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A case study on the genetic origin of the high oleic acid trait through FAD2-1 DNA sequence variation in safflower (Carthamus tinctorius L.).

Rapson S, Wu M, Okada S, Das A, Shrestha P, Zhou XR, Wood C, Green A, Singh S, Liu Q - Front Plant Sci (2015)

Bottom Line: The ol allele was found to be a defective microsomal oleate desaturase FAD2-1.It is from this gene that FAD2-1Δ was derived more recently.Identification and characterization of the genetic origin and diversity of FAD2-1 could aid safflower breeders in reducing population size and generations required for the development of new high oleic acid varieties by using perfect molecular marker-assisted selection.

View Article: PubMed Central - PubMed

Affiliation: Commonwealth Scientific and Industrial Research Organization Agriculture Canberra, ACT, Australia.

ABSTRACT
The safflower (Carthamus tinctorius L.) is considered a strongly domesticated species with a long history of cultivation. The hybridization of safflower with its wild relatives has played an important role in the evolution of cultivars and is of particular interest with regards to their production of high quality edible oils. Original safflower varieties were all rich in linoleic acid, while varieties rich in oleic acid have risen to prominence in recent decades. The high oleic acid trait is controlled by a partially recessive allele ol at a single locus OL. The ol allele was found to be a defective microsomal oleate desaturase FAD2-1. Here we present DNA sequence data and Southern blot analysis suggesting that there has been an ancient hybridization and introgression of the FAD2-1 gene into C. tinctorius from its wild relative C. palaestinus. It is from this gene that FAD2-1Δ was derived more recently. Identification and characterization of the genetic origin and diversity of FAD2-1 could aid safflower breeders in reducing population size and generations required for the development of new high oleic acid varieties by using perfect molecular marker-assisted selection.

No MeSH data available.


Related in: MedlinePlus

Southern blot FAD2-2 digestion radiographs (top using enzyme BglII, bottom using enzyme HindIII). Samples are in order according to their listing in Table 1 starting with PI 209295 and ending with PI 426488. , C. tinctorius group A; , C. tinctorius group B; , C. palaestinus; , C. oxyacantha.
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Figure 5: Southern blot FAD2-2 digestion radiographs (top using enzyme BglII, bottom using enzyme HindIII). Samples are in order according to their listing in Table 1 starting with PI 209295 and ending with PI 426488. , C. tinctorius group A; , C. tinctorius group B; , C. palaestinus; , C. oxyacantha.

Mentions: However, such an observation was not made in the FAD2-2 RFLP patterns as illustrated in Figure 5. Except PI 292000, the other 16 C. tinctorius accessions (labeled with yellow or red dots) shared the same RFLP pattern (a band of approximately 3.0 kb) with C. palaestinus (labeled with a green square) and two C. oxycanthus (labeled with blue crosses) in the BglII digestion, whereas the C. palaestius showed a clear distinction from all the 17 C. tinctorius accessions, but shared a band of approximately 12.0 kb with all four C. oxycanthus in the HindIII digestion.


A case study on the genetic origin of the high oleic acid trait through FAD2-1 DNA sequence variation in safflower (Carthamus tinctorius L.).

Rapson S, Wu M, Okada S, Das A, Shrestha P, Zhou XR, Wood C, Green A, Singh S, Liu Q - Front Plant Sci (2015)

Southern blot FAD2-2 digestion radiographs (top using enzyme BglII, bottom using enzyme HindIII). Samples are in order according to their listing in Table 1 starting with PI 209295 and ending with PI 426488. , C. tinctorius group A; , C. tinctorius group B; , C. palaestinus; , C. oxyacantha.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4563165&req=5

Figure 5: Southern blot FAD2-2 digestion radiographs (top using enzyme BglII, bottom using enzyme HindIII). Samples are in order according to their listing in Table 1 starting with PI 209295 and ending with PI 426488. , C. tinctorius group A; , C. tinctorius group B; , C. palaestinus; , C. oxyacantha.
Mentions: However, such an observation was not made in the FAD2-2 RFLP patterns as illustrated in Figure 5. Except PI 292000, the other 16 C. tinctorius accessions (labeled with yellow or red dots) shared the same RFLP pattern (a band of approximately 3.0 kb) with C. palaestinus (labeled with a green square) and two C. oxycanthus (labeled with blue crosses) in the BglII digestion, whereas the C. palaestius showed a clear distinction from all the 17 C. tinctorius accessions, but shared a band of approximately 12.0 kb with all four C. oxycanthus in the HindIII digestion.

Bottom Line: The ol allele was found to be a defective microsomal oleate desaturase FAD2-1.It is from this gene that FAD2-1Δ was derived more recently.Identification and characterization of the genetic origin and diversity of FAD2-1 could aid safflower breeders in reducing population size and generations required for the development of new high oleic acid varieties by using perfect molecular marker-assisted selection.

View Article: PubMed Central - PubMed

Affiliation: Commonwealth Scientific and Industrial Research Organization Agriculture Canberra, ACT, Australia.

ABSTRACT
The safflower (Carthamus tinctorius L.) is considered a strongly domesticated species with a long history of cultivation. The hybridization of safflower with its wild relatives has played an important role in the evolution of cultivars and is of particular interest with regards to their production of high quality edible oils. Original safflower varieties were all rich in linoleic acid, while varieties rich in oleic acid have risen to prominence in recent decades. The high oleic acid trait is controlled by a partially recessive allele ol at a single locus OL. The ol allele was found to be a defective microsomal oleate desaturase FAD2-1. Here we present DNA sequence data and Southern blot analysis suggesting that there has been an ancient hybridization and introgression of the FAD2-1 gene into C. tinctorius from its wild relative C. palaestinus. It is from this gene that FAD2-1Δ was derived more recently. Identification and characterization of the genetic origin and diversity of FAD2-1 could aid safflower breeders in reducing population size and generations required for the development of new high oleic acid varieties by using perfect molecular marker-assisted selection.

No MeSH data available.


Related in: MedlinePlus