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Post-Exercise Skeletal Muscle Glycogen Related to Plasma Cytokines and Muscle IL-6 Protein Content, but not Muscle Cytokine mRNA Expression.

Nieman DC, Zwetsloot KA, Meaney MP, Lomiwes DD, Hurst SM, Hurst RD - Front Nutr (2015)

Bottom Line: Participants experienced a 35.3 ± 4.2% decrease (P < 0.001) in skeletal muscle glycogen content (67.5 ± 2.8 to 44.3 ± 3.7 mmol⋅kg(-1) wet weight).Skeletal muscle IL-6, IL-8, and MCP-1 protein content increased 35.8 ± 10.6, 80.6 ± 12.1, and 105 ± 17.9%, respectively (all, P ≤ 0.005).Plasma IL-6, IL-8, and MCP-1 increased 47.1 ± 10.0-, 2.6 ± 0.3-, and 1.6 ± 0.1-fold, respectively (all, P < 0.001).

View Article: PubMed Central - PubMed

Affiliation: Appalachian State University , Kannapolis, NC , USA.

ABSTRACT

Objectives: The purpose of this study was to correlate post-exercise muscle glycogen levels with changes in plasma cytokine, and muscle mRNA cytokine expression and protein content.

Methods: Twenty-four male runners (age 36.5 ± 1.8 years, VO2max 60.0 ± 1.5 mL⋅kg(-1) ⋅ min(-1)) ran twice (separated by 4 weeks) on treadmills to exhaustion at 70% VO2max (average time and distance of 2.24 ± 0.09 h and 24.9 ± 1.1 km). Muscle biopsies from the vastus lateralis and blood samples were collected before and after each run, with IL-6, IL-8, and MCP-1 measured in muscle (mRNA and protein) and plasma. Data from the two runs were averaged.

Results: Participants experienced a 35.3 ± 4.2% decrease (P < 0.001) in skeletal muscle glycogen content (67.5 ± 2.8 to 44.3 ± 3.7 mmol⋅kg(-1) wet weight). Muscle mRNA expression for IL-6, IL-8, and MCP-1 increased 7.34 ± 0.90-, 13.9 ± 2.3-, and 4.10 ± 0.60-fold, respectively (all, P < 0.001). Skeletal muscle IL-6, IL-8, and MCP-1 protein content increased 35.8 ± 10.6, 80.6 ± 12.1, and 105 ± 17.9%, respectively (all, P ≤ 0.005). Plasma IL-6, IL-8, and MCP-1 increased 47.1 ± 10.0-, 2.6 ± 0.3-, and 1.6 ± 0.1-fold, respectively (all, P < 0.001). Post-exercise muscle glycogen concentrations were negatively correlated with run time to exhaustion (r = -0.70, P < 0.001), and changes in muscle IL-6 protein content (r = -0.44, P = 0.049), plasma IL-6 (r = -0.72, P < 0.001), IL-8 (r = -0.60, P = 0.002), and MCP-1 (r = -0.589, P = 0.002), but not with changes in muscle IL-8 and MCP-1 protein content, or muscle mRNA expression for IL-6, IL-8, and MCP-1.

Conclusion: Prolonged and intensive running increased muscle mRNA expression, muscle protein content, and plasma levels for IL-6, IL-8, and MCP-1, and post-run muscle glycogen levels were most strongly related to plasma cytokine levels.

No MeSH data available.


Related in: MedlinePlus

Correlations between post-exercise skeletal muscle glycogen content and change in plasma (A) IL-6 (r = −0.718, P < 0.001), (B) IL-8 (r = −0.604, P = 0.002), and (C) MCP-1 (r = −0.589, P = 0.002).
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Figure 4: Correlations between post-exercise skeletal muscle glycogen content and change in plasma (A) IL-6 (r = −0.718, P < 0.001), (B) IL-8 (r = −0.604, P = 0.002), and (C) MCP-1 (r = −0.589, P = 0.002).

Mentions: Correlation statistics were calculated for pre-run, post-run, and absolute change in muscle glycogen with the outcome measures included in this study, and the strongest correlations (when significant) were found when using post-run glycogen values. Significant inverse correlations were measured between post-run glycogen levels and running time (r = −0.70, P < 0.001) and distance (r = −0.609, P = 0.002), but not with muscle mRNA expression for MCP-1 (r = −0.032, P = 0.882), IL-8 (r = −0.349, P = 0.095), or IL-6 (r = −0.211, P = 0.321). Post-run glycogen levels were negatively related to muscle IL-6 protein content (r = −0.55, P = 0.049), but not significantly to muscle IL-8 (r = −0.36, P = 0.120) or MCP-1 (r = −0.33, P = 0.160) protein content. Post-run glycogen levels were negatively related to change in plasma IL-6 (r = −0.718, P < 0.001), IL-8 (r = −0.604, P = 0.002), and MCP-1 (r = −0.589, P = 0.002) (Figure 4), but not to change in serum cortisol (r = 0.011, P = 0.959) or plasma epinephrine (r = 0.15, P = 0.478) (correlation figures not shown). Changes in muscle and plasma IL-6 levels were significantly related (r = 0.73, P < 0.001) (Figure 5), but not as strongly for muscle and plasma IL-8 (r = 0.40, P = 0.080) or MCP-1 (r = 0.413, P = 0.071) (correlation figures not shown). Changes in serum cortisol and plasma epinephrine were not significantly related to changes in muscle IL-6, IL-8, or MCP-1 mRNA or protein levels (all, P > 0.05) (correlation figures not shown).


