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Telomere G-tail Length is a Promising Biomarker Related to White Matter Lesions and Endothelial Dysfunction in Patients With Cardiovascular Risk: A Cross-sectional Study.

Nezu T, Hosomi N, Takahashi T, Anno K, Aoki S, Shimamoto A, Maruyama H, Hayashi T, Matsumoto M, Tahara H - EBioMedicine (2015)

Bottom Line: Shortened telomere G-tail length was associated with age and Framingham risk score (P = 0.018 and P = 0.012).On multivariate regression analysis, telomere G-tail length was independently associated with FMD values (P = 0.022) and the severity of ARWMCs (P = 0.033), whereas total telomere length was not associated with these indicators.Telomere G-tail length is associated with age and vascular risk factors, and might be superior to total telomere length as a marker of endothelial dysfunction and ARWMC severity.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan.

ABSTRACT

Background: The telomeric 3'-overhang (G-tail) length is essential for the biological effects of telomere dysfunction in vitro, but the association of length with aging and cardiovascular risk is unclear in humans. We investigated the association between the telomere G-tail length of leukocytes and cardiovascular risk, age-related white matter changes (ARWMCs), and endothelial function.

Methods: Patients with a history of cerebrovascular disease and comorbidity were enrolled (n = 102; 69 males and 33 females, 70.1 ± 9.2 years). Total telomere and telomere G-tail lengths were measured using a hybridization protection assay. Endothelial function was evaluated by ultrasound assessment of brachial flow-mediated dilation (FMD).

Findings: Shortened telomere G-tail length was associated with age and Framingham risk score (P = 0.018 and P = 0.012). In addition, telomere G-tail length was positively correlated with FMD values (P = 0.031) and negatively with the severity of ARWMCs (P = 0.002). On multivariate regression analysis, telomere G-tail length was independently associated with FMD values (P = 0.022) and the severity of ARWMCs (P = 0.033), whereas total telomere length was not associated with these indicators.

Interpretation: Telomere G-tail length is associated with age and vascular risk factors, and might be superior to total telomere length as a marker of endothelial dysfunction and ARWMC severity.

No MeSH data available.


Related in: MedlinePlus

(A) Scatter plots representing the relationship between telomere G-tail length and age in patients and control subjects. (B) Scatter plots demonstrating the relationship between telomere G-tail length and total telomere lengths in patients and control subjects.
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f0010: (A) Scatter plots representing the relationship between telomere G-tail length and age in patients and control subjects. (B) Scatter plots demonstrating the relationship between telomere G-tail length and total telomere lengths in patients and control subjects.

Mentions: A total of 102 patients (69 males and 33 females, 70.1 ± 9.2 years) were registered in the study. Baseline clinical characteristics are presented in Table 1. Telomere G-tail length was negatively correlated with aging and positively correlated with total telomere length (ρ − 0.287, P = 0.004 and ρ 0.406, P < 0.001, Fig. 1). Neither telomere G-tail length nor total telomere length was associated with laboratory findings, including altered glucose levels, lipid levels, renal dysfunction or inflammation (Supplemental Table 1). Patients in this study had a shorter mean telomere G-tail length than control subjects (13653.0 ± 2787.4 RLU/μg DNA vs. 22504.9 ± 3249.1 RLU/μg DNA, P < 0.001), but did not significantly differ by total telomere length (Supplemental Table 2). Further, the groups significantly differed by distribution of the association between age and telomere G-tail length (Fig. 1A), and also significantly differed in the distribution of associations with total telomere length and telomere G-tail length (Fig. 1B).


Telomere G-tail Length is a Promising Biomarker Related to White Matter Lesions and Endothelial Dysfunction in Patients With Cardiovascular Risk: A Cross-sectional Study.

Nezu T, Hosomi N, Takahashi T, Anno K, Aoki S, Shimamoto A, Maruyama H, Hayashi T, Matsumoto M, Tahara H - EBioMedicine (2015)

(A) Scatter plots representing the relationship between telomere G-tail length and age in patients and control subjects. (B) Scatter plots demonstrating the relationship between telomere G-tail length and total telomere lengths in patients and control subjects.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4563121&req=5

f0010: (A) Scatter plots representing the relationship between telomere G-tail length and age in patients and control subjects. (B) Scatter plots demonstrating the relationship between telomere G-tail length and total telomere lengths in patients and control subjects.
Mentions: A total of 102 patients (69 males and 33 females, 70.1 ± 9.2 years) were registered in the study. Baseline clinical characteristics are presented in Table 1. Telomere G-tail length was negatively correlated with aging and positively correlated with total telomere length (ρ − 0.287, P = 0.004 and ρ 0.406, P < 0.001, Fig. 1). Neither telomere G-tail length nor total telomere length was associated with laboratory findings, including altered glucose levels, lipid levels, renal dysfunction or inflammation (Supplemental Table 1). Patients in this study had a shorter mean telomere G-tail length than control subjects (13653.0 ± 2787.4 RLU/μg DNA vs. 22504.9 ± 3249.1 RLU/μg DNA, P < 0.001), but did not significantly differ by total telomere length (Supplemental Table 2). Further, the groups significantly differed by distribution of the association between age and telomere G-tail length (Fig. 1A), and also significantly differed in the distribution of associations with total telomere length and telomere G-tail length (Fig. 1B).

Bottom Line: Shortened telomere G-tail length was associated with age and Framingham risk score (P = 0.018 and P = 0.012).On multivariate regression analysis, telomere G-tail length was independently associated with FMD values (P = 0.022) and the severity of ARWMCs (P = 0.033), whereas total telomere length was not associated with these indicators.Telomere G-tail length is associated with age and vascular risk factors, and might be superior to total telomere length as a marker of endothelial dysfunction and ARWMC severity.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan.

ABSTRACT

Background: The telomeric 3'-overhang (G-tail) length is essential for the biological effects of telomere dysfunction in vitro, but the association of length with aging and cardiovascular risk is unclear in humans. We investigated the association between the telomere G-tail length of leukocytes and cardiovascular risk, age-related white matter changes (ARWMCs), and endothelial function.

Methods: Patients with a history of cerebrovascular disease and comorbidity were enrolled (n = 102; 69 males and 33 females, 70.1 ± 9.2 years). Total telomere and telomere G-tail lengths were measured using a hybridization protection assay. Endothelial function was evaluated by ultrasound assessment of brachial flow-mediated dilation (FMD).

Findings: Shortened telomere G-tail length was associated with age and Framingham risk score (P = 0.018 and P = 0.012). In addition, telomere G-tail length was positively correlated with FMD values (P = 0.031) and negatively with the severity of ARWMCs (P = 0.002). On multivariate regression analysis, telomere G-tail length was independently associated with FMD values (P = 0.022) and the severity of ARWMCs (P = 0.033), whereas total telomere length was not associated with these indicators.

Interpretation: Telomere G-tail length is associated with age and vascular risk factors, and might be superior to total telomere length as a marker of endothelial dysfunction and ARWMC severity.

No MeSH data available.


Related in: MedlinePlus