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Genotype-Dependent Difference in 5-HT2C Receptor-Induced Hypolocomotion: Comparison with 5-HT2A Receptor Functional Activity.

Bazovkina DV, Kondaurova EM, Naumenko VS, Ponimaskin E - Neural Plast. (2015)

Bottom Line: This effect was receptor-specific because it was inhibited by the 5-HT2C receptor antagonist RS102221.We also compared the interstrain differences in functional response to 5-HT2C and 5-HT2A receptors activation measured by the quantification of receptor-mediated head-twitches.Taken together, our data indicate the implication of 5-HT2C receptors in regulation of locomotor activity and suggest the shared mechanism for functional responses mediated by 5-HT2C and 5-HT2A receptors.

View Article: PubMed Central - PubMed

Affiliation: Institute of Cytology and Genetics, Siberian Division of Russian Academy of Sciences, Novosibirsk 630090, Russia.

ABSTRACT
In the present study behavioral effects of the 5-HT2C serotonin receptor were investigated in different mouse strains. The 5-HT2C receptor agonist MK-212 applied intraperitoneally induced significant dose-dependent reduction of distance traveled in the open field test in CBA/Lac mice. This effect was receptor-specific because it was inhibited by the 5-HT2C receptor antagonist RS102221. To study the role of genotype in 5-HT2C receptor-induced hypolocomotion, locomotor activity of seven inbred mouse strains was measured after MK-212 acute treatment. We found that the 5-HT2C receptor stimulation by MK-212 decreased distance traveled in the open field test in CBA/Lac, C57Bl/6, C3H/He, and ICR mice, whereas it failed to affect locomotor activity in DBA/2J, Asn, and Balb/c mice. We also compared the interstrain differences in functional response to 5-HT2C and 5-HT2A receptors activation measured by the quantification of receptor-mediated head-twitches. These experiments revealed significant positive correlation between 5-HT2C and 5-HT2A receptors functional responses for all investigated mouse strains. Moreover, we found that 5-HT2A receptor activation with DOI did not change locomotor activity in CBA/Lac mice. Taken together, our data indicate the implication of 5-HT2C receptors in regulation of locomotor activity and suggest the shared mechanism for functional responses mediated by 5-HT2C and 5-HT2A receptors.

No MeSH data available.


Related in: MedlinePlus

Effect of selective 5-HT2C receptor agonist MK-212 (2 mg/kg, i.p.) on locomotor activity in the open field test in seven inbred mouse strains. The data are presented as mean ± SEM (n = 8 per group; ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with corresponding control).
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fig3: Effect of selective 5-HT2C receptor agonist MK-212 (2 mg/kg, i.p.) on locomotor activity in the open field test in seven inbred mouse strains. The data are presented as mean ± SEM (n = 8 per group; ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with corresponding control).

Mentions: To study the role of genotype in behavioral effect mediated by the 5-HT2C receptor, mice of seven inbred strains were tested in the open field test after acute treatment with MK-212 (i.p., 2.0 mg/kg; Figure 3). We have chosen the 2.0 mg/kg dose of MK-212 because it produced considerable, but not dramatic, decrease in distance traveled in the open field. In these experiments we obtained significant effect of the drug (F1,93 = 16.3, p < 0.001), genotype (F6,93 = 12.3, p < 0.001), and genotype × drug interaction (F6,93 = 3.0, p < 0.05) on distance traveled. It is however noteworthy that MK-212 decreased locomotor activity in the open field test only in CBA/Lac, C57Bl/6, C3H/He, and ICR strains with the most significant effect obtained in CBA/Lac and C3H/He mouse strains, in which MK-212 led to more than twofold reduction of locomotor activity (Figure 3). In contrast, the 5-HT2C receptor stimulation did not alter the distance traveled in DBA/2J, Asn, and Balb/c strains (Figure 3). The decrease of distance traveled in the open field test was about 30 percent in C57Bl/6 and ICR animals treated with MK-212, when compared with corresponding saline groups (Figure 4(a)). Thus, 5-HT2C receptor-mediated hypolocomotion is strongly dependent on genotype.


