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Relationships between Genetic Diversity and Fusarium Toxin Profiles of Winter Wheat Cultivars.

Góral T, Stuper-Szablewska K, Buśko M, Boczkowska M, Walentyn-Góral D, Wiśniewska H, Perkowski J - Plant Pathol. J. (2015)

Bottom Line: Only B trichothecenes (deoxynivalenol, 3-acetyldeoxynivalenol and nivalenol) produced by F. culmorum in grain samples from inoculated plots were present.The results were used to create of matrices of distance between cultivars - for trichothecene concentration in inoculated and naturally infected grain as well as for FHB resistance Correlations between genetic distance versus resistance/mycotoxin profiles were calculated using the Mantel test.Significant but weak relationships were found between genetic distance matrix and FHB resistance or trichothecene concentration in naturally infected grain matrices.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Pathology, Plant Breeding and Acclimatization Institute - National Research Institute, Radzików, 05-870 Błonie, Poland.

ABSTRACT
Fusarium head blight is one of the most important and most common diseases of winter wheat. In order to better understanding this disease and to assess the correlations between different factors, 30 cultivars of this cereal were evaluated in a two-year period. Fusarium head blight resistance was evaluated and the concentration of trichothecene mycotoxins was analysed. Grain samples originated from plants inoculated with Fusarium culmorum and naturally infected with Fusarium species. The genetic distance between the tested cultivars was determined and data were analysed using multivariate data analysis methods. Genetic dissimilarity of wheat cultivars ranged between 0.06 and 0.78. They were grouped into three distinct groups after cluster analysis of genetic distance. Wheat cultivars differed in resistance to spike and kernel infection and in resistance to spread of Fusarium within a spike (type II). Only B trichothecenes (deoxynivalenol, 3-acetyldeoxynivalenol and nivalenol) produced by F. culmorum in grain samples from inoculated plots were present. In control samples trichothecenes of groups A (H-2 toxin, T-2 toxin, T-2 tetraol, T-2 triol, scirpentriol, diacetoxyscirpenol) and B were detected. On the basis of Fusarium head blight assessment and analysis of trichothecene concentration in the grain relationships between morphological characters, Fusarium head blight resistance and mycotoxins in grain of wheat cultivars were examined. The results were used to create of matrices of distance between cultivars - for trichothecene concentration in inoculated and naturally infected grain as well as for FHB resistance Correlations between genetic distance versus resistance/mycotoxin profiles were calculated using the Mantel test. A highly significant correlation between genetic distance and mycotoxin distance was found for the samples inoculated with Fusarium culmorum. Significant but weak relationships were found between genetic distance matrix and FHB resistance or trichothecene concentration in naturally infected grain matrices.

No MeSH data available.


Related in: MedlinePlus

Dendrogram resulting from ISSR-based genetic distance analysis of 30 cultivars of winter wheat, estimated with the Dice genetic similarity coefficient. Cluster analysis was performed using the Ward algorithm. Cultivar numbers correspond to those in Table 1.
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f1-ppj-31-226: Dendrogram resulting from ISSR-based genetic distance analysis of 30 cultivars of winter wheat, estimated with the Dice genetic similarity coefficient. Cluster analysis was performed using the Ward algorithm. Cultivar numbers correspond to those in Table 1.

Mentions: Genetic dissimilarity of cultivars ranged between 0.06 (‘Anthus’ vs. ‘Belenus’) and 0.78 (‘Zyta’ vs. ‘Muszelka’) with the average equal to 0.39. The mean value is much lower than obtained for 172 European winter wheat elite lines by SSR and SNP markers (Würschum et al., 2013). This resulted from the fact that in the cited article lines and cultivars came from more diverse market than one country. Lower value of mean genetic distance in our study is also related to the presence of relatively large group of cultivars with similar genetic make-up as it is clear from cluster analysis and principal coordinate analysis graphs (Figs. 1 and 2). At the same time, higher diversity of cultivars derived by Polish breeding companies was noted in comparison with the foreign ones (0.45 and 0.28 respectively). Of course, this might be the direct result of over-representation of indigenous forms in the tested material but it could also indicate greater richness of well adopt to local climatic condition Polish breeding materials. In general, range of genetic distance among authorised advanced cultivars is specific to the individual countries. Thus the maximum genetic distance of Polish cultivars is higher than obtained for Egyptian and Greek ones (Abdellatif and AbouZeid, 2011), comparable to the Croatian, Pakistani and Chinese cultivars (Maric et al., 2004; Zeb et al., 2009; Zhang et al., 2011) or lower than European elite lines (Würschum et al., 2013). Crops improvement is directly related to genetic diversity, which in wheat had been dramatically narrowed in 1960s and since then has been steadily increasing due to modern breeding (Sears, 1981; van de Wouw et al., 2010). Therefore, incorporation of genetic diversity studies into biotic and abiotic stress resistance breeding became a standard.


