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Detection and Significance of CD4+CD25+CD127dim Regulatory T Cells in Individuals with Severe Aplastic Anemia.

Qi W, Ren Y, Fu R, Wang H, Liu C, Shao Z - Turk J Haematol (2015)

Bottom Line: DC subset (myeloid DC/plasmacytoid DC ratio, DC1/DC2 ratio) was 3.08 ± 0.72 in untreated patients, which was higher than recovery patients (1.61 ± 0.49) and normal controls (1.39 ± 0.36) (p<0.05).The percentage of CD4(+)CD25(+)CD127(dim) Tregs in PBL was positively associated with T cell subset (r=0.955, p<0.01), and negatively associated with DC subset (r=-0.765, p<0.01).There were significant positive correlations between CD4(+)CD25(+)CD127(dim) Tregs/PBL and granulocyte counts and RET% (r=0.739, 0.749 respectively, p<0.01).

View Article: PubMed Central - PubMed

ABSTRACT

Introduction: To investigate the relationship between CD4(+)CD25(+)CD127(dim) regulatory T cells (Tregs) and immune imbalance in acquired severe aplastic anemia (SAA).

Methods: The quantity of CD4(+)CD25(+)CD127(dim) Tregs in 44 SAA patients and 23 normal controls were measured by flow cytometry. Correlations between Tregs and T cell subsets, dendritic cell (DC) subsets, granulocyte counts and percentage of reticulocytes (RET%) were analyzed.

Results: The percentage of CD4(+)CD25(+)CD127(dim) Tregs in peripheral blood lymphocytes (PBL) of untreated patients was lower than in recovery patients and normal controls (0.83 ± 0.44% vs 2.91 ± 1.24% and 2.18 ± 0.55%, respectively, p<0.05). The percentage of CD4(+)CD25(+)CD127(dim) Tregs in CD4(+) T lymphocytes of recovery patients was higher than for untreated patients and normal controls (9.39 ± 3.51% vs 7.61 ± 5.3% and 6.83 ± 1.4%, respectively, p<0.05). The percentage of CD4(+) T lymphocytes in PBL of untreated patients was lower than for recovery patients and normal controls (13.55 ± 7.37% vs 31.82 ± 8.43% and 32.12 ± 5.88%, respectively, p<0.05). T cell subset (CD4(+)/CD8(+) ratio) was 0.41 ± 0.24 in untreated patients, which was lower than recovery patients (1.2 ± 0.4) and normal controls (1.11 ± 0.23) (p<0.05). DC subset (myeloid DC/plasmacytoid DC ratio, DC1/DC2 ratio) was 3.08 ± 0.72 in untreated patients, which was higher than recovery patients (1.61 ± 0.49) and normal controls (1.39 ± 0.36) (p<0.05). The percentage of CD4(+)CD25(+)CD127(dim) Tregs in PBL was positively associated with T cell subset (r=0.955, p<0.01), and negatively associated with DC subset (r=-0.765, p<0.01). There were significant positive correlations between CD4(+)CD25(+)CD127(dim) Tregs/PBL and granulocyte counts and RET% (r=0.739, 0.749 respectively, p<0.01).

Discussion and conclusion: The decrease of CD4(+)CD25(+)CD127(dim) Tregs in SAA patients may cause excessive functions of T lymphocytes and thus lead to hematopoiesis failure in SAA.

No MeSH data available.


Related in: MedlinePlus

The quantities of Tregs and CD4+ T cells in untreated severe aplastic anemia patients (SAA), recovery patients (R-SAA), and normal controls (Health). A) The percentage of CD4+CD25+CD127dim Tregs in peripheral blood lymphocytes. B) The percentage of CD4+CD25+CD127dim Tregs in CD4+ T cells. C) The percentage of CD4+ T cells in peripheral blood lymphocytes.
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f1: The quantities of Tregs and CD4+ T cells in untreated severe aplastic anemia patients (SAA), recovery patients (R-SAA), and normal controls (Health). A) The percentage of CD4+CD25+CD127dim Tregs in peripheral blood lymphocytes. B) The percentage of CD4+CD25+CD127dim Tregs in CD4+ T cells. C) The percentage of CD4+ T cells in peripheral blood lymphocytes.

Mentions: The percentage of CD4+CD25+CD127dim Tregs in peripheral blood lymphocytes (PBLs) was 0.83±0.44% in untreated SAA patients, 2.91±1.24% in recovery patients, and 2.18±0.55% in normal controls. The percentage of CD4+CD25+CD127dim Tregs in PBLs in untreated SAA patients was lower than in recovery patients and normal controls (p<0.05), and there was no statistical difference between recovery patients and normal controls (p>0.05). The percentage of CD4+CD25+CD127dim in CD4+ T cells in recovery patients (9.39±3.51%) was higher than in untreated patients (7.61±5.3%) and normal controls (6.83±1.4%) (p<0.05), and there was no statistical difference between recovery patients and normal controls (p>0.05). The percentage of CD4+ T cells in PBLs from untreated patients (13.55±7.37%) was lower than in recovery patients (31.82±8.43%) and normal controls (32.12±5.88%) (p<0.05), while there was no statistical difference between recovery patients and normal controls (p>0.05) (Figure 1).


