Limits...
Protective effects of Carissa opaca fruits against CCl4-induced oxidative kidney lipid peroxidation and trauma in rat.

Sahreen S, Khan MR, Khan RA, Alkreathy HM - Food Nutr Res (2015)

Bottom Line: The protective effects of various fractions of C. opaca fruits against CCl4 administration were reviewed by rat renal function alterations.Chronic toxicity caused by 8-week treatment of CCl4 to the rats significantly decreased the pH level, activities of antioxidant enzymes, and glutathione contents, whereas a significant increase was found in the case of specific gravity, red blood cells, white blood cells, level of urea, and lipid peroxidation in comparison to control group.Results revealed that the methanolic fractions of C. opaca are the most potent and helpful in kidney trauma.

View Article: PubMed Central - PubMed

Affiliation: Botanical Sciences Division, Pakistan Museum of Natural History, Islamabad, Pakistan.

ABSTRACT

Background: Carbon tetrachloride (CCl4) is a potent nephrotoxin, as it causes acute as well as chronic toxicity in kidneys. Therefore, this study was carried out to assess the pharmacological potential of different fractions of Carissa opaca fruits on CCl4-induced oxidative trauma in the kidney.

Methods: The parameters studied in this respect were the kidney function tests viz, serum profile, urine profile, genotoxicity, characteristic morphological findings, and antioxidant enzymatic level of kidneys.

Result: The protective effects of various fractions of C. opaca fruits against CCl4 administration were reviewed by rat renal function alterations. Chronic toxicity caused by 8-week treatment of CCl4 to the rats significantly decreased the pH level, activities of antioxidant enzymes, and glutathione contents, whereas a significant increase was found in the case of specific gravity, red blood cells, white blood cells, level of urea, and lipid peroxidation in comparison to control group. Administration of various fractions of C. opaca fruit with CCl4 showed protective ability against CCl4 intoxication by restoring the urine profile, activities of antioxidant enzymes, and lipid peroxidation in rat. CCl4 induction in rats also caused DNA fragmentation and glomerular atrophy by means of dilation, disappearance of Bowmen's space, congestion in the capillary loops, dilation in renal tubules, and foamy look of epithelial cells of tubular region, which were restored by co-admiration of various fractions of C. opaca.

Conclusion: Results revealed that the methanolic fractions of C. opaca are the most potent and helpful in kidney trauma.

No MeSH data available.


Related in: MedlinePlus

Agarose gel showing DNA damage by CCl4 and protective effects of various fractions of C. opaca fruits in renal tissue. Lanes from left: (M) low molecular weight marker, (1) control, (2) DMSO+Olive oil group, (3) CCl4 group, (4) silymarin+CCl4 group, (5) MFC+CCl4 group, (6) EFC+CCl4 group, (7) HFC+CCl4 group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4563101&req=5

Figure 0001: Agarose gel showing DNA damage by CCl4 and protective effects of various fractions of C. opaca fruits in renal tissue. Lanes from left: (M) low molecular weight marker, (1) control, (2) DMSO+Olive oil group, (3) CCl4 group, (4) silymarin+CCl4 group, (5) MFC+CCl4 group, (6) EFC+CCl4 group, (7) HFC+CCl4 group.

Mentions: DNA was extracted from renal tissues of the treated rats by a stepwise method and then was loaded on 1.5% agarose gel. Figure 1 details the banding pattern of DNA. In case of CCl4-intoxicated rats, a peculiar fragmentation of DNA can be observed, which was absent from the renal tissues of control rats. DNA extracted from the group treated with various fractions of C. opaca fruits along with CCl4 showed a markedly repaired DNA. The groups treated with only various fractions did not show any kind of DNA damage.


Protective effects of Carissa opaca fruits against CCl4-induced oxidative kidney lipid peroxidation and trauma in rat.

Sahreen S, Khan MR, Khan RA, Alkreathy HM - Food Nutr Res (2015)

Agarose gel showing DNA damage by CCl4 and protective effects of various fractions of C. opaca fruits in renal tissue. Lanes from left: (M) low molecular weight marker, (1) control, (2) DMSO+Olive oil group, (3) CCl4 group, (4) silymarin+CCl4 group, (5) MFC+CCl4 group, (6) EFC+CCl4 group, (7) HFC+CCl4 group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4563101&req=5

Figure 0001: Agarose gel showing DNA damage by CCl4 and protective effects of various fractions of C. opaca fruits in renal tissue. Lanes from left: (M) low molecular weight marker, (1) control, (2) DMSO+Olive oil group, (3) CCl4 group, (4) silymarin+CCl4 group, (5) MFC+CCl4 group, (6) EFC+CCl4 group, (7) HFC+CCl4 group.
Mentions: DNA was extracted from renal tissues of the treated rats by a stepwise method and then was loaded on 1.5% agarose gel. Figure 1 details the banding pattern of DNA. In case of CCl4-intoxicated rats, a peculiar fragmentation of DNA can be observed, which was absent from the renal tissues of control rats. DNA extracted from the group treated with various fractions of C. opaca fruits along with CCl4 showed a markedly repaired DNA. The groups treated with only various fractions did not show any kind of DNA damage.

Bottom Line: The protective effects of various fractions of C. opaca fruits against CCl4 administration were reviewed by rat renal function alterations.Chronic toxicity caused by 8-week treatment of CCl4 to the rats significantly decreased the pH level, activities of antioxidant enzymes, and glutathione contents, whereas a significant increase was found in the case of specific gravity, red blood cells, white blood cells, level of urea, and lipid peroxidation in comparison to control group.Results revealed that the methanolic fractions of C. opaca are the most potent and helpful in kidney trauma.

View Article: PubMed Central - PubMed

Affiliation: Botanical Sciences Division, Pakistan Museum of Natural History, Islamabad, Pakistan.

ABSTRACT

Background: Carbon tetrachloride (CCl4) is a potent nephrotoxin, as it causes acute as well as chronic toxicity in kidneys. Therefore, this study was carried out to assess the pharmacological potential of different fractions of Carissa opaca fruits on CCl4-induced oxidative trauma in the kidney.

Methods: The parameters studied in this respect were the kidney function tests viz, serum profile, urine profile, genotoxicity, characteristic morphological findings, and antioxidant enzymatic level of kidneys.

Result: The protective effects of various fractions of C. opaca fruits against CCl4 administration were reviewed by rat renal function alterations. Chronic toxicity caused by 8-week treatment of CCl4 to the rats significantly decreased the pH level, activities of antioxidant enzymes, and glutathione contents, whereas a significant increase was found in the case of specific gravity, red blood cells, white blood cells, level of urea, and lipid peroxidation in comparison to control group. Administration of various fractions of C. opaca fruit with CCl4 showed protective ability against CCl4 intoxication by restoring the urine profile, activities of antioxidant enzymes, and lipid peroxidation in rat. CCl4 induction in rats also caused DNA fragmentation and glomerular atrophy by means of dilation, disappearance of Bowmen's space, congestion in the capillary loops, dilation in renal tubules, and foamy look of epithelial cells of tubular region, which were restored by co-admiration of various fractions of C. opaca.

Conclusion: Results revealed that the methanolic fractions of C. opaca are the most potent and helpful in kidney trauma.

No MeSH data available.


Related in: MedlinePlus