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A case study on the genetic origin of the high oleic acid trait through FAD2-1 DNA sequence variation in safflower (Carthamus tinctorius L.).

Rapson S, Wu M, Okada S, Das A, Shrestha P, Zhou XR, Wood C, Green A, Singh S, Liu Q - Front Plant Sci (2015)

Bottom Line: The ol allele was found to be a defective microsomal oleate desaturase FAD2-1.It is from this gene that FAD2-1Δ was derived more recently.Identification and characterization of the genetic origin and diversity of FAD2-1 could aid safflower breeders in reducing population size and generations required for the development of new high oleic acid varieties by using perfect molecular marker-assisted selection.

View Article: PubMed Central - PubMed

Affiliation: Commonwealth Scientific and Industrial Research Organization Agriculture Canberra, ACT, Australia.

ABSTRACT
The safflower (Carthamus tinctorius L.) is considered a strongly domesticated species with a long history of cultivation. The hybridization of safflower with its wild relatives has played an important role in the evolution of cultivars and is of particular interest with regards to their production of high quality edible oils. Original safflower varieties were all rich in linoleic acid, while varieties rich in oleic acid have risen to prominence in recent decades. The high oleic acid trait is controlled by a partially recessive allele ol at a single locus OL. The ol allele was found to be a defective microsomal oleate desaturase FAD2-1. Here we present DNA sequence data and Southern blot analysis suggesting that there has been an ancient hybridization and introgression of the FAD2-1 gene into C. tinctorius from its wild relative C. palaestinus. It is from this gene that FAD2-1Δ was derived more recently. Identification and characterization of the genetic origin and diversity of FAD2-1 could aid safflower breeders in reducing population size and generations required for the development of new high oleic acid varieties by using perfect molecular marker-assisted selection.

No MeSH data available.


Related in: MedlinePlus

Southern blot FAD2-1 digestion radiographs (top using enzyme BglII, bottom using enzyme EcoRV). Samples are in order according to their listing in Table 1 starting with PI 209295 and ending with PI 426488. , C. tinctorius group A; , C. tinctorius group B; , C. palaestinus; , C. oxyacantha.
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Figure 4: Southern blot FAD2-1 digestion radiographs (top using enzyme BglII, bottom using enzyme EcoRV). Samples are in order according to their listing in Table 1 starting with PI 209295 and ending with PI 426488. , C. tinctorius group A; , C. tinctorius group B; , C. palaestinus; , C. oxyacantha.

Mentions: DNA blot analysis of FAD2-1 using enzymes BglII and EcoRV consistently showed three distinct restriction fragment length polymorphism (RFLP) patterns in C. tinctorius. Group 1 consisting of 5 accessions, as labeled by a yellow dot in Figure 4, showed a single band of approximately 6.0 kb by BglII digestion, and 2.5 kb by EcoRV digestion. Group 2 consisting of 11 other accessions, as labeled by Red dots, showed a single band of approximately 4.0 kb in length by BglII digestion, and 7.0 kb by EcoRV digestion. Group three is a single accession, PI 271070 labeled by a yellow dot, showed two bands in either restriction enzyme digestion, combining the RFLP pattern of both group 1 and group 2. This particular plant was likely a heterozygous hybrid between groups 1 and 2 of C. tinctorius. Of particular interest, in both BglII and EcoRV digestions, C. palaestinus (labeled by a green square) showed the exactly same RFLP pattern as that of the Group 2 in C. tinctorius. This is consistent with the sequence analysis of FAD2-1 intron, which showed higher sequence similarity between group 2 C. tinctorius and C. palaestinus, distinct from group 1 C. tinctorius. In both restriction enzyme digestions, the four C. oxycanthus accessions showed distinct patterns from either C. tinctorius or C. palaestinus.


A case study on the genetic origin of the high oleic acid trait through FAD2-1 DNA sequence variation in safflower (Carthamus tinctorius L.).

Rapson S, Wu M, Okada S, Das A, Shrestha P, Zhou XR, Wood C, Green A, Singh S, Liu Q - Front Plant Sci (2015)

Southern blot FAD2-1 digestion radiographs (top using enzyme BglII, bottom using enzyme EcoRV). Samples are in order according to their listing in Table 1 starting with PI 209295 and ending with PI 426488. , C. tinctorius group A; , C. tinctorius group B; , C. palaestinus; , C. oxyacantha.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4563165&req=5

Figure 4: Southern blot FAD2-1 digestion radiographs (top using enzyme BglII, bottom using enzyme EcoRV). Samples are in order according to their listing in Table 1 starting with PI 209295 and ending with PI 426488. , C. tinctorius group A; , C. tinctorius group B; , C. palaestinus; , C. oxyacantha.
Mentions: DNA blot analysis of FAD2-1 using enzymes BglII and EcoRV consistently showed three distinct restriction fragment length polymorphism (RFLP) patterns in C. tinctorius. Group 1 consisting of 5 accessions, as labeled by a yellow dot in Figure 4, showed a single band of approximately 6.0 kb by BglII digestion, and 2.5 kb by EcoRV digestion. Group 2 consisting of 11 other accessions, as labeled by Red dots, showed a single band of approximately 4.0 kb in length by BglII digestion, and 7.0 kb by EcoRV digestion. Group three is a single accession, PI 271070 labeled by a yellow dot, showed two bands in either restriction enzyme digestion, combining the RFLP pattern of both group 1 and group 2. This particular plant was likely a heterozygous hybrid between groups 1 and 2 of C. tinctorius. Of particular interest, in both BglII and EcoRV digestions, C. palaestinus (labeled by a green square) showed the exactly same RFLP pattern as that of the Group 2 in C. tinctorius. This is consistent with the sequence analysis of FAD2-1 intron, which showed higher sequence similarity between group 2 C. tinctorius and C. palaestinus, distinct from group 1 C. tinctorius. In both restriction enzyme digestions, the four C. oxycanthus accessions showed distinct patterns from either C. tinctorius or C. palaestinus.

Bottom Line: The ol allele was found to be a defective microsomal oleate desaturase FAD2-1.It is from this gene that FAD2-1Δ was derived more recently.Identification and characterization of the genetic origin and diversity of FAD2-1 could aid safflower breeders in reducing population size and generations required for the development of new high oleic acid varieties by using perfect molecular marker-assisted selection.

View Article: PubMed Central - PubMed

Affiliation: Commonwealth Scientific and Industrial Research Organization Agriculture Canberra, ACT, Australia.

ABSTRACT
The safflower (Carthamus tinctorius L.) is considered a strongly domesticated species with a long history of cultivation. The hybridization of safflower with its wild relatives has played an important role in the evolution of cultivars and is of particular interest with regards to their production of high quality edible oils. Original safflower varieties were all rich in linoleic acid, while varieties rich in oleic acid have risen to prominence in recent decades. The high oleic acid trait is controlled by a partially recessive allele ol at a single locus OL. The ol allele was found to be a defective microsomal oleate desaturase FAD2-1. Here we present DNA sequence data and Southern blot analysis suggesting that there has been an ancient hybridization and introgression of the FAD2-1 gene into C. tinctorius from its wild relative C. palaestinus. It is from this gene that FAD2-1Δ was derived more recently. Identification and characterization of the genetic origin and diversity of FAD2-1 could aid safflower breeders in reducing population size and generations required for the development of new high oleic acid varieties by using perfect molecular marker-assisted selection.

No MeSH data available.


Related in: MedlinePlus