Genotype-Dependent Difference in 5-HT2C Receptor-Induced Hypolocomotion: Comparison with 5-HT2A Receptor Functional Activity.

Bazovkina DV, Kondaurova EM, Naumenko VS, Ponimaskin E - Neural Plast. (2015)

Effect of selective 5-HT2C receptor agonist MK-212 (2 mg/kg, i.p.) on locomotor activity in the open field test in seven inbred mouse strains. The data are presented as mean ± SEM (n = 8 per group; ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with corresponding control).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4563107&req=5

fig3: Effect of selective 5-HT2C receptor agonist MK-212 (2 mg/kg, i.p.) on locomotor activity in the open field test in seven inbred mouse strains. The data are presented as mean ± SEM (n = 8 per group; ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with corresponding control).
Mentions: To study the role of genotype in behavioral effect mediated by the 5-HT2C receptor, mice of seven inbred strains were tested in the open field test after acute treatment with MK-212 (i.p., 2.0 mg/kg; Figure 3). We have chosen the 2.0 mg/kg dose of MK-212 because it produced considerable, but not dramatic, decrease in distance traveled in the open field. In these experiments we obtained significant effect of the drug (F1,93 = 16.3, p < 0.001), genotype (F6,93 = 12.3, p < 0.001), and genotype × drug interaction (F6,93 = 3.0, p < 0.05) on distance traveled. It is however noteworthy that MK-212 decreased locomotor activity in the open field test only in CBA/Lac, C57Bl/6, C3H/He, and ICR strains with the most significant effect obtained in CBA/Lac and C3H/He mouse strains, in which MK-212 led to more than twofold reduction of locomotor activity (Figure 3). In contrast, the 5-HT2C receptor stimulation did not alter the distance traveled in DBA/2J, Asn, and Balb/c strains (Figure 3). The decrease of distance traveled in the open field test was about 30 percent in C57Bl/6 and ICR animals treated with MK-212, when compared with corresponding saline groups (Figure 4(a)). Thus, 5-HT2C receptor-mediated hypolocomotion is strongly dependent on genotype.

Bottom Line: This effect was receptor-specific because it was inhibited by the 5-HT2C receptor antagonist RS102221.We also compared the interstrain differences in functional response to 5-HT2C and 5-HT2A receptors activation measured by the quantification of receptor-mediated head-twitches.Taken together, our data indicate the implication of 5-HT2C receptors in regulation of locomotor activity and suggest the shared mechanism for functional responses mediated by 5-HT2C and 5-HT2A receptors.

View Article: PubMed Central - PubMed

Affiliation: Institute of Cytology and Genetics, Siberian Division of Russian Academy of Sciences, Novosibirsk 630090, Russia.

ABSTRACT
In the present study behavioral effects of the 5-HT2C serotonin receptor were investigated in different mouse strains. The 5-HT2C receptor agonist MK-212 applied intraperitoneally induced significant dose-dependent reduction of distance traveled in the open field test in CBA/Lac mice. This effect was receptor-specific because it was inhibited by the 5-HT2C receptor antagonist RS102221. To study the role of genotype in 5-HT2C receptor-induced hypolocomotion, locomotor activity of seven inbred mouse strains was measured after MK-212 acute treatment. We found that the 5-HT2C receptor stimulation by MK-212 decreased distance traveled in the open field test in CBA/Lac, C57Bl/6, C3H/He, and ICR mice, whereas it failed to affect locomotor activity in DBA/2J, Asn, and Balb/c mice. We also compared the interstrain differences in functional response to 5-HT2C and 5-HT2A receptors activation measured by the quantification of receptor-mediated head-twitches. These experiments revealed significant positive correlation between 5-HT2C and 5-HT2A receptors functional responses for all investigated mouse strains. Moreover, we found that 5-HT2A receptor activation with DOI did not change locomotor activity in CBA/Lac mice. Taken together, our data indicate the implication of 5-HT2C receptors in regulation of locomotor activity and suggest the shared mechanism for functional responses mediated by 5-HT2C and 5-HT2A receptors.

No MeSH data available.


Related in: MedlinePlus