicate, standard deviations of the biological replicates were represented by error bars.
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pone.0137658.g001: Effect of the molar ratio of Me-β-CD to substrate on phytosterol conversion efficiency and ADD production.All assays were performed in triplicate, standard deviations of the biological replicates were represented by error bars.

Mentions: As shown in Fig 1, phytosterol conversion efficiency and ADD production increased accompanied by the increase of Me-β-CD concentrations in some extent. However, the conversion efficiency and ADD production decreased gradually when the Me-β-CD to phytosterol ratio was more than 1.25:1. The results indicated that high Me-β-CD concentration was toxic to M. neoaurum JC-12 and limited the transformation of phytosterol to ADD. Besides, at a high Me-β-CD concentration, phytosterol molecular was enclosed by Me-β-CD and “less accessible” for bioconversion, which in return inhibited the conversion efficiency [25]. The phytosterol conversion efficiency and ADD production were 97.15% and 6.56 g/L under the optimal molar ratio of 1.25:1. While, in contrast, the results of control samples without CDs were only 17.50% and 1.01 g/L, respectively. Therefore, the optimal molar ratio of 1.25:1 was used in the next study.


Enhanced Production of Androst-1,4-Diene-3,17-Dione by Mycobacterium neoaurum JC-12 Using Three-Stage Fermentation Strategy.

Shao M, Zhang X, Rao Z, Xu M, Yang T, Li H, Xu Z - PLoS ONE (2015)

Effect of the molar ratio of Me-β-CD to substrate on phytosterol conversion efficiency and ADD production.All assays were performed in triplicate, standard deviations of the biological replicates were represented by error bars.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4564235&req=5

pone.0137658.g001: Effect of the molar ratio of Me-β-CD to substrate on phytosterol conversion efficiency and ADD production.All assays were performed in triplicate, standard deviations of the biological replicates were represented by error bars.
Mentions: As shown in Fig 1, phytosterol conversion efficiency and ADD production increased accompanied by the increase of Me-β-CD concentrations in some extent. However, the conversion efficiency and ADD production decreased gradually when the Me-β-CD to phytosterol ratio was more than 1.25:1. The results indicated that high Me-β-CD concentration was toxic to M. neoaurum JC-12 and limited the transformation of phytosterol to ADD. Besides, at a high Me-β-CD concentration, phytosterol molecular was enclosed by Me-β-CD and “less accessible” for bioconversion, which in return inhibited the conversion efficiency [25]. The phytosterol conversion efficiency and ADD production were 97.15% and 6.56 g/L under the optimal molar ratio of 1.25:1. While, in contrast, the results of control samples without CDs were only 17.50% and 1.01 g/L, respectively. Therefore, the optimal molar ratio of 1.25:1 was used in the next study.

Bottom Line: By applying this strategy, the fermentation duration was decreased from 168 h to 120 h with the ADD productivity increased from 0.071 g/(L·h) to 0.108 g/(L·h).Further, three-stage fermentation by adding phytosterol to improve ADD production at the end of the two-stage fermentation was carried out and the final ADD production reached 18.6 g/L, which is the highest reported ADD production using phytosterol as substrate.Thus, this strategy provides a possible way in enhancing the ADD production in pharmaceutical industry.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, Laboratory of Applied Microorganisms and Metabolic Engineering, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu Province, 214122, P. R. China.

ABSTRACT
To improve the androst-1,4-diene-3,17-dione (ADD) production from phytosterol by Mycobacterium neoaurum JC-12, fructose was firstly found favorable as the initial carbon source to increase the biomass and eliminate the lag phase of M. neoaurum JC-12 in the phytosterol transformation process. Based on this phenomenon, two-stage fermentation by using fructose as the initial carbon source and feeding glucose to maintain strain metabolism was designed. By applying this strategy, the fermentation duration was decreased from 168 h to 120 h with the ADD productivity increased from 0.071 g/(L·h) to 0.108 g/(L·h). Further, three-stage fermentation by adding phytosterol to improve ADD production at the end of the two-stage fermentation was carried out and the final ADD production reached 18.6 g/L, which is the highest reported ADD production using phytosterol as substrate. Thus, this strategy provides a possible way in enhancing the ADD production in pharmaceutical industry.

No MeSH data available.


Related in: MedlinePlus