Relationships between Genetic Diversity and Fusarium Toxin Profiles of Winter Wheat Cultivars.

Góral T, Stuper-Szablewska K, Buśko M, Boczkowska M, Walentyn-Góral D, Wiśniewska H, Perkowski J - Plant Pathol. J. (2015)

Dendrogram resulting from ISSR-based genetic distance analysis of 30 cultivars of winter wheat, estimated with the Dice genetic similarity coefficient. Cluster analysis was performed using the Ward algorithm. Cultivar numbers correspond to those in Table 1.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4564148&req=5

f1-ppj-31-226: Dendrogram resulting from ISSR-based genetic distance analysis of 30 cultivars of winter wheat, estimated with the Dice genetic similarity coefficient. Cluster analysis was performed using the Ward algorithm. Cultivar numbers correspond to those in Table 1.
Mentions: Genetic dissimilarity of cultivars ranged between 0.06 (‘Anthus’ vs. ‘Belenus’) and 0.78 (‘Zyta’ vs. ‘Muszelka’) with the average equal to 0.39. The mean value is much lower than obtained for 172 European winter wheat elite lines by SSR and SNP markers (Würschum et al., 2013). This resulted from the fact that in the cited article lines and cultivars came from more diverse market than one country. Lower value of mean genetic distance in our study is also related to the presence of relatively large group of cultivars with similar genetic make-up as it is clear from cluster analysis and principal coordinate analysis graphs (Figs. 1 and 2). At the same time, higher diversity of cultivars derived by Polish breeding companies was noted in comparison with the foreign ones (0.45 and 0.28 respectively). Of course, this might be the direct result of over-representation of indigenous forms in the tested material but it could also indicate greater richness of well adopt to local climatic condition Polish breeding materials. In general, range of genetic distance among authorised advanced cultivars is specific to the individual countries. Thus the maximum genetic distance of Polish cultivars is higher than obtained for Egyptian and Greek ones (Abdellatif and AbouZeid, 2011), comparable to the Croatian, Pakistani and Chinese cultivars (Maric et al., 2004; Zeb et al., 2009; Zhang et al., 2011) or lower than European elite lines (Würschum et al., 2013). Crops improvement is directly related to genetic diversity, which in wheat had been dramatically narrowed in 1960s and since then has been steadily increasing due to modern breeding (Sears, 1981; van de Wouw et al., 2010). Therefore, incorporation of genetic diversity studies into biotic and abiotic stress resistance breeding became a standard.

Bottom Line: Only B trichothecenes (deoxynivalenol, 3-acetyldeoxynivalenol and nivalenol) produced by F. culmorum in grain samples from inoculated plots were present.The results were used to create of matrices of distance between cultivars - for trichothecene concentration in inoculated and naturally infected grain as well as for FHB resistance Correlations between genetic distance versus resistance/mycotoxin profiles were calculated using the Mantel test.Significant but weak relationships were found between genetic distance matrix and FHB resistance or trichothecene concentration in naturally infected grain matrices.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Pathology, Plant Breeding and Acclimatization Institute - National Research Institute, Radzików, 05-870 Błonie, Poland.

ABSTRACT
Fusarium head blight is one of the most important and most common diseases of winter wheat. In order to better understanding this disease and to assess the correlations between different factors, 30 cultivars of this cereal were evaluated in a two-year period. Fusarium head blight resistance was evaluated and the concentration of trichothecene mycotoxins was analysed. Grain samples originated from plants inoculated with Fusarium culmorum and naturally infected with Fusarium species. The genetic distance between the tested cultivars was determined and data were analysed using multivariate data analysis methods. Genetic dissimilarity of wheat cultivars ranged between 0.06 and 0.78. They were grouped into three distinct groups after cluster analysis of genetic distance. Wheat cultivars differed in resistance to spike and kernel infection and in resistance to spread of Fusarium within a spike (type II). Only B trichothecenes (deoxynivalenol, 3-acetyldeoxynivalenol and nivalenol) produced by F. culmorum in grain samples from inoculated plots were present. In control samples trichothecenes of groups A (H-2 toxin, T-2 toxin, T-2 tetraol, T-2 triol, scirpentriol, diacetoxyscirpenol) and B were detected. On the basis of Fusarium head blight assessment and analysis of trichothecene concentration in the grain relationships between morphological characters, Fusarium head blight resistance and mycotoxins in grain of wheat cultivars were examined. The results were used to create of matrices of distance between cultivars - for trichothecene concentration in inoculated and naturally infected grain as well as for FHB resistance Correlations between genetic distance versus resistance/mycotoxin profiles were calculated using the Mantel test. A highly significant correlation between genetic distance and mycotoxin distance was found for the samples inoculated with Fusarium culmorum. Significant but weak relationships were found between genetic distance matrix and FHB resistance or trichothecene concentration in naturally infected grain matrices.

No MeSH data available.


Related in: MedlinePlus