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Protective effects of Carissa opaca fruits against CCl4-induced oxidative kidney lipid peroxidation and trauma in rat.

Sahreen S, Khan MR, Khan RA, Alkreathy HM - Food Nutr Res (2015)

Bottom Line: The protective effects of various fractions of C. opaca fruits against CCl4 administration were reviewed by rat renal function alterations.Chronic toxicity caused by 8-week treatment of CCl4 to the rats significantly decreased the pH level, activities of antioxidant enzymes, and glutathione contents, whereas a significant increase was found in the case of specific gravity, red blood cells, white blood cells, level of urea, and lipid peroxidation in comparison to control group.Results revealed that the methanolic fractions of C. opaca are the most potent and helpful in kidney trauma.

View Article: PubMed Central - PubMed

Affiliation: Botanical Sciences Division, Pakistan Museum of Natural History, Islamabad, Pakistan.

ABSTRACT

Background: Carbon tetrachloride (CCl4) is a potent nephrotoxin, as it causes acute as well as chronic toxicity in kidneys. Therefore, this study was carried out to assess the pharmacological potential of different fractions of Carissa opaca fruits on CCl4-induced oxidative trauma in the kidney.

Methods: The parameters studied in this respect were the kidney function tests viz, serum profile, urine profile, genotoxicity, characteristic morphological findings, and antioxidant enzymatic level of kidneys.

Result: The protective effects of various fractions of C. opaca fruits against CCl4 administration were reviewed by rat renal function alterations. Chronic toxicity caused by 8-week treatment of CCl4 to the rats significantly decreased the pH level, activities of antioxidant enzymes, and glutathione contents, whereas a significant increase was found in the case of specific gravity, red blood cells, white blood cells, level of urea, and lipid peroxidation in comparison to control group. Administration of various fractions of C. opaca fruit with CCl4 showed protective ability against CCl4 intoxication by restoring the urine profile, activities of antioxidant enzymes, and lipid peroxidation in rat. CCl4 induction in rats also caused DNA fragmentation and glomerular atrophy by means of dilation, disappearance of Bowmen's space, congestion in the capillary loops, dilation in renal tubules, and foamy look of epithelial cells of tubular region, which were restored by co-admiration of various fractions of C. opaca.

Conclusion: Results revealed that the methanolic fractions of C. opaca are the most potent and helpful in kidney trauma.

No MeSH data available.


Related in: MedlinePlus

Microphotograph of rat kidney (hematoxylin and eosin stain) (a). Representative section of renal from the control group showing normal histology, (b) DMSO+Olive oil group, (c) CCl4 group, (d) silymarin+CCl4 group, (e) MFC+CCl4 group, (f) EFC+CCl4 group, (g) HFC+CCl4 group, () glomerular atrophy, () vascular congestions around the tubules, () alterations in Bowmen's space, (▲) tubular dilations, () distorted renal corpuscles.
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Figure 0002: Microphotograph of rat kidney (hematoxylin and eosin stain) (a). Representative section of renal from the control group showing normal histology, (b) DMSO+Olive oil group, (c) CCl4 group, (d) silymarin+CCl4 group, (e) MFC+CCl4 group, (f) EFC+CCl4 group, (g) HFC+CCl4 group, () glomerular atrophy, () vascular congestions around the tubules, () alterations in Bowmen's space, (▲) tubular dilations, () distorted renal corpuscles.

Mentions: Histoarchitecture of kidneys of different groups is summarized in Fig. 2. The histopathologically observed changes showed that the intraperitoneal injection of CCl4 for 8 weeks resulted in severe impairment to corticular region of kidneys. The damage induced was in different forms of degenerations showing glomerular atrophy by means of dilation and disappearance of Bowmen's space, congestion in the capillary loops, dilation in renal tubules, and foamy look of epithelial cells of tubular region (Fig. 2c). In addition, subchronic administration of CCl4 exhibited interstitial inflammatory cells infiltration in both corticular and medullary regions. The evaluation of kidney section of control and DMSO group had normal histological architecture as shown in Fig. 2a and b. Renal sections of rats treated with various fractions of C. opaca fruits (HFC, EFC, and MFC) reversed the CCl4 intoxication and showed mild injury in contrast to control group (Fig. 2d–f). The histological appearance of glomeruli and Bowman's capsule was almost normal, and only some of the glomeruli were degenerated. Mild necrosis in the glomeruli and tubules was observed. As a measure of protective effects, silymarin was also induced to a group of rats (Fig. 2d). Silymarin treatment erased the CCl4 pathogenesis, relative to control group. These findings are in agreement with the rest of the parameters studied.


