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Proliferative and Glycolytic Assessment of the Whole-Body Bone Marrow Compartment.

Goryawala M, Adjoua M, Güleç S - Mol Imaging Radionucl Ther (2015)

Bottom Line: RBV obtained using 18F-FLT in males were found to have high correlation with measured weight (R2=0.61) and BMI (R2=0.70).Results also show that spinal activity SUV threshold for red BM segmentation is significantly different between 18F-FLT PET and 18F-FDG PET (p<0.05).Abstract available from the publisher.

View Article: PubMed Central - PubMed

Affiliation: Seza Güleç MD, Florida International University Herbert Wertheim College, Department of Surgery, Miami, USA Phone: +17866930821 E-mail: sgulec@fiu.edu.

ABSTRACT

Objective: Quantitative assessment of active bone marrow (BM) in vivo is yet to be well-defined. This study aims to compare total body BM volume estimations obtained from use of both18F-FLT PET/CT and 18F-FDG PET/CT in order to consolidate higher cellular proliferation rates with imaging the highly active red BM in pancreatic cancer.

Methods: This phase I pilot study includes seven patients with pancreatic cancers who underwent both 18F-FLT and 18F-FDG imaging each acquired within a week's duration. A CT-based classifier is used for segmenting bone into cortical and trabecular regions. The total BM volume is determined through statistical thresholding on PET activity found within the trabecular bone.

Results: Results showed that 18F-FLT measures of red BM volume (RBV) were higher than those obtained from 18F-FDG (∆=89.21 ml). RBV obtained using 18F-FLT in males were found to have high correlation with measured weight (R2=0.61) and BMI (R2=0.70). The red BM fraction obtained from 18F-FLT was significantly different between males and females, with females showing much higher red bone matter within the trabecular bone (p<0.05). In contrast to 18F-FLT, 18F-FDG BM measurements showed that RBV was significantly different between males and females (p<0.05). Results also show that spinal activity SUV threshold for red BM segmentation is significantly different between 18F-FLT PET and 18F-FDG PET (p<0.05).

Conclusion: By combining 18F-FLT-PET and 18F-FDG-PET, this study provides useful insights for in vivo BM estimation through its proliferative and glycolytic activities.

No MeSH data available.


Related in: MedlinePlus

Full body images at various steps of the BM extraction process with the top row showing 18F-FLT and bottom row showing 18F-FDG results. (A) CT image (B) Extracted cortical bone using probabilistic LDA (C) Extracted trabecular bone following probabilistic LDA (D) Activity in trabecular bone in thoracic and lumbar vertebrae regions (E) Activity in trabecular bone in the rest of the body excluding thoracic and lumbar vertebrae regions (F) Thresholded BM map generated after applying a statistical thresholding on PET image. (G) 3D rendering of BM in the entire body, with red regions showing red BM and yellow regions corresponding to yellow BM. The cortical bone overlay is showing using a transparent white overlay
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f2: Full body images at various steps of the BM extraction process with the top row showing 18F-FLT and bottom row showing 18F-FDG results. (A) CT image (B) Extracted cortical bone using probabilistic LDA (C) Extracted trabecular bone following probabilistic LDA (D) Activity in trabecular bone in thoracic and lumbar vertebrae regions (E) Activity in trabecular bone in the rest of the body excluding thoracic and lumbar vertebrae regions (F) Thresholded BM map generated after applying a statistical thresholding on PET image. (G) 3D rendering of BM in the entire body, with red regions showing red BM and yellow regions corresponding to yellow BM. The cortical bone overlay is showing using a transparent white overlay

Mentions: Finally, the red BM fraction was found to be loosely correlated with the measured weight (R2=0.49; Figure 4A) and well correlated to the measured height (R2=0.68) and the ideal body weight (R2=0.73; Figure 4B). It was also significantly different between males and females, with females showing much higher red bone matter within trabecular bone (p<0.05).


