Limits...
Nonlinear electrical impedance spectroscopy of viruses using very high electric fields created by nanogap electrodes.

Hatsuki R, Honda A, Kajitani M, Yamamoto T - Front Microbiol (2015)

Bottom Line: Our living sphere is constantly exposed to a wide range of pathogenic viruses, which can be either known, or of novel origin.These preliminary results show that the three virus types can be distinguished and their approximate concentrations determined.Although further studies are required, the proposed nonlinear impedance spectroscopy method may achieve a sensitivity comparable to that of more traditional, but less versatile, virus detection systems.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical and Control Engineering, Tokyo Institute of Technology Tokyo, Japan.

ABSTRACT
Our living sphere is constantly exposed to a wide range of pathogenic viruses, which can be either known, or of novel origin. Currently, there is no methodology for continuously monitoring the environment for viruses in general, much less a methodology that allows the rapid and sensitive identification of a wide variety of viruses responsible for communicable diseases. Traditional approaches, based on PCR and immunodetection systems, only detect known or specifically targeted viruses. We here describe a simple device that can potentially detect any virus between nanogap electrodes using nonlinear impedance spectroscopy. Three test viruses, differing in shape and size, were used to demonstrate the general applicability of this approach: baculovirus, tobacco mosaic virus (TMV), and influenza virus. We show that each of the virus types responded differently in the nanogap to changes in the electric field strength, and the impedance of the virus solutions differed depending both on virus type and virus concentration. These preliminary results show that the three virus types can be distinguished and their approximate concentrations determined. Although further studies are required, the proposed nonlinear impedance spectroscopy method may achieve a sensitivity comparable to that of more traditional, but less versatile, virus detection systems.

No MeSH data available.


Related in: MedlinePlus

Real and imaginary components of measured impedance for (A) Baculovirus, (B) TMV, and (C) Influenza virus.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4563260&req=5

Figure 4: Real and imaginary components of measured impedance for (A) Baculovirus, (B) TMV, and (C) Influenza virus.

Mentions: Our investigation of the impedance dependence on virus concentration showed that the impedance varies with virus concentration in the range 1011–1014 virions/mL for baculovirus, TMV, and influenza virus, as shown in Figure 4. In the figure, the solid and dotted lines represent the real and imaginary impedance components, respectively. For the real component, similar behavior is found for the baculovirus and influenza viruses, as shown in Figure 4A and Figure 4C, respectively. The values decrease moderately with increasing frequency between 100 kHz and 1 MHz, then fall sharply at higher frequency, and also generally increase with increasing sample concentration. In contrast, the imaginary component for both viruses shows a peak near 1 MHz that increases in height with increasing sample concentration. As shown in Figure 4C, however, the trends exhibited by TMV clearly differ from those for the baculovirus and influenza viruses. The real impedance component for TMV peaks at 1 MHz, then decreases rapidly at higher frequency. The values generally increase with increasing sample concentration as with the other two viruses, whereas the imaginary impedance component for TMV tends to decrease up to a frequency of 1 MHz, and then increase to a maximum near 3.9 MHz.


Nonlinear electrical impedance spectroscopy of viruses using very high electric fields created by nanogap electrodes.

Hatsuki R, Honda A, Kajitani M, Yamamoto T - Front Microbiol (2015)

Real and imaginary components of measured impedance for (A) Baculovirus, (B) TMV, and (C) Influenza virus.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4563260&req=5

Figure 4: Real and imaginary components of measured impedance for (A) Baculovirus, (B) TMV, and (C) Influenza virus.
Mentions: Our investigation of the impedance dependence on virus concentration showed that the impedance varies with virus concentration in the range 1011–1014 virions/mL for baculovirus, TMV, and influenza virus, as shown in Figure 4. In the figure, the solid and dotted lines represent the real and imaginary impedance components, respectively. For the real component, similar behavior is found for the baculovirus and influenza viruses, as shown in Figure 4A and Figure 4C, respectively. The values decrease moderately with increasing frequency between 100 kHz and 1 MHz, then fall sharply at higher frequency, and also generally increase with increasing sample concentration. In contrast, the imaginary component for both viruses shows a peak near 1 MHz that increases in height with increasing sample concentration. As shown in Figure 4C, however, the trends exhibited by TMV clearly differ from those for the baculovirus and influenza viruses. The real impedance component for TMV peaks at 1 MHz, then decreases rapidly at higher frequency. The values generally increase with increasing sample concentration as with the other two viruses, whereas the imaginary impedance component for TMV tends to decrease up to a frequency of 1 MHz, and then increase to a maximum near 3.9 MHz.

Bottom Line: Our living sphere is constantly exposed to a wide range of pathogenic viruses, which can be either known, or of novel origin.These preliminary results show that the three virus types can be distinguished and their approximate concentrations determined.Although further studies are required, the proposed nonlinear impedance spectroscopy method may achieve a sensitivity comparable to that of more traditional, but less versatile, virus detection systems.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical and Control Engineering, Tokyo Institute of Technology Tokyo, Japan.

ABSTRACT
Our living sphere is constantly exposed to a wide range of pathogenic viruses, which can be either known, or of novel origin. Currently, there is no methodology for continuously monitoring the environment for viruses in general, much less a methodology that allows the rapid and sensitive identification of a wide variety of viruses responsible for communicable diseases. Traditional approaches, based on PCR and immunodetection systems, only detect known or specifically targeted viruses. We here describe a simple device that can potentially detect any virus between nanogap electrodes using nonlinear impedance spectroscopy. Three test viruses, differing in shape and size, were used to demonstrate the general applicability of this approach: baculovirus, tobacco mosaic virus (TMV), and influenza virus. We show that each of the virus types responded differently in the nanogap to changes in the electric field strength, and the impedance of the virus solutions differed depending both on virus type and virus concentration. These preliminary results show that the three virus types can be distinguished and their approximate concentrations determined. Although further studies are required, the proposed nonlinear impedance spectroscopy method may achieve a sensitivity comparable to that of more traditional, but less versatile, virus detection systems.

No MeSH data available.


Related in: MedlinePlus