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Biothermodynamic Assay of Coptis-Evodia Herb Couples.

Yang H, Su M, Yao Q, Zhao Y, Chen D, Jia L - Evid Based Complement Alternat Med (2015)

Bottom Line: It showed that animal retention ratio in hot pad significantly decreased along with the decrease in coptis proportion in coptis-evodia herb couples.In addition, Zuojin wan markedly reduced the retention ratio of gastritis mice in the hot pad, while Fanzuojin wan displayed an opposite result.In the gastric cells from the gastritis mice, Fanzuojin wan remarkably increased calorific value for growth and metabolism, while Zuojin wan significantly reduced the calorigenic effect.

View Article: PubMed Central - PubMed

Affiliation: Yunnan Institute of Materia Medica, Kunming 650111, China.

ABSTRACT
Objective. To illustrate the difference in cold/hot natural properties and therapeutic effect of coptis-evodia herb couples by using cold/hot plate differentiating technology and microcalorimetry combined with material basis analysis in vivo and in vitro. It showed that animal retention ratio in hot pad significantly decreased along with the decrease in coptis proportion in coptis-evodia herb couples. In addition, Zuojin wan markedly reduced the retention ratio of gastritis mice in the hot pad, while Fanzuojin wan displayed an opposite result. Further, Mg(2+)-ATPase, Ca(2+)-ATPase, and T-AOC activity significantly weakened in coptis-treated group in the livers of the mice. In the gastric cells from the gastritis mice, Fanzuojin wan remarkably increased calorific value for growth and metabolism, while Zuojin wan significantly reduced the calorigenic effect. It suggested that the changes in the major chemical compositions (especially alkaloids) were the material base-induced transformation between "cold" and "hot" syndromes. The material basis which affected the transformation between "cold" and "hot" syndromes might be X2, X3, X4, X8, epiberberine hydrochloride, jatrorrhizine hydrochloride, coptisine sulphate, palmatine hydrochloride, and berberine hydrochloride. The CHPD combined with microcalorimetry technology is a good method to determine the differences in the "cold" and "hot" natural properties of coptis-evodia herb couples.

No MeSH data available.


Related in: MedlinePlus

Chromatogram of coptis, evodia, and mixed standard solution. (a): coptis; (b): evodia; (c): mixed standard solution. 11—hydroevodiamine; 15—epiberberine; 16—jatrorrhizine; 17—coptisine; 19—palmatine; 20—berberine; 22—evodiamine; 23—rutaecarpine; 1–10, 12–14, 18, and 21—other unknown materials.
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fig10: Chromatogram of coptis, evodia, and mixed standard solution. (a): coptis; (b): evodia; (c): mixed standard solution. 11—hydroevodiamine; 15—epiberberine; 16—jatrorrhizine; 17—coptisine; 19—palmatine; 20—berberine; 22—evodiamine; 23—rutaecarpine; 1–10, 12–14, 18, and 21—other unknown materials.

Mentions: For the area of berberine hydrochloride peak (wave 20#) was largest in the total area (10% or more) with the highest peak height and relative stability, it was selected for the control wave. Under the above chromatographic conditions, 1 μL of coptis-evodia herb couples solutions was injected to record the chromatograms (Figure 10).


Biothermodynamic Assay of Coptis-Evodia Herb Couples.

Yang H, Su M, Yao Q, Zhao Y, Chen D, Jia L - Evid Based Complement Alternat Med (2015)

Chromatogram of coptis, evodia, and mixed standard solution. (a): coptis; (b): evodia; (c): mixed standard solution. 11—hydroevodiamine; 15—epiberberine; 16—jatrorrhizine; 17—coptisine; 19—palmatine; 20—berberine; 22—evodiamine; 23—rutaecarpine; 1–10, 12–14, 18, and 21—other unknown materials.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4563112&req=5

fig10: Chromatogram of coptis, evodia, and mixed standard solution. (a): coptis; (b): evodia; (c): mixed standard solution. 11—hydroevodiamine; 15—epiberberine; 16—jatrorrhizine; 17—coptisine; 19—palmatine; 20—berberine; 22—evodiamine; 23—rutaecarpine; 1–10, 12–14, 18, and 21—other unknown materials.
Mentions: For the area of berberine hydrochloride peak (wave 20#) was largest in the total area (10% or more) with the highest peak height and relative stability, it was selected for the control wave. Under the above chromatographic conditions, 1 μL of coptis-evodia herb couples solutions was injected to record the chromatograms (Figure 10).

Bottom Line: It showed that animal retention ratio in hot pad significantly decreased along with the decrease in coptis proportion in coptis-evodia herb couples.In addition, Zuojin wan markedly reduced the retention ratio of gastritis mice in the hot pad, while Fanzuojin wan displayed an opposite result.In the gastric cells from the gastritis mice, Fanzuojin wan remarkably increased calorific value for growth and metabolism, while Zuojin wan significantly reduced the calorigenic effect.

View Article: PubMed Central - PubMed

Affiliation: Yunnan Institute of Materia Medica, Kunming 650111, China.

ABSTRACT
Objective. To illustrate the difference in cold/hot natural properties and therapeutic effect of coptis-evodia herb couples by using cold/hot plate differentiating technology and microcalorimetry combined with material basis analysis in vivo and in vitro. It showed that animal retention ratio in hot pad significantly decreased along with the decrease in coptis proportion in coptis-evodia herb couples. In addition, Zuojin wan markedly reduced the retention ratio of gastritis mice in the hot pad, while Fanzuojin wan displayed an opposite result. Further, Mg(2+)-ATPase, Ca(2+)-ATPase, and T-AOC activity significantly weakened in coptis-treated group in the livers of the mice. In the gastric cells from the gastritis mice, Fanzuojin wan remarkably increased calorific value for growth and metabolism, while Zuojin wan significantly reduced the calorigenic effect. It suggested that the changes in the major chemical compositions (especially alkaloids) were the material base-induced transformation between "cold" and "hot" syndromes. The material basis which affected the transformation between "cold" and "hot" syndromes might be X2, X3, X4, X8, epiberberine hydrochloride, jatrorrhizine hydrochloride, coptisine sulphate, palmatine hydrochloride, and berberine hydrochloride. The CHPD combined with microcalorimetry technology is a good method to determine the differences in the "cold" and "hot" natural properties of coptis-evodia herb couples.

No MeSH data available.


Related in: MedlinePlus