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Telomere G-tail Length is a Promising Biomarker Related to White Matter Lesions and Endothelial Dysfunction in Patients With Cardiovascular Risk: A Cross-sectional Study.

Nezu T, Hosomi N, Takahashi T, Anno K, Aoki S, Shimamoto A, Maruyama H, Hayashi T, Matsumoto M, Tahara H - EBioMedicine (2015)

Bottom Line: Shortened telomere G-tail length was associated with age and Framingham risk score (P = 0.018 and P = 0.012).On multivariate regression analysis, telomere G-tail length was independently associated with FMD values (P = 0.022) and the severity of ARWMCs (P = 0.033), whereas total telomere length was not associated with these indicators.Telomere G-tail length is associated with age and vascular risk factors, and might be superior to total telomere length as a marker of endothelial dysfunction and ARWMC severity.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan.

ABSTRACT

Background: The telomeric 3'-overhang (G-tail) length is essential for the biological effects of telomere dysfunction in vitro, but the association of length with aging and cardiovascular risk is unclear in humans. We investigated the association between the telomere G-tail length of leukocytes and cardiovascular risk, age-related white matter changes (ARWMCs), and endothelial function.

Methods: Patients with a history of cerebrovascular disease and comorbidity were enrolled (n = 102; 69 males and 33 females, 70.1 ± 9.2 years). Total telomere and telomere G-tail lengths were measured using a hybridization protection assay. Endothelial function was evaluated by ultrasound assessment of brachial flow-mediated dilation (FMD).

Findings: Shortened telomere G-tail length was associated with age and Framingham risk score (P = 0.018 and P = 0.012). In addition, telomere G-tail length was positively correlated with FMD values (P = 0.031) and negatively with the severity of ARWMCs (P = 0.002). On multivariate regression analysis, telomere G-tail length was independently associated with FMD values (P = 0.022) and the severity of ARWMCs (P = 0.033), whereas total telomere length was not associated with these indicators.

Interpretation: Telomere G-tail length is associated with age and vascular risk factors, and might be superior to total telomere length as a marker of endothelial dysfunction and ARWMC severity.

No MeSH data available.


Related in: MedlinePlus

(A) Scatter plots demonstrating the relationship between telomere G-tail length and flow-mediated dilation (FMD). (B) Scatter plots demonstrating the relationship between total telomere length and FMD. (C) Scatter plots demonstrating the relationship between telomere G-tail length and age-related white matter changes (ARWMCs). (D) Scatter plots demonstrating the relationship between total telomere length and ARWMCs.
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f0020: (A) Scatter plots demonstrating the relationship between telomere G-tail length and flow-mediated dilation (FMD). (B) Scatter plots demonstrating the relationship between total telomere length and FMD. (C) Scatter plots demonstrating the relationship between telomere G-tail length and age-related white matter changes (ARWMCs). (D) Scatter plots demonstrating the relationship between total telomere length and ARWMCs.

Mentions: On univariate regression analysis, FMD was associated with age (ρ − 0.266, P = 0.007), male sex (ρ − 0.309, P = 0.002), BMI (ρ − 0.255, P = 0.010), hypertension (ρ − 0.305, P = 0.002), diabetes mellitus (ρ − 0.277, P = 0.005), renal dysfunction (ρ − 0.208, P = 0.036), high-density lipoprotein cholesterol level (ρ 0.254, P = 0.010), triglyceride level (ρ − 0.280, P = 0.004), eGFR (ρ 0.199, P = 0.045) and hs-CRP levels (ρ − 0.224, P = 0.024) (Table 3). Telomere G-tail length was positively correlated with FMD values (ρ 0.214, P = 0.031), whereas total telomere length was not significantly associated with FMD (ρ 0.112, P = 0.263) (Fig. 3A, B). Longer telomere G-tail length was independently associated with FMD value (standardized partial regression coefficient [β] 0.205; P = 0.022) on multivariate regression analysis for age, sex, comorbidity and other laboratory findings (Table 3). In contrast, total telomere length was not associated with FMD value on multivariate regression analysis using the backward selection procedure. In addition, total telomere length was not associated with FMD value after adjustment for age and sex (β 0.056; P = 0.574).