Protective effects of Carissa opaca fruits against CCl4-induced oxidative kidney lipid peroxidation and trauma in rat.

Sahreen S, Khan MR, Khan RA, Alkreathy HM - Food Nutr Res (2015)

Microphotograph of rat kidney (hematoxylin and eosin stain) (a). Representative section of renal from the control group showing normal histology, (b) DMSO+Olive oil group, (c) CCl4 group, (d) silymarin+CCl4 group, (e) MFC+CCl4 group, (f) EFC+CCl4 group, (g) HFC+CCl4 group, () glomerular atrophy, () vascular congestions around the tubules, () alterations in Bowmen's space, (▲) tubular dilations, () distorted renal corpuscles.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4563101&req=5

Figure 0002: Microphotograph of rat kidney (hematoxylin and eosin stain) (a). Representative section of renal from the control group showing normal histology, (b) DMSO+Olive oil group, (c) CCl4 group, (d) silymarin+CCl4 group, (e) MFC+CCl4 group, (f) EFC+CCl4 group, (g) HFC+CCl4 group, () glomerular atrophy, () vascular congestions around the tubules, () alterations in Bowmen's space, (▲) tubular dilations, () distorted renal corpuscles.
Mentions: Histoarchitecture of kidneys of different groups is summarized in Fig. 2. The histopathologically observed changes showed that the intraperitoneal injection of CCl4 for 8 weeks resulted in severe impairment to corticular region of kidneys. The damage induced was in different forms of degenerations showing glomerular atrophy by means of dilation and disappearance of Bowmen's space, congestion in the capillary loops, dilation in renal tubules, and foamy look of epithelial cells of tubular region (Fig. 2c). In addition, subchronic administration of CCl4 exhibited interstitial inflammatory cells infiltration in both corticular and medullary regions. The evaluation of kidney section of control and DMSO group had normal histological architecture as shown in Fig. 2a and b. Renal sections of rats treated with various fractions of C. opaca fruits (HFC, EFC, and MFC) reversed the CCl4 intoxication and showed mild injury in contrast to control group (Fig. 2d–f). The histological appearance of glomeruli and Bowman's capsule was almost normal, and only some of the glomeruli were degenerated. Mild necrosis in the glomeruli and tubules was observed. As a measure of protective effects, silymarin was also induced to a group of rats (Fig. 2d). Silymarin treatment erased the CCl4 pathogenesis, relative to control group. These findings are in agreement with the rest of the parameters studied.

Bottom Line: The protective effects of various fractions of C. opaca fruits against CCl4 administration were reviewed by rat renal function alterations.Chronic toxicity caused by 8-week treatment of CCl4 to the rats significantly decreased the pH level, activities of antioxidant enzymes, and glutathione contents, whereas a significant increase was found in the case of specific gravity, red blood cells, white blood cells, level of urea, and lipid peroxidation in comparison to control group.Results revealed that the methanolic fractions of C. opaca are the most potent and helpful in kidney trauma.

View Article: PubMed Central - PubMed

Affiliation: Botanical Sciences Division, Pakistan Museum of Natural History, Islamabad, Pakistan.

ABSTRACT

Background: Carbon tetrachloride (CCl4) is a potent nephrotoxin, as it causes acute as well as chronic toxicity in kidneys. Therefore, this study was carried out to assess the pharmacological potential of different fractions of Carissa opaca fruits on CCl4-induced oxidative trauma in the kidney.

Methods: The parameters studied in this respect were the kidney function tests viz, serum profile, urine profile, genotoxicity, characteristic morphological findings, and antioxidant enzymatic level of kidneys.

Result: The protective effects of various fractions of C. opaca fruits against CCl4 administration were reviewed by rat renal function alterations. Chronic toxicity caused by 8-week treatment of CCl4 to the rats significantly decreased the pH level, activities of antioxidant enzymes, and glutathione contents, whereas a significant increase was found in the case of specific gravity, red blood cells, white blood cells, level of urea, and lipid peroxidation in comparison to control group. Administration of various fractions of C. opaca fruit with CCl4 showed protective ability against CCl4 intoxication by restoring the urine profile, activities of antioxidant enzymes, and lipid peroxidation in rat. CCl4 induction in rats also caused DNA fragmentation and glomerular atrophy by means of dilation, disappearance of Bowmen's space, congestion in the capillary loops, dilation in renal tubules, and foamy look of epithelial cells of tubular region, which were restored by co-admiration of various fractions of C. opaca.

Conclusion: Results revealed that the methanolic fractions of C. opaca are the most potent and helpful in kidney trauma.

No MeSH data available.


Related in: MedlinePlus