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New distribution records for the critically endangered frog Indirana gundia (Dubois, 1986) from Kerala part of Western Ghats, India.

Jesmina AS, George S - Biodivers Data J (2015)

Bottom Line: Indirana gundia is one of the critically endangered frog species of Western Ghats, India, and known only from the type locality (Gundya in Karnataka State, India) at an elevation of 200 m Mean Sea Level.We provide data on the geographical distribution of this species using molecular tools.Our results expand the geographical distribution range of this species about 111 km south up to the northern part of Kerala State and recorded at an elevation ranging from 115 m to 200 m asl.

View Article: PubMed Central - PubMed

Affiliation: Rajiv Gandhi Centre for Biotechnology, Trivandrum, India.

ABSTRACT

Background: Indirana gundia is one of the critically endangered frog species of Western Ghats, India, and known only from the type locality (Gundya in Karnataka State, India) at an elevation of 200 m Mean Sea Level. We provide data on the geographical distribution of this species using molecular tools.

New information: Our results expand the geographical distribution range of this species about 111 km south up to the northern part of Kerala State and recorded at an elevation ranging from 115 m to 200 m asl.

No MeSH data available.


Map showing the localities where I.gundia occurs in Western Ghats.
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Figure 1654464: Map showing the localities where I.gundia occurs in Western Ghats.

Mentions: Visual encounter survey Method (Heyer et al. 1994) was adopted to capture thirteen individuals from Konnakkad, three from Kanamvayal and five from Aralam under the license from the Kerala Forest Department (No. WL-12-1713; Voucher numbers are given in Fig. 2). They were released immediately in their respective habitats after collecting toe clips. Since the mitochondrial DNA sequences are available for this species, we used 16S mitochondrial gene sequences to diagnose specimens from three localities: (1) Konnakkad (12°22'1" N, 75°22'21" E) (2) Kanamvayal (12°17'41.2" N, 75°28'38.0" E) and Aralam (11°52'43.7" N, 75°53'19.0" E) in the northern part of Kerala (Fig. 1). DNA was extracted from the collected toe clips using DNeasy Animal Blood and Tissue Kit (Qiagen). The genomic DNA was amplified with 16SA-L and 16SB-H primers (Palumbi et al. 1991) for 16S ribosomal RNA fragments of the mitochondrial gene as its utility in amphibian species identification is well established (Vences et al. 2005). The thermocycling conditions for the amplification of 16S gene as follows: 95°C for 5 min followed by 40 cycles of 95°C for 30s, 55°C for 40s, 72°C for 90s followed by a final extension step at 72° for 5 min. PCR products were purified and sequenced using an ABI 3730 capillary sequencer following manufacturer's instructions. We then compared the sequences with reference sequences of I.gundia from the type locality, available from GenBank (Acc. Numbers: KM386532 and KM386533), using BLAST. To further corroborate our results, we computed genetic distance (p-distance; Suppl. material 1) and maximum likelihood (ML) phylogenetic tree using MEGA 6 (Tamura et al. 2013) under K2+I model (Kimura 1980) as the best fitting model, along with the 16S sequences of other Indirana species distributed in Kerala (Fig. 2, Suppl. material 2). The obtained sequences (494 base pair in length) were deposited in GenBank under accession numbers KT282197-KT282223.


New distribution records for the critically endangered frog Indirana gundia (Dubois, 1986) from Kerala part of Western Ghats, India.

Jesmina AS, George S - Biodivers Data J (2015)

Map showing the localities where I.gundia occurs in Western Ghats.
© Copyright Policy - creative-commons-attribution
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4563154&req=5

Figure 1654464: Map showing the localities where I.gundia occurs in Western Ghats.
Mentions: Visual encounter survey Method (Heyer et al. 1994) was adopted to capture thirteen individuals from Konnakkad, three from Kanamvayal and five from Aralam under the license from the Kerala Forest Department (No. WL-12-1713; Voucher numbers are given in Fig. 2). They were released immediately in their respective habitats after collecting toe clips. Since the mitochondrial DNA sequences are available for this species, we used 16S mitochondrial gene sequences to diagnose specimens from three localities: (1) Konnakkad (12°22'1" N, 75°22'21" E) (2) Kanamvayal (12°17'41.2" N, 75°28'38.0" E) and Aralam (11°52'43.7" N, 75°53'19.0" E) in the northern part of Kerala (Fig. 1). DNA was extracted from the collected toe clips using DNeasy Animal Blood and Tissue Kit (Qiagen). The genomic DNA was amplified with 16SA-L and 16SB-H primers (Palumbi et al. 1991) for 16S ribosomal RNA fragments of the mitochondrial gene as its utility in amphibian species identification is well established (Vences et al. 2005). The thermocycling conditions for the amplification of 16S gene as follows: 95°C for 5 min followed by 40 cycles of 95°C for 30s, 55°C for 40s, 72°C for 90s followed by a final extension step at 72° for 5 min. PCR products were purified and sequenced using an ABI 3730 capillary sequencer following manufacturer's instructions. We then compared the sequences with reference sequences of I.gundia from the type locality, available from GenBank (Acc. Numbers: KM386532 and KM386533), using BLAST. To further corroborate our results, we computed genetic distance (p-distance; Suppl. material 1) and maximum likelihood (ML) phylogenetic tree using MEGA 6 (Tamura et al. 2013) under K2+I model (Kimura 1980) as the best fitting model, along with the 16S sequences of other Indirana species distributed in Kerala (Fig. 2, Suppl. material 2). The obtained sequences (494 base pair in length) were deposited in GenBank under accession numbers KT282197-KT282223.

Bottom Line: Indirana gundia is one of the critically endangered frog species of Western Ghats, India, and known only from the type locality (Gundya in Karnataka State, India) at an elevation of 200 m Mean Sea Level.We provide data on the geographical distribution of this species using molecular tools.Our results expand the geographical distribution range of this species about 111 km south up to the northern part of Kerala State and recorded at an elevation ranging from 115 m to 200 m asl.

View Article: PubMed Central - PubMed

Affiliation: Rajiv Gandhi Centre for Biotechnology, Trivandrum, India.

ABSTRACT

Background: Indirana gundia is one of the critically endangered frog species of Western Ghats, India, and known only from the type locality (Gundya in Karnataka State, India) at an elevation of 200 m Mean Sea Level. We provide data on the geographical distribution of this species using molecular tools.

New information: Our results expand the geographical distribution range of this species about 111 km south up to the northern part of Kerala State and recorded at an elevation ranging from 115 m to 200 m asl.

No MeSH data available.