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Stimulation of Innate and Adaptive Immunity by Using Filamentous Bacteriophage fd Targeted to DEC-205.

D'Apice L, Costa V, Sartorius R, Trovato M, Aprile M, De Berardinis P - J Immunol Res (2015)

Bottom Line: The filamentous bacteriophage fd, codisplaying antigenic determinants and a single chain antibody fragment directed against the dendritic cell receptor DEC-205, is a promising vaccine candidate for its safety and its ability to elicit innate and adaptive immune response in absence of adjuvants.By using a system vaccinology approach based on RNA-Sequencing (RNA-Seq) analysis, we describe a relevant gene modulation in dendritic cells pulsed with anti-DEC-205 bacteriophages fd.RNA-Seq data analysis indicates that the bacteriophage fd virions are sensed as a pathogen by dendritic cells; they activate the danger receptors that trigger an innate immune response and thus confer a strong adjuvanticity that is needed to obtain a long-lasting adaptive immune response.

View Article: PubMed Central - PubMed

Affiliation: Institute of Protein Biochemistry (IBP), National Council of Research, 80131 Naples, Italy.

ABSTRACT
The filamentous bacteriophage fd, codisplaying antigenic determinants and a single chain antibody fragment directed against the dendritic cell receptor DEC-205, is a promising vaccine candidate for its safety and its ability to elicit innate and adaptive immune response in absence of adjuvants. By using a system vaccinology approach based on RNA-Sequencing (RNA-Seq) analysis, we describe a relevant gene modulation in dendritic cells pulsed with anti-DEC-205 bacteriophages fd. RNA-Seq data analysis indicates that the bacteriophage fd virions are sensed as a pathogen by dendritic cells; they activate the danger receptors that trigger an innate immune response and thus confer a strong adjuvanticity that is needed to obtain a long-lasting adaptive immune response.

No MeSH data available.


Related in: MedlinePlus

OVA specific OT-I CD8 T cell proliferation. (a) Flow cytometry strategy analysis of OT-I T cells proliferation. One representative sample per group is reported. P1: live cells, P2: OT-I CD8+ Vα2+ T cells, and P3: OT-I CD8+ Vα2+ proliferating cells with CFSE fluorescence intensity <104 and >102. The CFSE fluorescence intensity of the P3 population is reported in the histograms and numbers represent the percentage of proliferating cells. Peaks represent the cell division. (b) Mean ± SD percentage of proliferating T cells from adoptively transferred mice (n = 5) immunised with PBS or fdOVA/sc-αDEC bacteriophage is shown. ∗p < 0.01.
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fig2: OVA specific OT-I CD8 T cell proliferation. (a) Flow cytometry strategy analysis of OT-I T cells proliferation. One representative sample per group is reported. P1: live cells, P2: OT-I CD8+ Vα2+ T cells, and P3: OT-I CD8+ Vα2+ proliferating cells with CFSE fluorescence intensity <104 and >102. The CFSE fluorescence intensity of the P3 population is reported in the histograms and numbers represent the percentage of proliferating cells. Peaks represent the cell division. (b) Mean ± SD percentage of proliferating T cells from adoptively transferred mice (n = 5) immunised with PBS or fdOVA/sc-αDEC bacteriophage is shown. ∗p < 0.01.

Mentions: As reported in Figure 2 the fdOVA/sc-αDEC induced a strong proliferative response of the OVA-specific CD8+ T cells, in absence of exogenous adjuvant.


Stimulation of Innate and Adaptive Immunity by Using Filamentous Bacteriophage fd Targeted to DEC-205.

D'Apice L, Costa V, Sartorius R, Trovato M, Aprile M, De Berardinis P - J Immunol Res (2015)

OVA specific OT-I CD8 T cell proliferation. (a) Flow cytometry strategy analysis of OT-I T cells proliferation. One representative sample per group is reported. P1: live cells, P2: OT-I CD8+ Vα2+ T cells, and P3: OT-I CD8+ Vα2+ proliferating cells with CFSE fluorescence intensity <104 and >102. The CFSE fluorescence intensity of the P3 population is reported in the histograms and numbers represent the percentage of proliferating cells. Peaks represent the cell division. (b) Mean ± SD percentage of proliferating T cells from adoptively transferred mice (n = 5) immunised with PBS or fdOVA/sc-αDEC bacteriophage is shown. ∗p < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4563097&req=5

fig2: OVA specific OT-I CD8 T cell proliferation. (a) Flow cytometry strategy analysis of OT-I T cells proliferation. One representative sample per group is reported. P1: live cells, P2: OT-I CD8+ Vα2+ T cells, and P3: OT-I CD8+ Vα2+ proliferating cells with CFSE fluorescence intensity <104 and >102. The CFSE fluorescence intensity of the P3 population is reported in the histograms and numbers represent the percentage of proliferating cells. Peaks represent the cell division. (b) Mean ± SD percentage of proliferating T cells from adoptively transferred mice (n = 5) immunised with PBS or fdOVA/sc-αDEC bacteriophage is shown. ∗p < 0.01.
Mentions: As reported in Figure 2 the fdOVA/sc-αDEC induced a strong proliferative response of the OVA-specific CD8+ T cells, in absence of exogenous adjuvant.

Bottom Line: The filamentous bacteriophage fd, codisplaying antigenic determinants and a single chain antibody fragment directed against the dendritic cell receptor DEC-205, is a promising vaccine candidate for its safety and its ability to elicit innate and adaptive immune response in absence of adjuvants.By using a system vaccinology approach based on RNA-Sequencing (RNA-Seq) analysis, we describe a relevant gene modulation in dendritic cells pulsed with anti-DEC-205 bacteriophages fd.RNA-Seq data analysis indicates that the bacteriophage fd virions are sensed as a pathogen by dendritic cells; they activate the danger receptors that trigger an innate immune response and thus confer a strong adjuvanticity that is needed to obtain a long-lasting adaptive immune response.

View Article: PubMed Central - PubMed

Affiliation: Institute of Protein Biochemistry (IBP), National Council of Research, 80131 Naples, Italy.

ABSTRACT
The filamentous bacteriophage fd, codisplaying antigenic determinants and a single chain antibody fragment directed against the dendritic cell receptor DEC-205, is a promising vaccine candidate for its safety and its ability to elicit innate and adaptive immune response in absence of adjuvants. By using a system vaccinology approach based on RNA-Sequencing (RNA-Seq) analysis, we describe a relevant gene modulation in dendritic cells pulsed with anti-DEC-205 bacteriophages fd. RNA-Seq data analysis indicates that the bacteriophage fd virions are sensed as a pathogen by dendritic cells; they activate the danger receptors that trigger an innate immune response and thus confer a strong adjuvanticity that is needed to obtain a long-lasting adaptive immune response.

No MeSH data available.


Related in: MedlinePlus