Proliferative and Glycolytic Assessment of the Whole-Body Bone Marrow Compartment.

Goryawala M, Adjoua M, Güleç S - Mol Imaging Radionucl Ther (2015)

Full body images at various steps of the BM extraction process with the top row showing 18F-FLT and bottom row showing 18F-FDG results. (A) CT image (B) Extracted cortical bone using probabilistic LDA (C) Extracted trabecular bone following probabilistic LDA (D) Activity in trabecular bone in thoracic and lumbar vertebrae regions (E) Activity in trabecular bone in the rest of the body excluding thoracic and lumbar vertebrae regions (F) Thresholded BM map generated after applying a statistical thresholding on PET image. (G) 3D rendering of BM in the entire body, with red regions showing red BM and yellow regions corresponding to yellow BM. The cortical bone overlay is showing using a transparent white overlay
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4563173&req=5

f2: Full body images at various steps of the BM extraction process with the top row showing 18F-FLT and bottom row showing 18F-FDG results. (A) CT image (B) Extracted cortical bone using probabilistic LDA (C) Extracted trabecular bone following probabilistic LDA (D) Activity in trabecular bone in thoracic and lumbar vertebrae regions (E) Activity in trabecular bone in the rest of the body excluding thoracic and lumbar vertebrae regions (F) Thresholded BM map generated after applying a statistical thresholding on PET image. (G) 3D rendering of BM in the entire body, with red regions showing red BM and yellow regions corresponding to yellow BM. The cortical bone overlay is showing using a transparent white overlay
Mentions: Finally, the red BM fraction was found to be loosely correlated with the measured weight (R2=0.49; Figure 4A) and well correlated to the measured height (R2=0.68) and the ideal body weight (R2=0.73; Figure 4B). It was also significantly different between males and females, with females showing much higher red bone matter within trabecular bone (p<0.05).

Bottom Line: RBV obtained using 18F-FLT in males were found to have high correlation with measured weight (R2=0.61) and BMI (R2=0.70).Results also show that spinal activity SUV threshold for red BM segmentation is significantly different between 18F-FLT PET and 18F-FDG PET (p<0.05).Abstract available from the publisher.

View Article: PubMed Central - PubMed

Affiliation: Seza Güleç MD, Florida International University Herbert Wertheim College, Department of Surgery, Miami, USA Phone: +17866930821 E-mail: sgulec@fiu.edu.

ABSTRACT

Objective: Quantitative assessment of active bone marrow (BM) in vivo is yet to be well-defined. This study aims to compare total body BM volume estimations obtained from use of both18F-FLT PET/CT and 18F-FDG PET/CT in order to consolidate higher cellular proliferation rates with imaging the highly active red BM in pancreatic cancer.

Methods: This phase I pilot study includes seven patients with pancreatic cancers who underwent both 18F-FLT and 18F-FDG imaging each acquired within a week's duration. A CT-based classifier is used for segmenting bone into cortical and trabecular regions. The total BM volume is determined through statistical thresholding on PET activity found within the trabecular bone.

Results: Results showed that 18F-FLT measures of red BM volume (RBV) were higher than those obtained from 18F-FDG (∆=89.21 ml). RBV obtained using 18F-FLT in males were found to have high correlation with measured weight (R2=0.61) and BMI (R2=0.70). The red BM fraction obtained from 18F-FLT was significantly different between males and females, with females showing much higher red bone matter within the trabecular bone (p<0.05). In contrast to 18F-FLT, 18F-FDG BM measurements showed that RBV was significantly different between males and females (p<0.05). Results also show that spinal activity SUV threshold for red BM segmentation is significantly different between 18F-FLT PET and 18F-FDG PET (p<0.05).

Conclusion: By combining 18F-FLT-PET and 18F-FDG-PET, this study provides useful insights for in vivo BM estimation through its proliferative and glycolytic activities.

No MeSH data available.


Related in: MedlinePlus