Detection and Significance of CD4+CD25+CD127dim Regulatory T Cells in Individuals with Severe Aplastic Anemia.

Qi W, Ren Y, Fu R, Wang H, Liu C, Shao Z - Turk J Haematol (2015)

The quantities of Tregs and CD4+ T cells in untreated severe aplastic anemia patients (SAA), recovery patients (R-SAA), and normal controls (Health). A) The percentage of CD4+CD25+CD127dim Tregs in peripheral blood lymphocytes. B) The percentage of CD4+CD25+CD127dim Tregs in CD4+ T cells. C) The percentage of CD4+ T cells in peripheral blood lymphocytes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4563197&req=5

f1: The quantities of Tregs and CD4+ T cells in untreated severe aplastic anemia patients (SAA), recovery patients (R-SAA), and normal controls (Health). A) The percentage of CD4+CD25+CD127dim Tregs in peripheral blood lymphocytes. B) The percentage of CD4+CD25+CD127dim Tregs in CD4+ T cells. C) The percentage of CD4+ T cells in peripheral blood lymphocytes.
Mentions: The percentage of CD4+CD25+CD127dim Tregs in peripheral blood lymphocytes (PBLs) was 0.83±0.44% in untreated SAA patients, 2.91±1.24% in recovery patients, and 2.18±0.55% in normal controls. The percentage of CD4+CD25+CD127dim Tregs in PBLs in untreated SAA patients was lower than in recovery patients and normal controls (p<0.05), and there was no statistical difference between recovery patients and normal controls (p>0.05). The percentage of CD4+CD25+CD127dim in CD4+ T cells in recovery patients (9.39±3.51%) was higher than in untreated patients (7.61±5.3%) and normal controls (6.83±1.4%) (p<0.05), and there was no statistical difference between recovery patients and normal controls (p>0.05). The percentage of CD4+ T cells in PBLs from untreated patients (13.55±7.37%) was lower than in recovery patients (31.82±8.43%) and normal controls (32.12±5.88%) (p<0.05), while there was no statistical difference between recovery patients and normal controls (p>0.05) (Figure 1).

Bottom Line: DC subset (myeloid DC/plasmacytoid DC ratio, DC1/DC2 ratio) was 3.08 ± 0.72 in untreated patients, which was higher than recovery patients (1.61 ± 0.49) and normal controls (1.39 ± 0.36) (p<0.05).The percentage of CD4(+)CD25(+)CD127(dim) Tregs in PBL was positively associated with T cell subset (r=0.955, p<0.01), and negatively associated with DC subset (r=-0.765, p<0.01).There were significant positive correlations between CD4(+)CD25(+)CD127(dim) Tregs/PBL and granulocyte counts and RET% (r=0.739, 0.749 respectively, p<0.01).

View Article: PubMed Central - PubMed

ABSTRACT

Introduction: To investigate the relationship between CD4(+)CD25(+)CD127(dim) regulatory T cells (Tregs) and immune imbalance in acquired severe aplastic anemia (SAA).

Methods: The quantity of CD4(+)CD25(+)CD127(dim) Tregs in 44 SAA patients and 23 normal controls were measured by flow cytometry. Correlations between Tregs and T cell subsets, dendritic cell (DC) subsets, granulocyte counts and percentage of reticulocytes (RET%) were analyzed.

Results: The percentage of CD4(+)CD25(+)CD127(dim) Tregs in peripheral blood lymphocytes (PBL) of untreated patients was lower than in recovery patients and normal controls (0.83 ± 0.44% vs 2.91 ± 1.24% and 2.18 ± 0.55%, respectively, p<0.05). The percentage of CD4(+)CD25(+)CD127(dim) Tregs in CD4(+) T lymphocytes of recovery patients was higher than for untreated patients and normal controls (9.39 ± 3.51% vs 7.61 ± 5.3% and 6.83 ± 1.4%, respectively, p<0.05). The percentage of CD4(+) T lymphocytes in PBL of untreated patients was lower than for recovery patients and normal controls (13.55 ± 7.37% vs 31.82 ± 8.43% and 32.12 ± 5.88%, respectively, p<0.05). T cell subset (CD4(+)/CD8(+) ratio) was 0.41 ± 0.24 in untreated patients, which was lower than recovery patients (1.2 ± 0.4) and normal controls (1.11 ± 0.23) (p<0.05). DC subset (myeloid DC/plasmacytoid DC ratio, DC1/DC2 ratio) was 3.08 ± 0.72 in untreated patients, which was higher than recovery patients (1.61 ± 0.49) and normal controls (1.39 ± 0.36) (p<0.05). The percentage of CD4(+)CD25(+)CD127(dim) Tregs in PBL was positively associated with T cell subset (r=0.955, p<0.01), and negatively associated with DC subset (r=-0.765, p<0.01). There were significant positive correlations between CD4(+)CD25(+)CD127(dim) Tregs/PBL and granulocyte counts and RET% (r=0.739, 0.749 respectively, p<0.01).

Discussion and conclusion: The decrease of CD4(+)CD25(+)CD127(dim) Tregs in SAA patients may cause excessive functions of T lymphocytes and thus lead to hematopoiesis failure in SAA.

No MeSH data available.


Related in: MedlinePlus