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Investigation of Stilbenoids as Potential Therapeutic Agents for Rotavirus Gastroenteritis.

Ball JM, Medina-Bolivar F, Defrates K, Hambleton E, Hurlburt ME, Fang L, Yang T, Nopo-Olazabal L, Atwill RL, Ghai P, Parr RD - Adv Virol (2015)

Bottom Line: Cell viability counts showed no cytotoxic effects on HT29.f8 cells.Two stilbenoids, trans-arachidin-1 and trans-arachidin-3, show a significant decrease in RV infectivity titers.Western blot analyses performed on the infected cell lysates complemented the infectivity titrations and indicated a significant decrease in viral replication.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathobiology, Texas A&M University, College Station, TX 77843, USA.

ABSTRACT
Rotavirus (RV) infections cause severe diarrhea in infants and young children worldwide. Vaccines are available but cost prohibitive for many countries and only reduce severe symptoms. Vaccinated infants continue to shed infectious particles, and studies show decreased efficacy of the RV vaccines in tropical and subtropical countries where they are needed most. Continuing surveillance for new RV strains, assessment of vaccine efficacy, and development of cost effective antiviral drugs remain an important aspect of RV studies. This study was to determine the efficacy of antioxidant and anti-inflammatory stilbenoids to inhibit RV replication. Peanut (A. hypogaea) hairy root cultures were induced to produce stilbenoids, which were purified by high performance countercurrent chromatography (HPCCC) and analyzed by HPLC. HT29.f8 cells were infected with RV in the presence stilbenoids. Cell viability counts showed no cytotoxic effects on HT29.f8 cells. Viral infectivity titers were calculated and comparatively assessed to determine the effects of stilbenoid treatments. Two stilbenoids, trans-arachidin-1 and trans-arachidin-3, show a significant decrease in RV infectivity titers. Western blot analyses performed on the infected cell lysates complemented the infectivity titrations and indicated a significant decrease in viral replication. These studies show the therapeutic potential of the stilbenoids against RV replication.

No MeSH data available.


Related in: MedlinePlus

Chemical structures of the four stilbenoids tested. All compounds are shown in trans-isomers. (a) Resveratrol (t-Res). (b) Piceatannol (t-Pa). (c) Arachidin-3 (t-A3). (d) Arachidin-1 (t-A1).
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fig1: Chemical structures of the four stilbenoids tested. All compounds are shown in trans-isomers. (a) Resveratrol (t-Res). (b) Piceatannol (t-Pa). (c) Arachidin-3 (t-A3). (d) Arachidin-1 (t-A1).

Mentions: Stilbenoids are produced by a group of plants which includes grapes, peanuts, and some berries [12, 16, 17]. trans-Piceatannol (t-PA) is a hydroxylated analog of resveratrol found in grapes and in minor quantities in peanuts. trans-Arachidin-1 (t-A1) is a prenylated (3-methyl-1-butenyl) analog of piceatannol, whereas trans-arachidin-3 (t-A3) is a prenylated (3-methyl-1-butenyl) analog of resveratrol (Figures 1(a)–1(d)). Both t-A1 and t-A3 are produced in peanuts upon fungal challenge. These stilbenoids can be extracted from some plants but are not suitable for many applications in the food/pharmaceutical sectors due to the overall low concentration of stilbenoids in the plant extracts. To deliver a highly defined and stilbenoid-enriched product, hairy root cultures of peanut (A. hypogaea) have been established in a bioproduction system that produces increased levels of stilbenoids, including t-A1 and t-A3, upon treatment with elicitors [18, 19]. t-PA and t-Res are commercially available, but t-A1 and t-A3 are still in an experimental stage resulting in an opportunity to explore new antiviral biological activity.


Investigation of Stilbenoids as Potential Therapeutic Agents for Rotavirus Gastroenteritis.

Ball JM, Medina-Bolivar F, Defrates K, Hambleton E, Hurlburt ME, Fang L, Yang T, Nopo-Olazabal L, Atwill RL, Ghai P, Parr RD - Adv Virol (2015)

Chemical structures of the four stilbenoids tested. All compounds are shown in trans-isomers. (a) Resveratrol (t-Res). (b) Piceatannol (t-Pa). (c) Arachidin-3 (t-A3). (d) Arachidin-1 (t-A1).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4563088&req=5

fig1: Chemical structures of the four stilbenoids tested. All compounds are shown in trans-isomers. (a) Resveratrol (t-Res). (b) Piceatannol (t-Pa). (c) Arachidin-3 (t-A3). (d) Arachidin-1 (t-A1).
Mentions: Stilbenoids are produced by a group of plants which includes grapes, peanuts, and some berries [12, 16, 17]. trans-Piceatannol (t-PA) is a hydroxylated analog of resveratrol found in grapes and in minor quantities in peanuts. trans-Arachidin-1 (t-A1) is a prenylated (3-methyl-1-butenyl) analog of piceatannol, whereas trans-arachidin-3 (t-A3) is a prenylated (3-methyl-1-butenyl) analog of resveratrol (Figures 1(a)–1(d)). Both t-A1 and t-A3 are produced in peanuts upon fungal challenge. These stilbenoids can be extracted from some plants but are not suitable for many applications in the food/pharmaceutical sectors due to the overall low concentration of stilbenoids in the plant extracts. To deliver a highly defined and stilbenoid-enriched product, hairy root cultures of peanut (A. hypogaea) have been established in a bioproduction system that produces increased levels of stilbenoids, including t-A1 and t-A3, upon treatment with elicitors [18, 19]. t-PA and t-Res are commercially available, but t-A1 and t-A3 are still in an experimental stage resulting in an opportunity to explore new antiviral biological activity.

Bottom Line: Cell viability counts showed no cytotoxic effects on HT29.f8 cells.Two stilbenoids, trans-arachidin-1 and trans-arachidin-3, show a significant decrease in RV infectivity titers.Western blot analyses performed on the infected cell lysates complemented the infectivity titrations and indicated a significant decrease in viral replication.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathobiology, Texas A&M University, College Station, TX 77843, USA.

ABSTRACT
Rotavirus (RV) infections cause severe diarrhea in infants and young children worldwide. Vaccines are available but cost prohibitive for many countries and only reduce severe symptoms. Vaccinated infants continue to shed infectious particles, and studies show decreased efficacy of the RV vaccines in tropical and subtropical countries where they are needed most. Continuing surveillance for new RV strains, assessment of vaccine efficacy, and development of cost effective antiviral drugs remain an important aspect of RV studies. This study was to determine the efficacy of antioxidant and anti-inflammatory stilbenoids to inhibit RV replication. Peanut (A. hypogaea) hairy root cultures were induced to produce stilbenoids, which were purified by high performance countercurrent chromatography (HPCCC) and analyzed by HPLC. HT29.f8 cells were infected with RV in the presence stilbenoids. Cell viability counts showed no cytotoxic effects on HT29.f8 cells. Viral infectivity titers were calculated and comparatively assessed to determine the effects of stilbenoid treatments. Two stilbenoids, trans-arachidin-1 and trans-arachidin-3, show a significant decrease in RV infectivity titers. Western blot analyses performed on the infected cell lysates complemented the infectivity titrations and indicated a significant decrease in viral replication. These studies show the therapeutic potential of the stilbenoids against RV replication.

No MeSH data available.


Related in: MedlinePlus