Post-Exercise Skeletal Muscle Glycogen Related to Plasma Cytokines and Muscle IL-6 Protein Content, but not Muscle Cytokine mRNA Expression.

Nieman DC, Zwetsloot KA, Meaney MP, Lomiwes DD, Hurst SM, Hurst RD - Front Nutr (2015)

Correlations between post-exercise skeletal muscle glycogen content and change in plasma (A) IL-6 (r = −0.718, P < 0.001), (B) IL-8 (r = −0.604, P = 0.002), and (C) MCP-1 (r = −0.589, P = 0.002).
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Related In: Results  -  Collection

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Figure 4: Correlations between post-exercise skeletal muscle glycogen content and change in plasma (A) IL-6 (r = −0.718, P < 0.001), (B) IL-8 (r = −0.604, P = 0.002), and (C) MCP-1 (r = −0.589, P = 0.002).
Mentions: Correlation statistics were calculated for pre-run, post-run, and absolute change in muscle glycogen with the outcome measures included in this study, and the strongest correlations (when significant) were found when using post-run glycogen values. Significant inverse correlations were measured between post-run glycogen levels and running time (r = −0.70, P < 0.001) and distance (r = −0.609, P = 0.002), but not with muscle mRNA expression for MCP-1 (r = −0.032, P = 0.882), IL-8 (r = −0.349, P = 0.095), or IL-6 (r = −0.211, P = 0.321). Post-run glycogen levels were negatively related to muscle IL-6 protein content (r = −0.55, P = 0.049), but not significantly to muscle IL-8 (r = −0.36, P = 0.120) or MCP-1 (r = −0.33, P = 0.160) protein content. Post-run glycogen levels were negatively related to change in plasma IL-6 (r = −0.718, P < 0.001), IL-8 (r = −0.604, P = 0.002), and MCP-1 (r = −0.589, P = 0.002) (Figure 4), but not to change in serum cortisol (r = 0.011, P = 0.959) or plasma epinephrine (r = 0.15, P = 0.478) (correlation figures not shown). Changes in muscle and plasma IL-6 levels were significantly related (r = 0.73, P < 0.001) (Figure 5), but not as strongly for muscle and plasma IL-8 (r = 0.40, P = 0.080) or MCP-1 (r = 0.413, P = 0.071) (correlation figures not shown). Changes in serum cortisol and plasma epinephrine were not significantly related to changes in muscle IL-6, IL-8, or MCP-1 mRNA or protein levels (all, P > 0.05) (correlation figures not shown).

Bottom Line: Participants experienced a 35.3 ± 4.2% decrease (P < 0.001) in skeletal muscle glycogen content (67.5 ± 2.8 to 44.3 ± 3.7 mmol⋅kg(-1) wet weight).Skeletal muscle IL-6, IL-8, and MCP-1 protein content increased 35.8 ± 10.6, 80.6 ± 12.1, and 105 ± 17.9%, respectively (all, P ≤ 0.005).Plasma IL-6, IL-8, and MCP-1 increased 47.1 ± 10.0-, 2.6 ± 0.3-, and 1.6 ± 0.1-fold, respectively (all, P < 0.001).

View Article: PubMed Central - PubMed

Affiliation: Appalachian State University , Kannapolis, NC , USA.

ABSTRACT

Objectives: The purpose of this study was to correlate post-exercise muscle glycogen levels with changes in plasma cytokine, and muscle mRNA cytokine expression and protein content.

Methods: Twenty-four male runners (age 36.5 ± 1.8 years, VO2max 60.0 ± 1.5 mL⋅kg(-1) ⋅ min(-1)) ran twice (separated by 4 weeks) on treadmills to exhaustion at 70% VO2max (average time and distance of 2.24 ± 0.09 h and 24.9 ± 1.1 km). Muscle biopsies from the vastus lateralis and blood samples were collected before and after each run, with IL-6, IL-8, and MCP-1 measured in muscle (mRNA and protein) and plasma. Data from the two runs were averaged.

Results: Participants experienced a 35.3 ± 4.2% decrease (P < 0.001) in skeletal muscle glycogen content (67.5 ± 2.8 to 44.3 ± 3.7 mmol⋅kg(-1) wet weight). Muscle mRNA expression for IL-6, IL-8, and MCP-1 increased 7.34 ± 0.90-, 13.9 ± 2.3-, and 4.10 ± 0.60-fold, respectively (all, P < 0.001). Skeletal muscle IL-6, IL-8, and MCP-1 protein content increased 35.8 ± 10.6, 80.6 ± 12.1, and 105 ± 17.9%, respectively (all, P ≤ 0.005). Plasma IL-6, IL-8, and MCP-1 increased 47.1 ± 10.0-, 2.6 ± 0.3-, and 1.6 ± 0.1-fold, respectively (all, P < 0.001). Post-exercise muscle glycogen concentrations were negatively correlated with run time to exhaustion (r = -0.70, P < 0.001), and changes in muscle IL-6 protein content (r = -0.44, P = 0.049), plasma IL-6 (r = -0.72, P < 0.001), IL-8 (r = -0.60, P = 0.002), and MCP-1 (r = -0.589, P = 0.002), but not with changes in muscle IL-8 and MCP-1 protein content, or muscle mRNA expression for IL-6, IL-8, and MCP-1.

Conclusion: Prolonged and intensive running increased muscle mRNA expression, muscle protein content, and plasma levels for IL-6, IL-8, and MCP-1, and post-run muscle glycogen levels were most strongly related to plasma cytokine levels.

No MeSH data available.


Related in: MedlinePlus