Telomere G-tail Length is a Promising Biomarker Related to White Matter Lesions and Endothelial Dysfunction in Patients With Cardiovascular Risk: A Cross-sectional Study.

Nezu T, Hosomi N, Takahashi T, Anno K, Aoki S, Shimamoto A, Maruyama H, Hayashi T, Matsumoto M, Tahara H - EBioMedicine (2015)

(A) Scatter plots demonstrating the relationship between telomere G-tail length and flow-mediated dilation (FMD). (B) Scatter plots demonstrating the relationship between total telomere length and FMD. (C) Scatter plots demonstrating the relationship between telomere G-tail length and age-related white matter changes (ARWMCs). (D) Scatter plots demonstrating the relationship between total telomere length and ARWMCs.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4563121&req=5

f0020: (A) Scatter plots demonstrating the relationship between telomere G-tail length and flow-mediated dilation (FMD). (B) Scatter plots demonstrating the relationship between total telomere length and FMD. (C) Scatter plots demonstrating the relationship between telomere G-tail length and age-related white matter changes (ARWMCs). (D) Scatter plots demonstrating the relationship between total telomere length and ARWMCs.
Mentions: On univariate regression analysis, FMD was associated with age (ρ − 0.266, P = 0.007), male sex (ρ − 0.309, P = 0.002), BMI (ρ − 0.255, P = 0.010), hypertension (ρ − 0.305, P = 0.002), diabetes mellitus (ρ − 0.277, P = 0.005), renal dysfunction (ρ − 0.208, P = 0.036), high-density lipoprotein cholesterol level (ρ 0.254, P = 0.010), triglyceride level (ρ − 0.280, P = 0.004), eGFR (ρ 0.199, P = 0.045) and hs-CRP levels (ρ − 0.224, P = 0.024) (Table 3). Telomere G-tail length was positively correlated with FMD values (ρ 0.214, P = 0.031), whereas total telomere length was not significantly associated with FMD (ρ 0.112, P = 0.263) (Fig. 3A, B). Longer telomere G-tail length was independently associated with FMD value (standardized partial regression coefficient [β] 0.205; P = 0.022) on multivariate regression analysis for age, sex, comorbidity and other laboratory findings (Table 3). In contrast, total telomere length was not associated with FMD value on multivariate regression analysis using the backward selection procedure. In addition, total telomere length was not associated with FMD value after adjustment for age and sex (β 0.056; P = 0.574).

Bottom Line: Shortened telomere G-tail length was associated with age and Framingham risk score (P = 0.018 and P = 0.012).On multivariate regression analysis, telomere G-tail length was independently associated with FMD values (P = 0.022) and the severity of ARWMCs (P = 0.033), whereas total telomere length was not associated with these indicators.Telomere G-tail length is associated with age and vascular risk factors, and might be superior to total telomere length as a marker of endothelial dysfunction and ARWMC severity.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan.

ABSTRACT

Background: The telomeric 3'-overhang (G-tail) length is essential for the biological effects of telomere dysfunction in vitro, but the association of length with aging and cardiovascular risk is unclear in humans. We investigated the association between the telomere G-tail length of leukocytes and cardiovascular risk, age-related white matter changes (ARWMCs), and endothelial function.

Methods: Patients with a history of cerebrovascular disease and comorbidity were enrolled (n = 102; 69 males and 33 females, 70.1 ± 9.2 years). Total telomere and telomere G-tail lengths were measured using a hybridization protection assay. Endothelial function was evaluated by ultrasound assessment of brachial flow-mediated dilation (FMD).

Findings: Shortened telomere G-tail length was associated with age and Framingham risk score (P = 0.018 and P = 0.012). In addition, telomere G-tail length was positively correlated with FMD values (P = 0.031) and negatively with the severity of ARWMCs (P = 0.002). On multivariate regression analysis, telomere G-tail length was independently associated with FMD values (P = 0.022) and the severity of ARWMCs (P = 0.033), whereas total telomere length was not associated with these indicators.

Interpretation: Telomere G-tail length is associated with age and vascular risk factors, and might be superior to total telomere length as a marker of endothelial dysfunction and ARWMC severity.

No MeSH data available.


Related in: MedlinePlus