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Temporary inactivation of the anterior part of the bed nucleus of the stria terminalis blocks alarm pheromone-induced defensive behavior in rats.

Breitfeld T, Bruning JE, Inagaki H, Takeuchi Y, Kiyokawa Y, Fendt M - Front Neurosci (2015)

Bottom Line: One of these brain areas is the anterior bed nucleus of the stria terminalis (aBNST).The goal of the present study was to investigate if pharmacological inactivation of the aBNST by local microinjections of the GABAA receptor-agonist muscimol modulates alarm pheromone-induced defensive behaviors.Our data show that temporary inactivation of the aBNST blocked head out behavior in response to the alarm pheromone.

View Article: PubMed Central - PubMed

Affiliation: Institute for Pharmacology and Toxicology, Otto-von-Guericke University Magdeburg Magdeburg, Germany.

ABSTRACT
Rats emit an alarm pheromone in threatening situations. Exposure of rats to this alarm pheromone induces defensive behaviors, such as head out behavior, and increases c-Fos expression in brain areas involved in the mediation of defensive behaviors. One of these brain areas is the anterior bed nucleus of the stria terminalis (aBNST). The goal of the present study was to investigate if pharmacological inactivation of the aBNST by local microinjections of the GABAA receptor-agonist muscimol modulates alarm pheromone-induced defensive behaviors. We first established the behavioral paradigm of alarm pheromone-induced defensive behaviors in Sprague-Dawley rats in our laboratory. In a second experiment, we inactivated the aBNST, then exposed rats to one of four different odors (neck odor, female urine, alarm pheromone, fox urine) and tested the effects of the aBNST inactivation on the behavior in response to these odors. Our data show that temporary inactivation of the aBNST blocked head out behavior in response to the alarm pheromone. This indicates that the aBNST plays an important role in the mediation of the alarm pheromone-induced defensive behavior in rats.

No MeSH data available.


Related in: MedlinePlus

Injection sites into the aBNST. (A) Reconstruction of the different injection sites of saline or muscimol into the aBNST. The coronal sections were taken from the atlas of Paxinos and Watson (2014). Numbers indicate distance from bregma in mm. aBNST, anterior bed nucleus of the stria terminalis; MnPo, median preoptic nucleus; MS, medial septal nucleus; CPu, caudate putamen; LPO, lateral preoptic area; VP, ventral pallidum; f, fornix. (B) Photomicrographs with a representative example of aBNST injection sites.
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Figure 2: Injection sites into the aBNST. (A) Reconstruction of the different injection sites of saline or muscimol into the aBNST. The coronal sections were taken from the atlas of Paxinos and Watson (2014). Numbers indicate distance from bregma in mm. aBNST, anterior bed nucleus of the stria terminalis; MnPo, median preoptic nucleus; MS, medial septal nucleus; CPu, caudate putamen; LPO, lateral preoptic area; VP, ventral pallidum; f, fornix. (B) Photomicrographs with a representative example of aBNST injection sites.

Mentions: Histological analysis of the injections sites revealed that 19 rats received bilateral injections into the aBNST (saline: n = 10; muscimol: n = 9), consisting of the anterior, dorsal and lateral divisions of the BNST (see Figure 2). Some animals had to be excluded from the analysis because of misplaced injections (n = 12) (lateral ventricle, medial preoptic area, caudate putamen, nucleus accumbens, lateral preoptic area, parastrial nucleus, intermediate lateral septal nucleus, medial preoptic nucleus, ventrolateral preoptic nucleus), lesions in the injection area (n = 5), or abnormal behavior after muscimol injections (rotation behavior; n = 6).


Temporary inactivation of the anterior part of the bed nucleus of the stria terminalis blocks alarm pheromone-induced defensive behavior in rats.

Breitfeld T, Bruning JE, Inagaki H, Takeuchi Y, Kiyokawa Y, Fendt M - Front Neurosci (2015)

Injection sites into the aBNST. (A) Reconstruction of the different injection sites of saline or muscimol into the aBNST. The coronal sections were taken from the atlas of Paxinos and Watson (2014). Numbers indicate distance from bregma in mm. aBNST, anterior bed nucleus of the stria terminalis; MnPo, median preoptic nucleus; MS, medial septal nucleus; CPu, caudate putamen; LPO, lateral preoptic area; VP, ventral pallidum; f, fornix. (B) Photomicrographs with a representative example of aBNST injection sites.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4563084&req=5

Figure 2: Injection sites into the aBNST. (A) Reconstruction of the different injection sites of saline or muscimol into the aBNST. The coronal sections were taken from the atlas of Paxinos and Watson (2014). Numbers indicate distance from bregma in mm. aBNST, anterior bed nucleus of the stria terminalis; MnPo, median preoptic nucleus; MS, medial septal nucleus; CPu, caudate putamen; LPO, lateral preoptic area; VP, ventral pallidum; f, fornix. (B) Photomicrographs with a representative example of aBNST injection sites.
Mentions: Histological analysis of the injections sites revealed that 19 rats received bilateral injections into the aBNST (saline: n = 10; muscimol: n = 9), consisting of the anterior, dorsal and lateral divisions of the BNST (see Figure 2). Some animals had to be excluded from the analysis because of misplaced injections (n = 12) (lateral ventricle, medial preoptic area, caudate putamen, nucleus accumbens, lateral preoptic area, parastrial nucleus, intermediate lateral septal nucleus, medial preoptic nucleus, ventrolateral preoptic nucleus), lesions in the injection area (n = 5), or abnormal behavior after muscimol injections (rotation behavior; n = 6).

Bottom Line: One of these brain areas is the anterior bed nucleus of the stria terminalis (aBNST).The goal of the present study was to investigate if pharmacological inactivation of the aBNST by local microinjections of the GABAA receptor-agonist muscimol modulates alarm pheromone-induced defensive behaviors.Our data show that temporary inactivation of the aBNST blocked head out behavior in response to the alarm pheromone.

View Article: PubMed Central - PubMed

Affiliation: Institute for Pharmacology and Toxicology, Otto-von-Guericke University Magdeburg Magdeburg, Germany.

ABSTRACT
Rats emit an alarm pheromone in threatening situations. Exposure of rats to this alarm pheromone induces defensive behaviors, such as head out behavior, and increases c-Fos expression in brain areas involved in the mediation of defensive behaviors. One of these brain areas is the anterior bed nucleus of the stria terminalis (aBNST). The goal of the present study was to investigate if pharmacological inactivation of the aBNST by local microinjections of the GABAA receptor-agonist muscimol modulates alarm pheromone-induced defensive behaviors. We first established the behavioral paradigm of alarm pheromone-induced defensive behaviors in Sprague-Dawley rats in our laboratory. In a second experiment, we inactivated the aBNST, then exposed rats to one of four different odors (neck odor, female urine, alarm pheromone, fox urine) and tested the effects of the aBNST inactivation on the behavior in response to these odors. Our data show that temporary inactivation of the aBNST blocked head out behavior in response to the alarm pheromone. This indicates that the aBNST plays an important role in the mediation of the alarm pheromone-induced defensive behavior in rats.

No MeSH data available.


Related in: MedlinePlus